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Akademický článek

Sufficiency of hypoxia-inducible 2-oxoglutarate dioxygenases to block chemical oxidative stress-induced differentiation of human embryonic stem cells

Autor: Eirini Koutsouraki, Steve Pells, Paul A. De Sousa

Publikováno v: Stem Cell Research, Vol 34, Iss , Pp - (2019)

Hypoxia benefits undifferentiated pluripotent stem cell renewal, and 2-oxoglutarate (2OG) dioxygenases have been implicated in pluripotent stem cell induction and renewal. We show in human embryonic stem cells (hESC) that an ambient oxygen-induced ox

Externí odkaz: https://doaj.org/article/1749dabbe1414cdb8cd41963262ece05

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Akademický článek

Novel Human Embryonic Stem Cell Regulators Identified by Conserved and Distinct CpG Island Methylation State.

Autor: Steve Pells, Eirini Koutsouraki, Sofia Morfopoulou, Sara Valencia-Cadavid, Simon R Tomlinson, Ravi Kalathur, Matthias E Futschik, Paul A De Sousa

Publikováno v: PLoS ONE, Vol 10, Iss 7, p e0131102 (2015)

Human embryonic stem cells (hESCs) undergo epigenetic changes in vitro which may compromise function, so an epigenetic pluripotency "signature" would be invaluable for line validation. We assessed Cytosine-phosphate-Guanine Island (CGI) methylation i

Externí odkaz: https://doaj.org/article/a23f5da6431c4b93b37c2f45b9627544

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A scalable label-free approach to separate human pluripotent cells from differentiated derivatives

Autor: Paz Freile, P.A. De Sousa, Steve Pells, G. McPhee, C. Williams, Debaditya Choudhury, Henry Bock, Marieke A. Hoeve, Nicholas Willoughby

Publikováno v: Willoughby, N A, Bock, H, Hoeve, M A, Pells, S, Williams, C, Mcphee, G, Freile, P, Choudhury, D & De Sousa, P A 2016, ' A scalable label-free approach to separate human pluripotent cells from differentiated derivatives ', Biomicrofluidics, vol. 10, no. 1, pp. 014107 . https://doi.org/10.1063/1.4939946

The broad capacity of pluripotent human embryonic stem cells (hESC) to grow and differentiate demands the development of rapid, scalable, and label-free methods to separate living cell populations for clinical and industrial applications. Here, we id

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2392724d876cf6f1ea32cd32429941a
https://hdl.handle.net/20.500.11820/16ab0fa3-3858-4be4-80eb-a884ff5b2430

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Paracrine signalling events in embryonic stem cell renewal mediated by affinity targeted nanoparticles

Autor: Paz Freile, Pierre Bagnaninchi, Jason Park, Tarek M. Fahmy, Steve Pells, Bruna Corradetti, Paul A. De Sousa

Publikováno v: Corradetti, B, Freile, P, Pells, S, Bagnaninchi, P, Park, J, Fahmy, T M & de Sousa, P A 2012, ' Paracrine signalling events in embryonic stem cell renewal mediated by affinity targeted nanoparticles ', Biomaterials, vol. 33, no. 28, pp. 6634-6643 . https://doi.org/10.1016/j.biomaterials.2012.06.011
Biomaterials
Biomaterials; Vol 33

Stem cell growth and differentiation is controlled by intrinsic and extrinsic factors. The latter includes growth factors, which are conventionally supplied in vitro in media exchanged daily. Here, we illustrate the use of affinity targeted biodegrad

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c80c0cfdfef75e427887b1610e36e63
https://doi.org/10.1016/j.biomaterials.2012.06.011

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Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

Autor: Stewart Smith, Colin Chung, Martin Waterfall, Anoop Menachery, Steve Pells, Ronald Pethig

Publikováno v: Journal of Electrical Bioimpedance, Vol 2, Iss 1, Pp 64-71 (2011)

Dielectrophoresis (DEP) is a label-free technique for the characterization and manipulation of biological particles - such as cells, bacteria and viruses. Many studies have focused on the DEP cross-over frequency f xo1, where cells in a non-uniform e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d945912fd6d84820bfc9a85d2705469c
https://www.journals.uio.no/index.php/bioimpedance/article/view/196

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Lineage-specific distribution of high levels of genomic

Autor: Yanina Tsenkina, David C. Hay, Andrea Serio, Ian Wilmut, Mark Bradley, Steve Pells, Zara Hannoun, Judy Fletcher, Siddharthan Chandran, Alexey Ruzov, Tatiana Dudnakova, Paul A. De Sousa, Gareth J. Sullivan, Tatiana Chebotareva, Yu Bai

Publikováno v: Ruzov, A, Tsenkina, Y, Serio, A, Dudnakova, T, Fletcher, J, Bai, Y, Chebotareva, T, Pells, S, Hannoun, Z, Sullivan, G, Chandran, S, Hay, D C, Bradley, M, Wilmut, I & De Sousa, P 2011, ' Lineage-specific distribution of high levels of genomic 5-hydroxymethylcytosine in mammalian development ', Cell Research, vol. 21, no. 9, pp. 1332-1342 . https://doi.org/10.1038/cr.2011.113
Cell Research

Methylation of cytosine is a DNA modification associated with gene repression. Recently, a novel cytosine modification, 5-hydroxymethylcytosine (5-hmC) has been discovered. Here we examine 5-hmC distribution during mammalian development and in cellul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdee6a4f63cfcb743034c1fa3fdcf4a9
https://doi.org/10.1038/cr.2011.113

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Human embryonic stem cells rapidly take up and then clear exogenous human and animal prionsin vitro

Autor: Jean Manson, Enrico Cancellotti, G. Robin Barclay, Kay Samuel, Paul A. De Sousa, Matthew Bishop, Steve Pells, Mark Head, Zuzana Krejciova, James W. Ironside, Marc Turner, Paz Freile

Publikováno v: The Journal of Pathology. 223:635-645

Susceptibility to prion infection involves interplay between the prion strain and host genetics, but expression of the host-encoded cellular prion protein is a known prerequisite. Here we consider human embryonic stem cell (hESC) susceptibility by ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3db238f4dfe3fd5e4a9053b90e37211d
https://doi.org/10.1002/path.2832

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Clinically failed eggs as a source of normal human embryo stem cells

Autor: Daniel R. Brison, Ian Wilmut, K. John McLaughlin, D.M. Collins, Britt Jorgensen Tye, Steve Pells, Susan J. Kimber, Sharon Sneddon, John Gardner, Paul A. De Sousa, Karen Stewart, Pawlina Dand, Stefan Przyborski, Brian A. Lieberman, Michael J. Cooke, Lisa Shaw

Publikováno v: Stem Cell Research. 2:188-197

The promise of human embryo stem cells (hESCs) for regenerative medicine is offset by the ethical and practical challenges involved in sourcing eggs and embryos for this objective. In this study we sought to isolate an hESC line from clinically faile

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5947020b4a108f3cf3d80aed91a855c6
https://doi.org/10.1016/j.scr.2009.01.002

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Increased bone mass, altered trabecular architecture and modified growth plate organization in the growing skeleton of SOCS2 deficient mice

Autor: Steve Pells, Vicky E MacRae, Syed Faisal Ahmed, Andrew A. Pitsillides, R S Collinson, Colin Farquharson, H Dale, S Horvat

Publikováno v: MacRae, V, Horvat, S, Pells, S C, Dale, H, Collinson, R S, Pitsillides, A A, Ahmed, S F & Farquharson, C 2009, ' Increased bone mass, altered trabecular architecture and modified growth plate organization in the growing skeleton of SOCS2 deficient mice ', Journal of Cellular Physiology, vol. 218, no. 2, pp. 276-84 . https://doi.org/10.1002/jcp.21593

Suppressor of cytokine signalling-2 (SOCS2) negatively regulates the signal transduction of several cytokines. Socs2(-/-) mice show increased longitudinal skeletal growth associated with deregulated GH/IGF-1 signalling. The present study examined the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6dbcf5e7071d829f19dfded7001689e
https://doi.org/10.1002/jcp.21593

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