Výsledky vyhledávání - "Steve F. Parsons"

Akademický článek

Induction of adult levels of β-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL

Autor: Kongtana Trakarnsanga, Marieangela C. Wilson, Winnie Lau, Belinda K. Singleton, Steve F. Parsons, Punthita Sakuntanaga, Ryo Kurita, Yukio Nakamura, David J. Anstee, Jan Frayne

Publikováno v: Haematologica, Vol 99, Iss 11 (2014)

A major barrier to the clinical use of erythrocytes generated in vitro from pluripotent stem cells or cord blood progenitors is failure of these erythrocytes to express adult hemoglobin. The key regulators of globin switching KLF1 and BCL11A are abse

Externí odkaz: https://doaj.org/article/fa9914930d7a4284b64ccca182c292dc

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Akademický článek

Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS.

Autor: Kongtana Trakarnsanga, Marieangela C Wilson, Rebecca E Griffiths, Ashley M Toye, Lee Carpenter, Kate J Heesom, Steve F Parsons, David J Anstee, Jan Frayne

Publikováno v: PLoS ONE, Vol 9, Iss 7, p e100874 (2014)

Induced pluripotent stem cells (iPSC) are an attractive progenitor source for the generation of in vitro blood products. However, before iPSC-derived erythroid cells can be considered for therapeutic use their similarity to adult erythroid cells must

Externí odkaz: https://doaj.org/article/a5c97a43397a4584ad42b90d3800431e

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Comparison of the proteome of adult and cord erythroid cells, and changes in the proteome following reticulocyte maturation

Autor: David J. Anstee, Marieangela C. Wilson, Nicola Cogan, Steve F. Parsons, Kongtana Trakarnsanga, Jan Frayne, Carole Green, Ashley M. Toye, Kate J. Heesom

Publikováno v: Wilson, M, Trakarnsanga, K, Heesom, K, Toye, A & Frayne, J 2016, ' Comparison of the proteome of adult and cord erythroid cells, and changes in the proteome following reticulocyte maturation ', Molecular and Cellular Proteomics, vol. 15, no. 6, pp. 1938-1946 . https://doi.org/10.1074/mcp.M115.057315

Cord blood stem cells are an attractive starting source for the production of red blood cells in vitro for therapy because of additional expansion potential compared with adult peripheral blood progenitors and cord blood banks usually being more repr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c547dbbf9a8800f561c9e719e8e8ceb
https://hdl.handle.net/1983/b273f2a3-b8e1-462d-8f78-de4e5a905735

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Mutations in the second zinc finger of human EKLF reduce promoter affinity but give rise to benign and disease phenotypes

Autor: Jan Frayne, Marieangela C. Wilson, Winnie W. Y. Lau, R. Leo Brady, Ben M. Richardson, Nicholas M. Burton, David J. Anstee, Belinda K. Singleton, Victoria Fairweather, Steve F. Parsons, Kongtana Trakarnsanga

Publikováno v: Blood. 118:3137-3145

Mutations in the human erythroid Krüppel-like factor (EKLF) can lead to either anemia or the benign InLu phenotype. To elucidate the relationship between these mutations and the differing phenotypes, we prepared recombinant forms of wild-type and 5

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::721d555261ad062caa2df772ce2abb86
https://doi.org/10.1182/blood-2011-04-349985

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Qualitative and Quantitative Comparison of the Proteome of Erythroid Cells Differentiated from Human iPSCs and Adult Erythroid Cells by Multiplex TMT Labelling and NanoLC-MS/MS

Autor: Jan Frayne, Rebecca E. Griffiths, David J. Anstee, Marieangela C. Wilson, Kate J. Heesom, Ashley M. Toye, Steve F. Parsons, Kongtana Trakarnsanga, Lee Carpenter

Publikováno v: PLoS ONE
PLoS ONE, Vol 9, Iss 7, p e100874 (2014)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99fe7a8d6114d5183223ec14e9d68178
http://europepmc.org/articles/PMC4096399

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Analysis of the differential proteome of human erythroblasts during in vitro erythropoiesis by 2-D DIGE

Autor: Jan Frayne, Ben M. Richardson, Steve F. Parsons, Kate J. Heesom, Dave J. Anstee

Publikováno v: Proteomics. Clinical applications. 3(9)

In the present study we have used an in vitro culture system that induces differentiation of human CD34(+) cells down the erythroid lineage along with 2-D DIGE to determine the differential proteome of erythroblasts at specific developmental stages d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6053d7ad9164183ede6c1627e7825762
https://pubmed.ncbi.nlm.nih.gov/21137011

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A Robust Ex Vivo culture System for Production of Mature Human Reticulocytes From a Single Donor

Autor: David J. Anstee, Steve F. Parsons, Nicola Cogan, Sabine Kupzig

Publikováno v: Blood. 118:2096-2096

Abstract 2096 We have formulated a robust culture system simulating human erythropoiesis, producing mature reticulocytes from adult peripheral blood CD34+ progenitor cells in 20 days. Expansion of cell numbers over the course of the culture exceeded

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::76b9d667460a03a9cfb731b30394af12
https://doi.org/10.1182/blood.v118.21.2096.2096

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Plasma Membrane Loss in Maturing Human Reticulocytes Occurs Through Endocytosis of Large Glycophorin A- Containing Vacuoles Which Fuse with Autophagosomes Before Exocytosis

Autor: Jon D. Lane, Virginie M S Betin, Rebecca E. Griffiths, Steve F. Parsons, David J. Anstee, Nicola Cogan, Sabine Kupzig, Tosti J. Mankelow

Publikováno v: Blood. 118:177-177

Abstract 177 The erythrocyte is one of the best characterized human cells. However, studies of the process whereby human reticulocytes mature to erythrocytes have been hampered by the difficulty of obtaining sufficient numbers of cells for analysis.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b0d9a28189da4bf4afd7619ebefc1335
https://doi.org/10.1182/blood.v118.21.177.177

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