Zobrazeno 1 - 10
of 60
pro vyhledávání: '"Steven Spiker"'
Autor:
Steven Spiker, Kirk E. Francis
Publikováno v:
The Plant Journal. 41:464-477
Several recent investigations of T-DNA integration sites in Arabidopsis thaliana have reported 'cold spots' of integration, especially near centromeric regions. These observations have contributed to the ongoing debate over whether T-DNA integration
Publikováno v:
Transgenic Research. 12:3-12
We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression
Autor:
Mara Massel, William F. Thompson, Jennifer L. Ingram, Steven Spiker, Arthur K. Weissinger, Robert Ascenzi
Publikováno v:
Transgenic Research. 10:465-470
The tobacco nuclear matrix attachment region (MAR), RB7, has been shown to have a much greater effect on transgene expression in cultured cells than in transgenic plants. This is comparable to work in mouse systems showing that MARs have a positive e
Publikováno v:
Plant Molecular Biology. 43:361-376
Matrix attachment regions (MARs) are operationally defined as DNA elements that bind specifically to the nuclear matrix in vitro. It is possible, although unproven, that they also mediate binding of chromatin to the nuclear matrix in vivo and alter t
Publikováno v:
Biochemistry. 38:12795-12804
Matrix attachment regions (MARs) can be operationally defined as DNA fragments that bind to the nuclear matrix. We have created a library of randomly obtained MARs from tobacco (Nicotiana tobacum) by cloning DNA fragments that co-isolate with nuclear
Publikováno v:
The Plant Journal. 18:253-263
Summary The RB7 matrix attachment region (MAR), when flanking a uidA (GUS) reporter gene, has been previously shown to increase uidA gene expression by 60-fold in stably transformed tobacco suspension cell lines. We have now used the same co-transfor
Publikováno v:
Journal of Biological Chemistry. 271:25742-25745
Previous work indicated that nuclear extracts isolated from embryogenic rice suspension cells treated with the phytohormone abscisic acid (ABA) have enhanced binding activity to an ABA response element (Em1a) in the promoter of the Em gene from wheat
Autor:
Steven Spiker, Young-Doo Park, István Papp, Antonius J. M. Matzke, Susan Michalowski, Marjori Matzke, Victor A. Iglesias, Eduardo A. Moscone
Publikováno v:
The Plant Journal. 10:469-478
This paper describes molecular and cytogenetic evidence for the stability of a transgene locus that is present on the triplicated chromosome in an aneuploid tobacco line. This instability was manifested in several ways in trisomics including a major
Publikováno v:
The Plant Journal. 6:413-423
The β-phaseolin promoter yields high levels of spatially and temporally regulated expression in transgenic plants. It is shown that elements within 5′ and 3′ regions flanking the coding sequence bind strongly and specifically to matrix preparati
Publikováno v:
Transgenic Research. 11:311-313
In our studies of the factors that regulate the expression of foreign genes (transgenes) introduced into plant genomes, we routinely use microprojectile bombardment (Allen et al., 2000). This approach has found widespread application in agronomically