Genome editing reveals that pSCL4 is required for chromosome linearity in Streptomyces clavuligerus

Autor: Andrew J. Collis, Juan Pablo Gomez-Escribano, Kenan A. J. Bozhüyük, Paul Herron, Jason Micklefield, Barrie Wilkinson, Benjamin D. Huckle, Lis Algora Gallardo, Steven G. Kendrew, Mervyn J. Bibb, Nicola A. Crowhurst

Publikováno v: Gomez-Escribano, J P, Algora Gallardo, L, Bozhüyük, K A J, Kendrew, S G, Huckle, B D, Crowhurst, N A, Bibb, M J, Collis, A J, Micklefield, J, Herron, P R & Wilkinson, B 2021, ' Genome editing reveals that pSCL4 is required for chromosome linearity in Streptomyces clavuligerus ', Microbial genomics, vol. 7, no. 11, 000669 . https://doi.org/10.1099/MGEN.0.000669

Streptomyces clavuligerus is an industrially important actinomycete whose genetic manipulation is limited by low transformation and conjugation efficiencies, low levels of recombination of introduced DNA, and difficulty in obtaining consistent sporul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aaf44edb3f28327a8a9c1d6553fdb4b2
https://www.research.manchester.ac.uk/portal/en/publications/genome-editing-reveals-that-pscl4-is-required-for-chromosome-linearity-in-streptomyces-clavuligerus(37af815e-dc6e-4e8b-850d-a1893497f85d).html

Cloning, Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of C-12 Hydroxylase EryK from Saccharopolyspora erythraea

Autor: Steven G. Kendrew, Giuliano Sciara, Adriana E. Miele, Carmelinda Savino, Beatrice Vallone

Publikováno v: Protein and Peptide Letters
Protein and Peptide Letters, Bentham Science Publishers, 2008, 15 (10), pp.1138-1141. ⟨10.2174/092986608786071201⟩
Protein and peptide letters
15 (2008): 1138–1141. doi:10.2174/092986608786071201
info:cnr-pdr/source/autori:Savino,C.; Miele,A.E.; Sciara,G.; Kendrew,S.G.; Vallone,B./titolo:Cloning, epression, purification, crystallization and preliminary X-ray crystallographic analyis of C-12 Hydroxylase EryK from Saccharoplyspora erythraea/doi:10.2174%2F092986608786071201/rivista:Protein and peptide letters (Print)/anno:2008/pagina_da:1138/pagina_a:1141/intervallo_pagine:1138–1141/volume:15

Erythromycin A is produced by Saccharopolyspora erythraea via a secondary metabolic pathway using several steps including glycosylations and hydroxylations of the first macrolide intermediate 6-deoxyerythronolide B. Erythromycin C-12 hydroxylase (CYP

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::620647e22ba6b56c47b902357dff914f
https://doi.org/10.2174/092986608786071201

An Aromatic Hydroxylation Reaction Catalyzed by a Two-component FMN-dependent Monooxygenase

Autor: Marc Fontecave, Vincent Nivière, Julien Valton, Steven G. Kendrew, Thierry Douki

Publikováno v: Journal of Biological Chemistry. 281:27-35

The ActVA-ActVB system from Streptomyces coelicolor isatwo-component flavin-dependent monooxygenase that belongs to an emerging class of enzymes involved in various oxidation reactions in microorganisms. The ActVB component is a NADH:flavin oxidoredu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::74d651d07c0b8ce61a36b7ef520784cf
https://doi.org/10.1074/jbc.m506146200

Active-site residue, domain and module swaps in modular polyketide synthases

Autor: Peter F. Leadlay, J. Staunton, Hrvoje Petković, Steven G. Kendrew, Brian A.M. Rudd, Lindsey Low, Rachel E. Lill, Jesus Cortes, Francesca Del Vecchio, Barrie Wilkinson

Publikováno v: Journal of Industrial Microbiology and Biotechnology. 30:489-494

Sequence comparisons of multiple acyltransferase (AT) domains from modular polyketide synthases (PKSs) have highlighted a correlation between a short sequence motif and the nature of the extender unit selected. When this motif was specifically altere

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97ed0bc787c420862b87659cbb528633
https://doi.org/10.1007/s10295-003-0062-0

[Untitled]

Autor: Cheon-Seok Park, C. R. Hutchinson, Jonathan Kennedy, Karine Auclair, John C. Vederas, Steven G. Kendrew

Publikováno v: Antonie van Leeuwenhoek. 78:287-295

Lovastatin biosynthesis in Aspergillus terreus involves two unusual type I multifunctional polyketide syntheses (PKSs). Lovastatin nonaketide synthase (LNKS), the product of the lovB gene, is an iterative PKS that interacts with LovC, a putative enoy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a383275383d2094524c4736e3a356e19
https://doi.org/10.1023/a:1010294330190