A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues.

Autor: Glaser AK; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA. akglaser@uw.edu.; Allen Institute for Neural Dynamics, Seattle, WA, USA. akglaser@uw.edu., Bishop KW; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.; Department of Bioengineering, University of Washington, Seattle, WA, USA., Barner LA; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA., Susaki EA; Department of Biochemistry and Systems Biomedicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan., Kubota SI; Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.; Division of Molecular Psychoimmunology, Institute for Genetic Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan., Gao G; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA., Serafin RB; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA., Balaram P; Allen Institute for Brain Science, Seattle, WA, USA., Turschak E; Allen Institute for Brain Science, Seattle, WA, USA., Nicovich PR; Cajal Neuroscience, Seattle, WA, USA., Lai H; Leica Microsystems, Inc, Buffalo Grove, IL, USA., Lucas LAG; Leica Microsystems, Inc, Buffalo Grove, IL, USA., Yi Y; Department of Restorative Sciences, Texas A&M University, Dallas, TX, USA., Nichols EK; Department of Genome Sciences, University of Washington, Seattle, WA, USA., Huang H; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA., Reder NP; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA., Wilson JJ; Department of Immunology, University of Washington, Seattle, WA, USA., Sivakumar R; Department of Immunology, University of Washington, Seattle, WA, USA., Shamskhou E; Department of Immunology, University of Washington, Seattle, WA, USA., Stoltzfus CR; Department of Immunology, University of Washington, Seattle, WA, USA., Wei X; Department of Urology, University of Washington, Seattle, WA, USA., Hempton AK; Department of Biology, University of Washington, Seattle, WA, USA., Pende M; MDI Biological Laboratory, Bar Harbor, ME, USA., Murawala P; MDI Biological Laboratory, Bar Harbor, ME, USA.; Clinic for Kidney and Hypertension Diseases, Hannover Medical School, Hannover, Germany., Dodt HU; Department for Bioelectronics, Vienna University of Technology, Vienna, Austria.; Section for Bioelectronics, Center for Brain Research, Medical University of Vienna, Vienna, Austria., Imaizumi T; Department of Biology, University of Washington, Seattle, WA, USA., Shendure J; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Howard Hughes Medical Institute, Seattle, WA, USA.; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, USA.; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA., Beliveau BJ; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Brotman Baty Institute for Precision Medicine, Seattle, WA, USA., Gerner MY; Department of Immunology, University of Washington, Seattle, WA, USA., Xin L; Department of Urology, University of Washington, Seattle, WA, USA., Zhao H; Department of Restorative Sciences, Texas A&M University, Dallas, TX, USA., True LD; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA., Reid RC; Allen Institute for Brain Science, Seattle, WA, USA., Chandrashekar J; Allen Institute for Neural Dynamics, Seattle, WA, USA.; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Ueda HR; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan., Svoboda K; Allen Institute for Neural Dynamics, Seattle, WA, USA., Liu JTC; Department of Mechanical Engineering, University of Washington, Seattle, WA, USA. jonliu@uw.edu.; Department of Bioengineering, University of Washington, Seattle, WA, USA. jonliu@uw.edu.; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA. jonliu@uw.edu.

Publikováno v: Nature methods [Nat Methods] 2022 May; Vol. 19 (5), pp. 613-619. Date of Electronic Publication: 2022 May 11.

TGFβ restricts expansion, survival, and function of T cells within the tuberculous granuloma.

Autor: Gern BH; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA 98109, USA; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA., Adams KN; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA 98109, USA., Plumlee CR; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA 98109, USA., Stoltzfus CR; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Shehata L; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Moguche AO; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Busman-Sahay K; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA., Hansen SG; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA., Axthelm MK; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA., Picker LJ; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA., Estes JD; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA., Urdahl KB; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA 98109, USA; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA; Department of Immunology, University of Washington, Seattle, WA 98109, USA. Electronic address: kevin.urdahl@seattlechildrens.org., Gerner MY; Department of Immunology, University of Washington, Seattle, WA 98109, USA. Electronic address: gernermy@uw.edu.

Publikováno v: Cell host & microbe [Cell Host Microbe] 2021 Apr 14; Vol. 29 (4), pp. 594-606.e6. Date of Electronic Publication: 2021 Mar 11.

CytoMAP: A Spatial Analysis Toolbox Reveals Features of Myeloid Cell Organization in Lymphoid Tissues.

Autor: Stoltzfus CR; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Filipek J; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Gern BH; Seattle Children's Research Institute, Seattle, WA 98109, USA; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA., Olin BE; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Leal JM; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Wu Y; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Lyons-Cohen MR; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Huang JY; Department of Immunology, University of Washington, Seattle, WA 98109, USA., Paz-Stoltzfus CL; School of Medicine, University of Washington, Seattle, WA 98195, USA., Plumlee CR; Seattle Children's Research Institute, Seattle, WA 98109, USA., Pöschinger T; Roche Innovation Center Munich, Pharmaceutical Research & Early Development (pRED), Discovery Pharmacology, Nonnenwald 2, 82377 Penzberg, Germany., Urdahl KB; Department of Immunology, University of Washington, Seattle, WA 98109, USA; Seattle Children's Research Institute, Seattle, WA 98109, USA; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA., Perro M; Roche Innovation Center Zurich, Pharmaceutical Research & Early Development (pRED), Wagistrasse 10, 8952 Schlieren, Switzerland., Gerner MY; Department of Immunology, University of Washington, Seattle, WA 98109, USA. Electronic address: gernermy@uw.edu.

Publikováno v: Cell reports [Cell Rep] 2020 Apr 21; Vol. 31 (3), pp. 107523.

Optimizing ultrafast illumination for multiphoton-excited fluorescence imaging.

Autor: Stoltzfus CR; Physics Department, Montana State University, Bozeman MT 59717, USA., Rebane A; Physics Department, Montana State University, Bozeman MT 59717, USA; National Institute of Chemical Physics and Biophysics, Tallinn 12618, Estonia.

Publikováno v: Biomedical optics express [Biomed Opt Express] 2016 Apr 07; Vol. 7 (5), pp. 1768-82. Date of Electronic Publication: 2016 Apr 07 (Print Publication: 2016).

Two-photon directed evolution of green fluorescent proteins.

Autor: Stoltzfus CR; Physics Department, Montana State University, Bozeman MT. 59717., Barnett LM; Cell Biology Neuroscience Department, Montana State University, Bozeman MT. 59717., Drobizhev M; Physics Department, Montana State University, Bozeman MT. 59717., Wicks G; Physics Department, Montana State University, Bozeman MT. 59717., Mikhaylov A; Physics Department, Montana State University, Bozeman MT. 59717., Hughes TE; Cell Biology Neuroscience Department, Montana State University, Bozeman MT. 59717., Rebane A; 1] Physics Department, Montana State University, Bozeman MT. 59717 [2] National Institute of Chemical Physics and Biophysics, Tallinn, Estonia, 12618.

Publikováno v: Scientific reports [Sci Rep] 2015 Jul 06; Vol. 5, pp. 11968. Date of Electronic Publication: 2015 Jul 06.