Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Steven L. Broitman"'
Autor:
Benjamin Stevens, Chunlai Chen, Ian Farrell, Haibo Zhang, Jaskiran Kaur, Steven L Broitman, Zeev Smilansky, Barry S Cooperman, Yale E Goldman
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e38344 (2012)
We present proof-of-concept in vitro results demonstrating the feasibility of using single molecule fluorescence resonance energy transfer (smFRET) measurements to distinguish, in real time, between individual ribosomes programmed with several differ
Externí odkaz:
https://doaj.org/article/f49773a75a654027816c8f47173d3619
Autor:
Chunlai Chen, Michael Reiche, Steven L. Broitman, Ian Farrell, Yale E. Goldman, Haibo Zhang, Barry S. Cooperman
Publikováno v:
Nature Structural & Molecular Biology
Nature structural & molecular biology
Nature structural & molecular biology
During protein synthesis, the ribosome translates nucleotide triplets in single-stranded mRNA into polypeptide sequences. Strong downstream mRNA secondary structures, which must be unfolded for translation, can slow or even halt protein synthesis. He
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79cdf28e32a76eaf8c69f25b6dfa1358
Publikováno v:
Nucleic Acids Research. 31:4673-4681
Three identical deoxyoligonucleotide third strands with a 3'-terminal psoralen moiety attached by linkers that differ in length (N = 16, 6 and 4 atoms) and structure were examined for their ability to form triplex-directed psoralen photoproducts with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2babfb65a9d9723f6c8171370b940e1
https://doi.org/10.1093/nar/gkg659
https://doi.org/10.1093/nar/gkg659
Publikováno v:
Journal of Biological Chemistry. 274:21763-21768
A DNA third strand with a 3′-psoralen substituent was designed to form a triplex with the sequence downstream of the T·A mutant base pair of the human sickle cell β-globin gene. Triplex-mediated psoralen modification of the mutant T residue was s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1b9a45689603d45ee047f71f265284ed
https://doi.org/10.1074/jbc.274.31.21763
https://doi.org/10.1074/jbc.274.31.21763
Autor:
Steven L. Broitman
Publikováno v:
Progress in Biophysics and Molecular Biology. 63:119-129
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8b44caa39de8185a5ee6a32be3cb952
https://doi.org/10.1016/0079-6107(95)00001-4
https://doi.org/10.1016/0079-6107(95)00001-4
Publikováno v:
Biochemical and Biophysical Research Communications. 189:1674-1680
We have identified a purine-rich triplex binding sequence overlapping a −35 transcriptional early promoter region of the bacteriophage T 7 . Triplex-forming oligonucleotide designed to bind this target was annealed to T 7 templates and introduced i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d30df6cf83563715ed7b6a1f8ce1ce3
https://doi.org/10.1016/0006-291x(92)90270-u
https://doi.org/10.1016/0006-291x(92)90270-u
Autor:
Chunlai Chen, Jaskiran Kaur, Yale E. Goldman, Barry S. Cooperman, Haibo Zhang, Zeev Smilansky, Benjamin Stevens, Ian Farrell, Steven L. Broitman
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e38344 (2012)
PLoS ONE
PLoS ONE
We present proof-of-concept in vitro results demonstrating the feasibility of using single molecule fluorescence resonance energy transfer (smFRET) measurements to distinguish, in real time, between individual ribosomes programmed with several differ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0432b4c3c7a4f9bca14e7a0faba422f4
https://doi.org/10.1371/journal.pone.0038344
https://doi.org/10.1371/journal.pone.0038344
Publikováno v:
Proceedings of the National Academy of Sciences. 84:5120-5124
A polynucleotide helical structure containing two strands of poly(A) and one of poly(U) is reported. As shown by spectroscopic observations, the complex only forms when the poly(A) strands are of Mr between 9000 and 50,000 (degree of polymerization c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27c6e7c324d9ee9ce3bf4636c857574d
https://doi.org/10.1073/pnas.84.15.5120
https://doi.org/10.1073/pnas.84.15.5120
Using ultraviolet spectral techniques, the hydrogen bonding schemes have been determined for the A·C and I·U (as analogue of G·T) base mispairs in heli-ces dominated by the Watson-Crick pairs A·U or I·C. Each mispair contains one of its bases in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a0d4908f3e868da8c1a44bb480d5a1c2
https://doi.org/10.1016/b978-0-12-048850-6.50069-7
https://doi.org/10.1016/b978-0-12-048850-6.50069-7
Autor:
Barry S. Cooperman, Chunlai Chen, Yale E. Goldman, Steven L. Broitman, Ian Farrell, Haibo Zhang
Publikováno v:
Biophysical Journal. (3):67a-68a
During protein synthesis, the ribosome moves along a single mRNA strand and translates the genetic code into polypeptide sequences. Strong mRNA secondary structures, which have to be unfolded in order to be translated, can slow or even halt protein s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abb140d4dac38f4d280169f49e50a7ee