Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Steven W. Sherwood"'
Publikováno v:
Molecular Immunology. 50:134-141
The objective of this study was to characterize the in vitro and in vivo activity of a novel afucosylated rituximab (BLX-300) expressed in a Lemna aquatic plant-based system free of zoonotic pathogens. The glycosylation of BLX-300 was shown to be hom
Autor:
W. Gregory Cox, Roderick A. Capaldi, Jakyoung Y. Agnew, Dani Hill, Steven W. Sherwood, Gayle Buller, Kunihiro Yamagata, Michael S. Janes, Bonnie J. Hanson
Publikováno v:
Journal of Histochemistry & Cytochemistry. 52:1011-1018
Nucleoside reverse transcriptase inhibitors (NRTIs) have been a mainstay in the treatment of human immunodeficiency virus since the introduction of azidothymidine (AZT) in 1987. However, none of the current therapies can completely eradicate the viru
Publikováno v:
Journal of Immunological Methods. 224:1-9
An assay based on two-color flow cytometry has been developed to measure CTL and NK cell-mediated cytotoxicity. After effector/target cells are incubated together, CTL or NK populations are stained with an effector cell specific PE-conjugated mAb. Su
Publikováno v:
Experimental Cell Research. 219:159-168
We have studied the effect of the cell synchronization agents compactin, ciclopirox olamine, mimosine, aphidicolin, ALLN, and colcemid on several parameters of cell cycle progression in mitotically synchronized HeLa S3 cells. Using cell size and cycl
Publikováno v:
Experimental Cell Research. 215:373-379
We have studied the relationship between apoptosis and drug-induced cell cycle perturbation in HeLa S3 cells when treated with the anti-tubulin drug colcemid. We found that at least two distinct mechanisms contributed to colcemid cytotoxicity and apo
Publikováno v:
Experimental Cell Research. 211:275-281
Using a procedure to stain cells simultaneously for cyclin B1 protein and DNA, we have examined cyclin B1 expression by flow cytometry in human cells under a variety of perturbing and nonperturbing conditions. The method described is useful for measu
Autor:
Catherine Carswell-Crumpton, Edward G. Spack, Electra C. Coucouvanis, Steven W. Sherwood, Patricia P. Jones
Publikováno v:
Experimental Cell Research. 209:238-247
Using fluorescence-activated cell sorting combined with fluorescence microscopy the mechanism of embryonic germ cell death in the mouse has been shown to be apoptosis. Primordial germ cells (PGCs) from embryos at specific developmental stages have be
Publikováno v:
Proceedings of the National Academy of Sciences. 87:9553-9557
This paper reports that there are major differences between mammalian cell lines in the propensity to progress into subsequent cell cycles when mitosis is inhibited with agents that disrupt the assembly of the mitotic spindle apparatus (Colcemid, noc
Autor:
Michael J. Mann, Ruediger C. Braun-Dullaeus, Heiko E. von der Leyen, Victor J. Dzau, Daniel Sedding, Steven W. Sherwood
Publikováno v:
Arteriosclerosis, thrombosis, and vascular biology. 24(5)
Objective— Although numerous diseases involving cellular proliferation are also associated with phenotypic changes, there has been little direct evidence that cell phenotype and the cell’s response to external stimuli are modified during passage