Structural dynamics of the intrinsically disordered linker region of cardiac troponin T.

Autor: Cubuk J; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA.; Center for Biomolecular Condensates, Washington University in St Louis, 1 Brookings Drive, 63130, Saint Louis, MO, USA., Greenberg L; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA., Greenberg AE; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA., Emenecker RJ; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA.; Center for Biomolecular Condensates, Washington University in St Louis, 1 Brookings Drive, 63130, Saint Louis, MO, USA., Stuchell-Brereton MD; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA.; Center for Biomolecular Condensates, Washington University in St Louis, 1 Brookings Drive, 63130, Saint Louis, MO, USA., Holehouse AS; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA.; Center for Biomolecular Condensates, Washington University in St Louis, 1 Brookings Drive, 63130, Saint Louis, MO, USA., Soranno A; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA.; Center for Biomolecular Condensates, Washington University in St Louis, 1 Brookings Drive, 63130, Saint Louis, MO, USA., Greenberg MJ; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 Euclid Ave, 63110, Saint Louis, MO, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Oct 14. Date of Electronic Publication: 2024 Oct 14.

Accounting for fast vs slow exchange in single molecule FRET experiments reveals hidden conformational states.

Autor: Miller JJ; Departments of Biochemistry & Biophysics and Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, United States.; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States., Mallimadugula UL; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States., Zimmerman MI; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States., Stuchell-Brereton MD; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States., Soranno A; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States., Bowman GR; Departments of Biochemistry & Biophysics and Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, United States.; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 03. Date of Electronic Publication: 2024 Jun 03.

Biophysical Mechanism of Allosteric Regulation of Actin Capping Protein.

Autor: Mooren OL; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, United States., Stuchell-Brereton MD; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, United States; Center for Biomolecular Condensates, Washington University in St Louis, St. Louis, MO, United States., McConnell P; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, United States., Yan C; Department of Biophysics, University of Michigan, Ann Arbor, MI, United States., Wilkerson EM; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, United States; Institute for Informatics, Washington University School of Medicine, St. Louis, MO, United States., Goldfarb D; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, United States; Institute for Informatics, Washington University School of Medicine, St. Louis, MO, United States., Cooper JA; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, United States. Electronic address: jacooper@wustl.edu., Sept D; Department of Biophysics, University of Michigan, Ann Arbor, MI, United States; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, United States. Electronic address: dsept@umich.edu., Soranno A; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, United States; Center for Biomolecular Condensates, Washington University in St Louis, St. Louis, MO, United States. Electronic address: soranno@wustl.edu.

Publikováno v: Journal of molecular biology [J Mol Biol] 2023 Dec 15; Vol. 435 (24), pp. 168342. Date of Electronic Publication: 2023 Nov 02.

Apolipoprotein E4 has extensive conformational heterogeneity in lipid-free and lipid-bound forms.

Autor: Stuchell-Brereton MD; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Zimmerman MI; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Miller JJ; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Mallimadugula UL; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Incicco JJ; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Roy D; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Smith LG; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Cubuk J; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Baban B; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110., DeKoster GT; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110., Frieden C; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110., Bowman GR; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130., Soranno A; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, Saint Louis, MO 63110.; Center for Science and Engineering of Living Systems, Washington University in St. Louis, Saint Louis, MO 63130.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Feb 14; Vol. 120 (7), pp. e2215371120. Date of Electronic Publication: 2023 Feb 07.

The biophysics of disordered proteins from the point of view of single-molecule fluorescence spectroscopy.

Autor: Cubuk J; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 St Euclid Ave, Saint Louis, MO 63110, U.S.A.; Center for Science and Engineering of Living Systems, Washington University in St Louis, 1 Brookings Drive, Saint Louis, MO 63130, U.S.A., Stuchell-Brereton MD; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 St Euclid Ave, Saint Louis, MO 63110, U.S.A.; Center for Science and Engineering of Living Systems, Washington University in St Louis, 1 Brookings Drive, Saint Louis, MO 63130, U.S.A., Soranno A; Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, 660 St Euclid Ave, Saint Louis, MO 63110, U.S.A.; Center for Science and Engineering of Living Systems, Washington University in St Louis, 1 Brookings Drive, Saint Louis, MO 63130, U.S.A.

Publikováno v: Essays in biochemistry [Essays Biochem] 2022 Dec 16; Vol. 66 (7), pp. 875-890.