First quantitative high-throughput screen in zebrafish identifies novel pathways for increasing pancreatic β-cell mass

Autor: Guangliang Wang, Surendra K Rajpurohit, Fabien Delaspre, Steven L Walker, David T White, Alexis Ceasrine, Rejji Kuruvilla, Ruo-jing Li, Joong S Shim, Jun O Liu, Michael J Parsons, Jeff S Mumm

Publikováno v: eLife, Vol 4 (2015)

Whole-organism chemical screening can circumvent bottlenecks that impede drug discovery. However, in vivo screens have not attained throughput capacities possible with in vitro assays. We therefore developed a method enabling in vivo high-throughput

Externí odkaz: https://doaj.org/article/6bc5a75f96814fc3aa7ccaecb3f5d1b1

Automated reporter quantification in vivo: high-throughput screening method for reporter-based assays in zebrafish.

Autor: Steven L Walker, Junko Ariga, Jonathan R Mathias, Veena Coothankandaswamy, Xiayang Xie, Martin Distel, Reinhard W Köster, Michael J Parsons, Kapil N Bhalla, Meera T Saxena, Jeff S Mumm

Publikováno v: PLoS ONE, Vol 7, Iss 1, p e29916 (2012)

Reporter-based assays underlie many high-throughput screening (HTS) platforms, but most are limited to in vitro applications. Here, we report a simple whole-organism HTS method for quantifying changes in reporter intensity in individual zebrafish ove

Externí odkaz: https://doaj.org/article/cb453aee935a411d96f8838e1cb39404

Mutations in CHD7, Encoding a Chromatin-Remodeling Protein, Cause Idiopathic Hypogonadotropic Hypogonadism and Kallmann Syndrome

Autor: Ingo Kurth, Gil Bu Kang, Metin Ozata, Irene Meliciani, Mustafa Tekin, Richard J. Sherins, Yang Shi, Wolfgang Wenzel, Georg Rosenberger, Fei Lan, Soo Hyun Eom, Lawrence C. Layman, James F. Gusella, David P. Bick, Hyung Goo Kim, Steven L. Walker

Publikováno v: The American Journal of Human Genetics. 83:511-519

CHARGE syndrome and Kallmann syndrome (KS) are two distinct developmental disorders sharing overlapping features of impaired olfaction and hypogonadism. KS is a genetically heterogeneous disorder consisting of idiopathic hypogonadotropic hypogonadism

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e9b25bc657c0e5c7adca53935d9db8e
https://doi.org/10.1016/j.ajhg.2008.09.005

First quantitative high-throughput screen in zebrafish identifies novel pathways for increasing pancreatic β-cell mass

Autor: Steven L. Walker, Alexis M Ceasrine, Rejji Kuruvilla, Michael J. Parsons, Fabien Delaspre, Joong Sup Shim, Jun O. Liu, Ruo-Jing Li, Surendra Kumar Rajpurohit, David T. White, Jeff S. Mumm, Guangliang Wang

Publikováno v: Recercat: Dipósit de la Recerca de Catalunya
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
eLife
Repositorio Abierto de la UdL
Universitad de Lleida
Recercat. Dipósit de la Recerca de Catalunya
instname
eLife, Vol 4 (2015)

Whole-organism chemical screening can circumvent bottlenecks that impede drug discovery. However, in vivo screens have not attained throughput capacities possible with in vitro assays. We therefore developed a method enabling in vivo high-throughput

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::762c9bb3ca60704d7b6488f080c992c6
https://doi.org/10.7554/elife.08261

Evaluating human cancer cell metastasis in zebrafish

Autor: Steven L. Walker, David T. White, Yong Teng, Jeff S. Mumm, John K. Cowell, Xiayang Xie

Publikováno v: BMC Cancer

Background In vivo metastasis assays have traditionally been performed in mice, but the process is inefficient and costly. However, since zebrafish do not develop an adaptive immune system until 14 days post-fertilization, human cancer cells can surv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6813343d59fc94192802123ce7dfc87
https://pubmed.ncbi.nlm.nih.gov/24089705