Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Stilianos G. Roussis and"'
Publikováno v:
ACS Omega, Vol 9, Iss 48, Pp 47822-47830 (2024)
Externí odkaz:
https://doaj.org/article/5a4d1c04c5eb450bb858cddde3556759
Publikováno v:
Analytical Chemistry. 93:16035-16042
Replacement of a non-bridging oxygen atom of the phosphate diester linkage of an oligonucleotide by sulfur conveys pharmacokinetic benefits, such as increased nuclease resistance and enhanced protein binding. Substitution renders the internucleotide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7e50ced7516355726bdbd73f71eb9d7e
https://doi.org/10.1021/acs.analchem.1c03593
https://doi.org/10.1021/acs.analchem.1c03593
Autor:
Stilianos G. Roussis, Claus Rentel
Publikováno v:
Analytical biochemistry. 659
The separation of impurities in phosphorothioate diester (PS) oligonucleotides is complicated by (1) the presence of a very large number of diastereoisomers, e.g., 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::787e56c854607449669b85b4f10742e7
https://pubmed.ncbi.nlm.nih.gov/36270331
https://pubmed.ncbi.nlm.nih.gov/36270331
Autor:
Stilianos G. Roussis, Claus Rentel
Publikováno v:
Journal of Chromatography B. 1220:123666
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::30e44d431bd0d93f2b5f71e24fcdae3a
https://doi.org/10.1016/j.jchromb.2023.123666
https://doi.org/10.1016/j.jchromb.2023.123666
Publikováno v:
Rapid Communications in Mass Spectrometry. 33:1774-1780
Phosphorothioate oligonucleotide drugs typically contain product-related impurities that are difficult to resolve chromatographically from the parent oligonucleotide due to the size of these compounds and the large number of stereoisomers that compri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cb9e357170e00217b001998fad285ce
https://doi.org/10.1002/rcm.8516
https://doi.org/10.1002/rcm.8516
Autor:
Jonathan Chan, Stilianos G. Roussis, Jaimie DaCosta, Claus Rentel, Daniel C. Capaldi, Bao Mai
Publikováno v:
Journal of Pharmaceutical and Biomedical Analysis. 173:56-61
A novel analytical approach capable of measuring deaminated degradation products of oligonucleotide therapeutics is described. The method employs high-resolution mass spectrometry to assess the shift in isotopic distribution that accompanies deaminat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8173087ee61282200c1ae19d3a021adf
https://doi.org/10.1016/j.jpba.2019.05.012
https://doi.org/10.1016/j.jpba.2019.05.012
Publikováno v:
Rapid Communications in Mass Spectrometry. 32:1099-1106
Rationale Quantitative Ion-Pair (IP)-HPLC MS methods are employed to determine the complex impurity profiles of oligonucleotide therapeutics. While impurities that co-elute with the main product are routinely monitored, the large number of early and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::08ee0099f28c9d9e0003ca2698f03677
https://doi.org/10.1002/rcm.8125
https://doi.org/10.1002/rcm.8125
Publikováno v:
Journal of Chromatography B. 1169:122611
Ion pair-reversed phase (IP-RP) HPLC is one of the most widely used methods for the analysis of oligonucleotide impurities. The method is compatible with mass spectrometry and has been used to guide the development of improved synthesis and purificat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2466b416bc4f7ade5bbd0b8fb9feea55
https://doi.org/10.1016/j.jchromb.2021.122611
https://doi.org/10.1016/j.jchromb.2021.122611
Publikováno v:
Analytical Biochemistry. 595:113623
A new method is presented for the automated determination of early eluting (E.E.) oligonucleotide impurities analyzed by IP-RP HPLC HRMS. E.E. are impurities shorter than the main drug component and are primarily formed by the mechanisms of coupling
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d58636d4a66b8a27962b15aea6798d43
https://doi.org/10.1016/j.ab.2020.113623
https://doi.org/10.1016/j.ab.2020.113623
Publikováno v:
Journal of chromatography. A. 1594
In an effort to improve separation of impurities in oligonucleotide drugs, alkyl amines of different length and carbon content were evaluated as reagents in ion pair-reversed phase (IP-RP) HPLC with mass spectrometric detection. A range of columns wa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7b42755b4f589486456fc9f90eeff1a
https://pubmed.ncbi.nlm.nih.gov/30819437
https://pubmed.ncbi.nlm.nih.gov/30819437