Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Stuart E. Builder"'
Principles of process validation are extremely powerful tools in assurance of product quality. They are especially useful for reducing those risks not easily measured routinely during production. When combined with effective process and facility desi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9cb43874c12a92329e83505571f0f2a9
https://doi.org/10.1201/9780203749166-19
https://doi.org/10.1201/9780203749166-19
Publikováno v:
Biotechnology Set, Second Edition
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8403b3d5b8698790a4d58a37200278a
https://doi.org/10.1002/9783527620999.ch4b
https://doi.org/10.1002/9783527620999.ch4b
Publikováno v:
Journal of Chromatography A. 753:73-80
Adsorption chromatography using underivatized porous glass can be an effective capture step for the purification of recombinant proteins. Classical desorption techniques using chaotropic agents or harsh chemical solvents often result in elution of in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28439ad9b377cbe025f54ae8c64d9ee7
https://doi.org/10.1016/s0021-9673(96)00549-3
https://doi.org/10.1016/s0021-9673(96)00549-3
Autor:
Wassim. Nashabeh, Dan. Kirby, David Reifsnyder, Barry L. Karger, František Foret, Kimberly F. Greve, Stuart E. Builder
Publikováno v:
Analytical Chemistry. 66:2148-2154
A highly selective electrophoretic system employing differential hydrophobic interaction was evaluated for the quantitative determination of recombinant insulin-like growth factor I (IGF-I) variants. The system consisted of mixed aqueous-organic buff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a21c2e179272bb3b9fc2a2407e93ab01
https://doi.org/10.1021/ac00085a033
https://doi.org/10.1021/ac00085a033
Publikováno v:
Journal of Chromatography A. 675:101-112
The isolation of recombinant human insulin-like growth factor 1 (rhIGF-1) is complicated by the presence of several rhIGF-1 variants which co-purify using conventional chromatographic media. These species consist primarily of a methionine-sulfoxide v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1a49d44ded3bdb4ffdb6d2c0ae5eb34
https://doi.org/10.1016/0021-9673(94)85264-2
https://doi.org/10.1016/0021-9673(94)85264-2
Publikováno v:
Nature Biotechnology. 10:182-188
We describe a new class of membrane that has the capability of removing particles such as viruses from solution with resolution and reproducibility superior to that of conventional membranes. This composite membrane is composed of a pre-formed microp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfaa2b30fedfeaf34b187efca86cb046
https://doi.org/10.1038/nbt0292-182
https://doi.org/10.1038/nbt0292-182
Publikováno v:
Biotechnology and Bioengineering. 38:413-422
An industrial-scale methods for harvest of biologically active proteins form mammalian cell culture has been developed using tangential flow filtration. A robust and economical process capable of processing approximately 5000 L conditioned media/h wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7bceaa98454e5bdeb224b0737761bc10
https://doi.org/10.1002/bit.260380411
https://doi.org/10.1002/bit.260380411
Publikováno v:
Bio/technology (Nature Publishing Company). 12(11)
Human insulin-like growth factor I (IGF-I) accumulates in both folded and aggregated forms in the fermentation medium and cellular periplasmic space when expressed in E. coli with an endogenous secretory signal sequence. Due to its heterogeneity in f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad00e011e8ac11f38cbe58be35add12a
https://pubmed.ncbi.nlm.nih.gov/7765554
https://pubmed.ncbi.nlm.nih.gov/7765554
Autor:
Jeffrey L. Cleland, Stuart E. Builder, Daniel I. C. Wang, Judy Yi-Huei Chang, Marjorie E. Winkler, James R. Swartz
Publikováno v:
Bio/technology (Nature Publishing Company). 10(9)
Previous studies on the refolding of recombinant bovine carbonic anhydrase B (CAB) indicated that polyethylene glycol (PEG) significantly enhanced the recovery of active protein by reducing aggregation. To further test the ability of PEG to enhance r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c45735d9df7505b25eea249d9a7548fa
https://pubmed.ncbi.nlm.nih.gov/1368998
https://pubmed.ncbi.nlm.nih.gov/1368998