Genetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types.

Autor: Maggiore JC; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA., LeGraw R; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA., Przepiorski A; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA., Velazquez J; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA., Chaney C; Department of Molecular Biology and Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Streeter E; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA., Silva-Barbosa A; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh PA, 15213., Franks J; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Hislop J; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA., Hill A; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA., Wu H; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO 63130., Pfister K; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh PA, 15213., Howden SE; Murdoch Children's Research Institute, Melbourne, Victoria, Australia., Watkins SC; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Little M; Murdoch Children's Research Institute, Melbourne, Victoria, Australia.; Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne, Victoria, Australia.; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria, Australia., Humphreys BD; Division of Nephrology, Department of Medicine, School of Medicine, Washington University in St. Louis, St. Louis, MO 63130.; Department of Developmental Biology, School of Medicine, Washington University in St. Louis, St. Louis, MO 63130., Watson A; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Stolz DB; Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA 15213, USA., Kiani S; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA., Davidson AJ; Department of Molecular Medicine and Pathology, University of Auckland, Auckland 1010, New Zealand., Carroll TJ; Department of Molecular Biology and Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.; Department of Internal Medicine, Division of Nephrology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Cleaver O; Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX 75390., Sims-Lucas S; Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh PA, 15213., Ebrahimkhani MR; Department of Pathology, Division of Experimental Pathology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA., Hukriede NA; Department of Developmental Biology, School of Medicine, University of Pittsburgh, Pittsburgh PA 15213, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2023 May 30. Date of Electronic Publication: 2023 May 30.