Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Steven C L Ho"'
Autor:
Esther Y C Koh, Steven C L Ho, Mariati, Zhiwei Song, Xuezhi Bi, Muriel Bardor, Yuansheng Yang
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e82100 (2013)
A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons of 10(th), 11(th), and 12(th) AUG to non-AUG triplets. They are able to
Externí odkaz:
https://doaj.org/article/32f5b977ccbd4f19ad7f48c227b8aa08
Autor:
Steven C L Ho, Muriel Bardor, Bin Li, Jia Juan Lee, Zhiwei Song, Yen Wah Tong, Lin-Tang Goh, Yuansheng Yang
Publikováno v:
PLoS ONE, Vol 8, Iss 5, p e63247 (2013)
Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal ribosome entry site (IRES) and furin-2A (F2A) for their influence on
Externí odkaz:
https://doaj.org/article/061242949654436eaaecd8c51b9d3a9f
Publikováno v:
Molecular biotechnology. 59(8)
Role of epigenetic regulation in the control of gene expression is well established. The impact of several epigenetic mechanisms, such as DNA methylation and histone acetylation, on recombinant protein production in mammalian cells has been investiga
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1126a162db3892bdae4324c5882cb018
https://pubmed.ncbi.nlm.nih.gov/28664508
https://pubmed.ncbi.nlm.nih.gov/28664508
Autor:
Shu Hui Neo, Yuansheng Yang, Jessna H. M. Yeo, Steven C. L. Ho, Tianhua Wang, Xuezhi Bi, Jian'er Lin, Sheng-Hao Chao, Wei Zhang
Publikováno v:
Biotechnology journal. 12(9)
Studies had shown the benefits of using furin-2A peptides for high monoclonal antibody (mAb) expression in mammalian cells. How signal peptides affect furin-2A mediated mAb secretion has yet to be investigated. The impact of signal peptides on mAb se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::832d4b5f2f597dc44882d6fd9f7ac69b
https://pubmed.ncbi.nlm.nih.gov/28727292
https://pubmed.ncbi.nlm.nih.gov/28727292
Publikováno v:
Bioengineered. 5:340-345
Maintaining high gene expression level during long-term culture is critical when producing therapeutic recombinant proteins using mammalian cells. Transcriptional silencing of promoters, most likely due to epigenetic events such as DNA methylation an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c801fe30d6bf8c352660df2205ddd02a
https://doi.org/10.4161/bioe.32111
https://doi.org/10.4161/bioe.32111
Publikováno v:
Journal of bioscience and bioengineering. 122(4)
Plant and yeast derived hydrolysates are economical and efficient alternative medium supplements to improve mammalian cell culture performance. We supplemented two commercial Chinese hamster ovary (CHO) culture media with hydrolysates from four diffe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0843f39403d9da0594e3391090c9194d
https://pubmed.ncbi.nlm.nih.gov/27067279
https://pubmed.ncbi.nlm.nih.gov/27067279
Publikováno v:
Protein Expression and Purification. 69:9-15
Five post-transcriptional regulatory elements, (i) the 50 untranslated region (UTR) of human heat shock protein 70 mRNA (Hsp70), (ii) the 163-bp long splice variant derived from the 50 UTR of vascular endothelial growth factor (SP163), and (iii) the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd70753013018a5d828817d6c320dbbf
https://doi.org/10.1016/j.pep.2009.08.010
https://doi.org/10.1016/j.pep.2009.08.010
Publikováno v:
Biotechnology and Bioengineering. 102:1152-1160
A set of mutated SV40 early polyadenylation signals (SV40pA) with varying strengths is generated by mutating the AATAAA sequence in the wild-type SV40pA. They are shown to control the expression level of a gene over a 10-fold range using luciferase r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a3ce5abca7347ab8591a78ebdaef356
https://doi.org/10.1002/bit.22152
https://doi.org/10.1002/bit.22152
Autor:
Benjamin P. C. Soo, Mariati, Yuansheng Yang, Steven C. L. Ho, Sheng-Hao Chao, Esther Y. C. Koh
Publikováno v:
BMC Biotechnology
Background Methylated CpG dinucleotides in promoters are associated with the loss of gene expression in recombinant Chinese hamster ovary (CHO) cells during large-scale commercial manufacturing. We evaluated a promoter devoid of CpG dinucleotides, Cp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0adf43f216f2fa57e7c621816141bbbf
https://pubmed.ncbi.nlm.nih.gov/27756290
https://pubmed.ncbi.nlm.nih.gov/27756290
Autor:
Rui Nian, Ally Lau, Jake Chng, Kong Meng Hoi, Yuansheng Yang, Xuezhi Bi, Steven C. L. Ho, Pete Gagnon, Tianhua Wang
Publikováno v:
mAbs. 7(2)
Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio and reduces non-expressing cells. Four 2A peptides derived from the f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e709e5bd6f06a528529f86cea774d3ab
https://pubmed.ncbi.nlm.nih.gov/25621616
https://pubmed.ncbi.nlm.nih.gov/25621616