Structural basis for human mitochondrial tRNA maturation

Autor: Vincent Meynier, Steven W. Hardwick, Marjorie Catala, Johann J. Roske, Stephanie Oerum, Dimitri Y. Chirgadze, Pierre Barraud, Wyatt W. Yue, Ben F. Luisi, Carine Tisné

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)

Abstract The human mitochondrial genome is transcribed into two RNAs, containing mRNAs, rRNAs and tRNAs, all dedicated to produce essential proteins of the respiratory chain. The precise excision of tRNAs by the mitochondrial endoribonucleases (mt-RN

Externí odkaz: https://doaj.org/article/9225da7769584f1e885d93f4594d97f7

Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly

Autor: Steven W. Hardwick, Tobias Gubbey, Isabelle Hug, Urs Jenal, Ben F. Luisi

Publikováno v: Open Biology, Vol 2, Iss 4 (2012)

Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour. Its ring-like, trimeric architecture creates a central channel where phosphorolytic acti

Externí odkaz: https://doaj.org/article/b6a22234e53b4f1ebebf233bc94b888b

Two distinct long-range synaptic complexes promote different aspects of end processing prior to repair of DNA breaks by non-homologous end joining

Autor: Christopher J. Buehl, Noah J. Goff, Steven W. Hardwick, Martin Gellert, Tom L. Blundell, Wei Yang, Amanda K. Chaplin, Katheryn Meek

Publikováno v: Molecular Cell. 83:698-714.e4

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fd8108b4b7e39bb1b3f12e50aacf579c
https://doi.org/10.1016/j.molcel.2023.01.012

Single-particle cryo-EM at atomic resolution

Autor: Peter Tiemeijer, Abhay Kotecha, Erwin de Jong, Takanori Nakane, Dimitri Y. Chirgadze, D. Karia, Steven W. Hardwick, Andrija Sente, Maarten Bischoff, Lina Malinauskaite, Jamie McCormack, Garib N. Murshudov, S. Masiulis, A. Radu Aricescu, Patricia M. G. E. Brown, Tomasz Uchański, Jonas Miehling, Sjors H.W. Scheres, Lingbo Yu, Ioana T. Grigoras, Jeroen Keizer, Evgeniya V. Pechnikova, Greg McMullan, Tomas Malinauskas

Publikováno v: Nature

The three-dimensional positions of atoms in protein molecules define their structure and the roles they perform in biological processes. The more precisely atomic coordinates are determined, the more chemical information can be derived and the more m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c844e01171bd62488ea55ff1e98d24a
https://doi.org/10.1038/s41586-020-2829-0

Cryo-EM structures of staphylococcal IsdB bound to human hemoglobin reveal the process of heme extraction

Autor: Omar De Bei, Marialaura Marchetti, Luca Ronda, Eleonora Gianquinto, Loretta Lazzarato, Dimitri Y. Chirgadze, Steven W. Hardwick, Lee R. Cooper, Francesca Spyrakis, Ben F. Luisi, Barbara Campanini, Stefano Bettati

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America (Online) 119 (2022). doi:10.1073/pnas.2116708119
info:cnr-pdr/source/autori:De Bei O.; Marchetti M.; Ronda L.; Gianquinto E.; Lazzarato L.; Chirgadze D.Y.; Hardwick S.W.; Cooper L.R.; Spyrakis F.; Luisi B.F.; Campanini B.; Bettati S./titolo:Cryo-EM structures of staphylococcal IsdB bound to human hemoglobin reveal the process of heme extraction/doi:10.1073%2Fpnas.2116708119/rivista:Proceedings of the National Academy of Sciences of the United States of America (Online)/anno:2022/pagina_da:/pagina_a:/intervallo_pagine:/volume:119

Iron surface determinant B (IsdB) is a hemoglobin (Hb) receptor essential for hemic iron acquisition by Staphylococcus aureus. Heme transfer to IsdB is possible from oxidized Hb (metHb), but inefficient from Hb either bound to oxygen (oxyHb) or bound

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1d50f4dba0cd0e43687bebde65d7b65