Engineering an inducible leukemia-associated fusion protein enables large-scale ex vivo production of functional human phagocytes.

Autor: Windisch R; Division of Transfusion Medicine, Cell Therapeutics and Haemostaseology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Soliman S; Division of Transfusion Medicine, Cell Therapeutics and Haemostaseology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Hoffmann A; Vascular Biology, Institute for Stroke and Dementia Research, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; Department of Anesthesiology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Chen-Wichmann L; Division of Transfusion Medicine, Cell Therapeutics and Haemostaseology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Danese A; Biomedical Center, Department of Physiological Genomics, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Vosberg S; Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; German Cancer Consortium, Partner site Munich, Munich 81377, Germany.; German Cancer Research Center, Heidelberg 69120, Germany.; Division of Oncology, Department of Internal Medicine, Medical University of Graz, Graz 8010, Austria., Bravo J; Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main 60596, Germany., Lutz S; Division of Transfusion Medicine, Cell Therapeutics and Haemostaseology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Kellner C; Division of Transfusion Medicine, Cell Therapeutics and Haemostaseology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Fischer A; Department of Internal Medicine III, University Hospital Regensburg, Regensburg 93053, Germany., Gebhard C; Leibniz Institute for Immunotherapy, Regensburg 93053, Germany., Redondo Monte E; Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; German Cancer Consortium, Partner site Munich, Munich 81377, Germany.; German Cancer Research Center, Heidelberg 69120, Germany., Hartmann L; Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; German Cancer Consortium, Partner site Munich, Munich 81377, Germany.; German Cancer Research Center, Heidelberg 69120, Germany., Schneider S; Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; Laboratory for Leukemia Diagnostics, Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; Institute of Human Genetics, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Beier F; Department of Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, Medical Faculty University Hospital Aachen, Rheinisch-Westfälische Technische Hochschule Aachen, Aachen 52074, Germany., Strobl CD; Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; German Cancer Consortium, Partner site Munich, Munich 81377, Germany.; German Cancer Research Center, Heidelberg 69120, Germany., Weigert O; Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; German Cancer Consortium, Partner site Munich, Munich 81377, Germany.; German Cancer Research Center, Heidelberg 69120, Germany., Peipp M; Division of Antibody-Based Immunotherapy, Department of Medicine II, Christian Albrechts University of Kiel, Kiel 24105, Germany., Schündeln M; Pediatric Hematology and Oncology, Department of Pediatrics III, University Hospital Essen and the University of Duisburg-Essen, Essen 45147, Germany., Stricker SH; Biomedical Center, Department of Physiological Genomics, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Rehli M; Department of Internal Medicine III, University Hospital Regensburg, Regensburg 93053, Germany.; Leibniz Institute for Immunotherapy, Regensburg 93053, Germany., Bernhagen J; Vascular Biology, Institute for Stroke and Dementia Research, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; Munich Cluster for Systems Neurology, Munich 81377, Germany., Humpe A; Division of Transfusion Medicine, Cell Therapeutics and Haemostaseology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany., Klump H; Institute for Transfusion Medicine, University Hospital Essen, Essen 45147, Germany.; Institute for Transfusion Medicine and Cell Therapeutics, University Hospital Aachen, Rheinisch-Westfälische Technische Hochschule Aachen, Aachen 52074, Germany., Brendel C; Division of Pediatric Hematology/Oncology, Boston Children's Hospital, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115., Krause DS; Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, Frankfurt am Main 60596, Germany.; Institute of General Pharmacology and Toxicology, University Hospital Frankfurt, Goethe-University, Frankfurt am Main 60596, Germany., Greif PA; Department of Medicine III, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.; German Cancer Consortium, Partner site Munich, Munich 81377, Germany.; German Cancer Research Center, Heidelberg 69120, Germany., Wichmann C; Division of Transfusion Medicine, Cell Therapeutics and Haemostaseology, University Hospital, Ludwig-Maximilians-Universität München, Munich 81377, Germany.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jun 18; Vol. 121 (25), pp. e2312499121. Date of Electronic Publication: 2024 Jun 10.

Folding makes an imprint.

Autor: Stricker SH; Reprogramming and Regeneration, Biomedical Center (BMC), Physiological Genomics, Faculty of Medicine, Ludwig Maximilian University (LMU) Munich, Planegg-Martinsried 82152, Germany; Epigenetic Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, Planegg-Martinsried 82152, Germany stefan.stricker@helmholtz-muenchen.de.

Publikováno v: Genes & development [Genes Dev] 2023 Sep 01; Vol. 37 (17-18), pp. 779-780. Date of Electronic Publication: 2023 Oct 11.

Innate Immune Pathways Promote Oligodendrocyte Progenitor Cell Recruitment to the Injury Site in Adult Zebrafish Brain.

Autor: Sanchez-Gonzalez R; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Department Biology II, University of Munich, 80539 München, Germany., Koupourtidou C; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Graduate School Systemic Neurosciences, LMU, 80539 Munich, Germany., Lepko T; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Graduate School Systemic Neurosciences, LMU, 80539 Munich, Germany., Zambusi A; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Graduate School Systemic Neurosciences, LMU, 80539 Munich, Germany., Novoselc KT; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Graduate School Systemic Neurosciences, LMU, 80539 Munich, Germany., Durovic T; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Graduate School Systemic Neurosciences, LMU, 80539 Munich, Germany., Aschenbroich S; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Graduate School Systemic Neurosciences, LMU, 80539 Munich, Germany., Schwarz V; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Graduate School Systemic Neurosciences, LMU, 80539 Munich, Germany., Breunig CT; Reprogramming and Regeneration, Biomedical Center (BMC), Physiological Genomics, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany., Straka H; Department Biology II, University of Munich, 80539 München, Germany., Huttner HB; Department of Neurology, Justus-Liebig-University Giessen, Klinikstrasse 33, 35392 Giessen, Germany., Irmler M; Institute of Experimental Genetics, Helmholtz Center Munich, 85764 Oberschleißheim, Germany., Beckers J; Institute of Experimental Genetics, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; German Center for Diabetes Research (DZD e.V.), 85764 Neuherberg, Germany.; Chair of Experimental Genetics, School of Life Sciences Weihenstephan, Technical University Munich, 80333 München, Germany., Wurst W; Institute of Developmental Genetics, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Munich Cluster for Systems Neurology SYNERGY, LMU, 80539 Munich, Germany.; Chair of Developmental Genetics c/o Helmholtz Zentrum München, School of Life Sciences Weihenstephan, Technical University Munich, 80333 München, Germany.; German Center for Neurodegenerative Diseases (DZNE), Site Munich, 80539 Munich, Germany., Zwergal A; Department of Neurology, Ludwig-Maximilians University, Campus Grosshadern, 81377 Munich, Germany., Schauer T; Biomedical Center (BMC), Bioinformatic Core Facility, Faculty of Medicine, LMU Munich, 80539 München, Germany., Straub T; Biomedical Center (BMC), Bioinformatic Core Facility, Faculty of Medicine, LMU Munich, 80539 München, Germany., Czopka T; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh EH8 9YL, UK., Trümbach D; Institute of Developmental Genetics, Helmholtz Center Munich, 85764 Oberschleißheim, Germany., Götz M; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Munich Cluster for Systems Neurology SYNERGY, LMU, 80539 Munich, Germany.; Biomedical Center (BMC), Division of Physiological Genomics, Faculty of Medicine, LMU Munich, 80539 München, Germany., Stricker SH; Reprogramming and Regeneration, Biomedical Center (BMC), Physiological Genomics, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany., Ninkovic J; Institute of Stem Cell Research, Helmholtz Center Munich, 85764 Oberschleißheim, Germany.; Biomedical Center (BMC), Division of Cell Biology and Anatomy, Faculty of Medicine, LMU Munich, 80539 München, Germany.; Munich Cluster for Systems Neurology SYNERGY, LMU, 80539 Munich, Germany.

Publikováno v: Cells [Cells] 2022 Feb 02; Vol. 11 (3). Date of Electronic Publication: 2022 Feb 02.

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Autor: Russo GL; Physiological Genomics, Biomedical Center (BMC), Ludwig-Maximilians-Universität (LMU), Planegg-Martinsried, Germany; Institute for Stem Cell Research, Helmholtz Center Munich, BMC LMU, Planegg-Martinsried, Germany; Graduate School of Systemic Neurosciences, BMC, LMU, Planegg-Martinsried, Germany., Sonsalla G; Physiological Genomics, Biomedical Center (BMC), Ludwig-Maximilians-Universität (LMU), Planegg-Martinsried, Germany; Institute for Stem Cell Research, Helmholtz Center Munich, BMC LMU, Planegg-Martinsried, Germany; Graduate School of Systemic Neurosciences, BMC, LMU, Planegg-Martinsried, Germany., Natarajan P; Physiological Genomics, Biomedical Center (BMC), Ludwig-Maximilians-Universität (LMU), Planegg-Martinsried, Germany; Institute for Stem Cell Research, Helmholtz Center Munich, BMC LMU, Planegg-Martinsried, Germany; Graduate School of Systemic Neurosciences, BMC, LMU, Planegg-Martinsried, Germany., Breunig CT; MCN Junior Research Group, Munich Center for Neurosciences, BMC, LMU, Planegg-Martinsried, Germany; Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, Planegg-Martinsried, Germany., Bulli G; Physiological Genomics, Biomedical Center (BMC), Ludwig-Maximilians-Universität (LMU), Planegg-Martinsried, Germany; Institute for Stem Cell Research, Helmholtz Center Munich, BMC LMU, Planegg-Martinsried, Germany., Merl-Pham J; Research Unit Protein Science, Helmholtz Center Munich, Neuherberg, Germany., Schmitt S; Institute of Toxicology and Environmental Hygiene, School of Medicine, Technical University Munich (TUM), Munich, Germany., Giehrl-Schwab J; Institute of Developmental Genetics, Helmholtz Center Munich, Neuherberg, Germany., Giesert F; Institute of Developmental Genetics, Helmholtz Center Munich, Neuherberg, Germany; Developmental Genetics, TUM, Munich-Weihenstephan, Germany., Jastroch M; Department of Molecular Biosciences, The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, Stockholm, Sweden., Zischka H; Institute of Toxicology and Environmental Hygiene, School of Medicine, Technical University Munich (TUM), Munich, Germany; Institute of Molecular Toxicology and Pharmacology, Helmholtz Center Munich, Neuherberg, Germany., Wurst W; Institute of Developmental Genetics, Helmholtz Center Munich, Neuherberg, Germany; Developmental Genetics, TUM, Munich-Weihenstephan, Germany; German Center for Neurodegenerative Diseases (DZNE) Site Munich, Munich, Germany., Stricker SH; MCN Junior Research Group, Munich Center for Neurosciences, BMC, LMU, Planegg-Martinsried, Germany; Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, Planegg-Martinsried, Germany., Hauck SM; Research Unit Protein Science, Helmholtz Center Munich, Neuherberg, Germany., Masserdotti G; Physiological Genomics, Biomedical Center (BMC), Ludwig-Maximilians-Universität (LMU), Planegg-Martinsried, Germany; Institute for Stem Cell Research, Helmholtz Center Munich, BMC LMU, Planegg-Martinsried, Germany., Götz M; Physiological Genomics, Biomedical Center (BMC), Ludwig-Maximilians-Universität (LMU), Planegg-Martinsried, Germany; Institute for Stem Cell Research, Helmholtz Center Munich, BMC LMU, Planegg-Martinsried, Germany; Excellence Cluster of Systems Neurology (SYNERGY), Munich, Germany. Electronic address: magdalena.goetz@helmholtz-muenchen.de.

Publikováno v: Cell stem cell [Cell Stem Cell] 2021 Mar 04; Vol. 28 (3), pp. 524-534.e7. Date of Electronic Publication: 2020 Nov 16.

CRISPR Tools for Physiology and Cell State Changes: Potential of Transcriptional Engineering and Epigenome Editing.

Autor: Breunig CT; MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian- Universität, BioMedical Center, Planegg-Martinsried, Germany; and Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, BioMedical Center, Planegg-Martinsried, Germany., Köferle A; MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian- Universität, BioMedical Center, Planegg-Martinsried, Germany; and Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, BioMedical Center, Planegg-Martinsried, Germany., Neuner AM; MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian- Universität, BioMedical Center, Planegg-Martinsried, Germany; and Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, BioMedical Center, Planegg-Martinsried, Germany., Wiesbeck MF; MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian- Universität, BioMedical Center, Planegg-Martinsried, Germany; and Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, BioMedical Center, Planegg-Martinsried, Germany., Baumann V; MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian- Universität, BioMedical Center, Planegg-Martinsried, Germany; and Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, BioMedical Center, Planegg-Martinsried, Germany., Stricker SH; MCN Junior Research Group, Munich Center for Neurosciences, Ludwig-Maximilian- Universität, BioMedical Center, Planegg-Martinsried, Germany; and Epigenetic Engineering, Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, BioMedical Center, Planegg-Martinsried, Germany.

Publikováno v: Physiological reviews [Physiol Rev] 2021 Jan 01; Vol. 101 (1), pp. 177-211. Date of Electronic Publication: 2020 Jun 11.