Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Steven K. Koester"'
Publikováno v:
Cytometry. 33:324-332
A breast tumor hypoxia model used to simulate conditions which may exist within an enlarging tumor was examined using documented methods for identifying mechanisms of cell death and compared to the mitochondrial membrane-specific APO2.7 antigen expre
Autor:
Steven K. Koester, Wade E. Bolton
Publikováno v:
Clinical Immunology Newsletter. 18:97-102
Autor:
Patricia Roth, Chonghui Zhang, Stuart F. Schlossman, Steven K. Koester, Warren R. Mikulka, Wade E. Bolton
Publikováno v:
Cytometry. 29:306-312
A recently described mitochondrial membrane protein-specific monoclonal antibody, APO2.7, was examined for monitoring early apoptotic responses in anti-CD95 (7C11)-induced Jurkat cells. Jurkat cells were harvested at 1.5, 3, 4.5, 6, 12, and 18 h afte
Autor:
Steven K. Koester, Melissa C. O'Brien, Barry Avner, Shula R. Raney, Stephen F. Healy, Josephine M. Hurst, Andrew Hanly, James W. Freeman, Carolyn Mies, Wade E. Bolton, Christopher Snow
Publikováno v:
Cytometry. 28:81-89
A method is described for the discrimination of Type III, late apoptotic, and necrotic cells, to improve the accuracy of proliferation and ploidy determinations of breast tumors. We selected an immunological probe, antitubulin antibody, and a DNA spe
Autor:
Barbara Y. Ho, Courtney M. Townsend, Pamella J. Ford, Steven K. Koester, S. C. Barranco, Karen J. Reumont
Publikováno v:
Investigational New Drugs. 8:S9-S18
A clone of human gastric cancer cells (AGS-6) and the parental line (AGS-P) from which it was isolated were used in cell survival studies to determine whether pretreatment for 24, 48 or 72h with alpha-difluoromethylornithine (DFMO, 5mM) would increas
Autor:
Steven K. Koester, Wade E. Bolton
Publikováno v:
Cytometry ISBN: 9780125441667
Publisher Summary This chapter discusses a logical approach for the permeabilization and fixation of cells such that surface and intracellular antigens can be preserved and quantified. It is understood that no single fixative or permeabilization reag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::956b36743dc2c15526bd96156c9dfe9c
https://doi.org/10.1016/s0091-679x(01)63016-4
https://doi.org/10.1016/s0091-679x(01)63016-4
Autor:
Steven K. Koester, Wade E. Bolton
Publikováno v:
Cytometry ISBN: 9780125441667
Publisher Summary Caspases are initially found as inactive proteins called zymogens and must be cleaved to become active effectors in the death cascade. Active cleaved products can now specifically cleave other zymogens to create a cascade of enzymat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d9389d9fa302633bc0272d465612f240
https://doi.org/10.1016/s0091-679x(01)63026-7
https://doi.org/10.1016/s0091-679x(01)63026-7
Autor:
Steven K. Koester, Wade E. Bolton
Publikováno v:
Journal of immunological methods. 243(1-2)
An increased level of complexity will be encountered when developing protocols for intracellular markers. Protocols for surface markers have been successfully standardized, however it is understood that no single method is appropriate for all intrace
Autor:
Wade E. Bolton, Steven K. Koester
Publikováno v:
Clinical chemistry and laboratory medicine. 37(3)
Three documented cell death pathways, apoptosis, necrosis, and oncosis will be discussed. The end result of each pathway is cell death; however, the path by which death is achieved and the morphological and physiological traits of each may be strikin
Autor:
Jan M. Woynarowski, Cheryl Napier, John R. MacDonald, William G. Chapman, Shih-Fong Chen, Steven K. Koester, Dean A. Troyer
Publikováno v:
Biochemical pharmacology. 54(11)
6-Hydroxymethylacylfulvene (HMAF, MGI 114) is a new alkylating antitumor sesquiterpenoid with promising and often curative antitumor activity in vivo. This study examined the ability of the drug to damage cellular DNA, induce apoptosis, and affect th