Zobrazeno 1 - 10
of 134
pro vyhledávání: '"Steven Ripp"'
Publikováno v:
BioTechniques, Vol 71, Iss 2, Pp 403-415 (2021)
Due to the public health concerns of endocrine-disrupting chemicals, there is an increasing demand to develop improved high-throughput detection assays for enhanced exposure control and risk assessment. A substrate-free, autobioluminescent HEK293ARE/
Externí odkaz:
https://doaj.org/article/f346d6ceb9b747cbbfff2427ad909bfc
Publikováno v:
Frontiers in Microbiology, Vol 13 (2022)
Transport of pathogenic bacteria from land surface to groundwater is largely influenced by rainfall intensity and geochemical and structural heterogeneities of subsurface sediments at different depths. It has been assumed that the change in rainfall
Externí odkaz:
https://doaj.org/article/50e4422a588d47878f7489d0d50c33e2
Publikováno v:
BMC Biology, Vol 18, Iss 1, Pp 1-14 (2020)
Abstract Background Luminescent reporter proteins are vital tools for visualizing cells and cellular activity. Among the current toolbox of bioluminescent systems, only bacterial luciferase has genetically defined luciferase and luciferin synthesis p
Externí odkaz:
https://doaj.org/article/758317ebdf3d413db0b6402cf9feaf62
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
Technologies enabling on-site environmental detection or medical diagnostics in resource-limited settings have a strong disruptive potential compared to current analytical approaches that require trained personnel in laboratories with immobile, resou
Externí odkaz:
https://doaj.org/article/f62a2c4fa14f46159732a7c6fb641d49
Autor:
Liqiong Yang, Xijuan Chen, Xiangfeng Zeng, Mark Radosevich, Steven Ripp, Jie Zhuang, Gary S. Sayler
Publikováno v:
Frontiers in Microbiology, Vol 10 (2019)
Chemotaxis and haptotaxis are important biological mechanisms that influence microbial movement toward concentrated chemoattractants in mobile liquids and along immobile surfaces, respectively. This study investigated their coupled effect, as induced
Externí odkaz:
https://doaj.org/article/fe14037b5b9c46ad9a97f7d45c16484f
Publikováno v:
Sensors, Vol 20, Iss 11, p 3237 (2020)
In this study, we show the repetitive detection of toluene on a tapered optical fiber element (OFE) with an attached layer of Pseudomonas putida TVA8 bioluminescent bioreporters. The bioluminescent cell layer was attached on polished quartz modified
Externí odkaz:
https://doaj.org/article/e3ae5441e4b542f3ab6e491f23e00866
Publikováno v:
Sensors, Vol 12, Iss 2, Pp 1544-1571 (2012)
Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first g
Externí odkaz:
https://doaj.org/article/f3f0e44410b448408685db8f2ab211cc
Autor:
Gary Sayler, Steven Ripp, Sarah Price, Robert Crossley, Alexandra Rogers, Abby Smartt, Tingting Xu, Dan Close
Publikováno v:
Sensors, Vol 12, Iss 1, Pp 732-752 (2012)
The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the
Externí odkaz:
https://doaj.org/article/36080a0e3f754199a04f38393916f462
Publikováno v:
Sensors, Vol 11, Iss 1, Pp 180-206 (2010)
In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter ce
Externí odkaz:
https://doaj.org/article/3ac11535fec345ad91a3e59e6e4cee50
Publikováno v:
Sensors, Vol 17, Iss 12, p 2827 (2017)
Modern drug discovery workflows require assay systems capable of replicating the complex interactions of multiple tissue types, but that can still function under high throughput conditions. In this work, we evaluate the use of substrate-free autobiol
Externí odkaz:
https://doaj.org/article/184eaadd1c85410a90afae8955cc230b