Remote Homology Detection Identifies a Eukaryotic RPA DBD-C-like DNA Binding Domain as a Conserved Feature of Archaeal Rpa1-Like Proteins

Autor: Stuart A. MacNeill

Publikováno v: Frontiers in Molecular Biosciences, Vol 8 (2021)

The eukaryotic single-stranded DNA binding factor replication protein A (RPA) is essential for DNA replication, repair and recombination. RPA is a heterotrimer containing six related OB folds and a winged helix-turn-helix (wH) domain. The OB folds ar

Externí odkaz: https://doaj.org/article/3fe8f120e489426fbcce883cf3cee1ca

Identification of a Candidate Rad1 Subunit for the Kinetoplastid 9-1-1 (Rad9-Hus1-Rad1) Complex

Autor: Stuart A. MacNeill

Publikováno v: Biology, Vol 3, Iss 4, Pp 922-927 (2014)

The trimeric 9-1-1 (Rad9-Hus1-Rad1) complex plays an important role in the eukaryotic DNA damage response by recruiting DNA repair factors and checkpoint mediators to damaged sites. Extensively characterised in mammals and yeast, evidence is now emer

Externí odkaz: https://doaj.org/article/bc96f0a64e3f4914a78caac61074ce89

Non-canonical binding of the Chaetomium thermophilum PolD4 N-terminal PIP motif to PCNA involves Q-pocket and compact 2-fork plug interactions but no 3

Autor: Dongxiao, Yang, Magnus S, Alphey, Stuart A, MacNeill

Publikováno v: The FEBS journalReferences.

DNA polymerase δ (Pol δ) is a key enzyme for the maintenance of genome integrity in eukaryotic cells, acting in concert with the sliding clamp processivity factor PCNA (proliferating cell nuclear antigen). Three of the four subunits of human Pol δ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2f02b183e16e51e13f7328314cba33e2
https://pubmed.ncbi.nlm.nih.gov/35942639

Single-Stranded DNA-Binding Proteins in the Archaea

Autor: Simonetta Gribaldo, Najwa Taib, Stuart A. MacNeill

Publikováno v: Single Stranded DNA Binding Proteins
Single Stranded DNA Binding Proteins, 2281, Springer US; Springer US, pp.23-47, 2021, Methods in Molecular Biology, 978-1-07-161290-3. ⟨10.1007/978-1-0716-1290-3_2⟩
Methods in Molecular Biology ISBN: 9781071612897

International audience; Single-stranded (ss) DNA-binding proteins are found in all three domains of life where they play vital roles in nearly all aspects of DNA metabolism by binding to and stabilizing exposed ssDNA and acting as platforms onto whic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64cfddb15a8fe9bcf38ea2bb8db73dfc
https://hal-pasteur.archives-ouvertes.fr/pasteur-03682183/document

The archaeal RecJ-like proteins: nucleases and ex-nucleases with diverse roles in replication and repair

Autor: Stuart A. MacNeill

Publikováno v: Emerging Topics in Life Sciences. 2:493-501

RecJ proteins belong to the DHH superfamily of phosphoesterases that has members in all three domains of life. In bacteria, the archetypal RecJ is a 5′ → 3′ ssDNA exonuclease that functions in homologous recombination, base excision repair and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f772c861506e047d01d777f8c847f55d
https://doi.org/10.1042/etls20180017