Is space the final frontier for mitochondrial study?

Autor: Neikirk K; Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA., Stephens DC; Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA.; Department of Life & Physical Sciences, Fisk University, Nashville, TN 37208, USA., Beasley HK; Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA., Marshall AG; Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA., Gaddy JA; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Damo SM; Department of Life & Physical Sciences, Fisk University, Nashville, TN 37208, USA., Hinton A Jr; Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA.

Publikováno v: BioTechniques [Biotechniques] 2024 Feb; Vol. 76 (2), pp. 46-51. Date of Electronic Publication: 2023 Dec 12.

Three-dimensional mitochondria reconstructions of murine cardiac muscle changes in size across aging.

Autor: Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Garza-Lopez E; Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States., Christensen TA; Microscopy and Cell Analysis Core Facility, Mayo Clinic, Rochester, Minnesota, United States., Shao J; Central Microscopy Research Facility, University of Iowa, Iowa City, Iowa, United States., Lam J; Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Anudokem J Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Rodriguez B; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Kirk B; Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States., Bacevac S; Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States., Barongan T; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Shao B; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States.; Department of Life and Physical Sciences, Fisk University, Nashville, Tennessee, United States., Kabugi K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Koh HJ; Department of Biological Sciences, Tennessee State University, Nashville, Tennessee, United States., Koh A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Evans CS; Department of Cell Biology, Duke University School of Medicine, Durham, North Carolina, United States., Taylor B; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, United States., Reddy AK; Department of Medicine, Baylor College of Medicine, Houston, Texas, United States., Miller-Fleming T; Division of Genetic Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States., Actkins KV; Division of Genetic Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States., Zaganjor E; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States., Daneshgar N; Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States., Murray SA; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States., Mobley BC; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States., Damo SM; Department of Life and Physical Sciences, Fisk University, Nashville, Tennessee, United States., Gaddy JA; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, United States.; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States.; Tennessee Valley Healthcare Systems, United States Department of Veterans Affairs, Nashville, Tennessee, United States., Riggs B; Department of Biology at San Francisco State University, San Francisco, California, United States., Wanjalla C; Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States., Kirabo A; Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States., McReynolds M; Department of Biochemistry and Molecular Biology, Pennsylvania State University, State College, Pennsylvania, United States., Gomez JA; Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States., Phillips MA; Department of Integrative Biology, Oregon State University, Corvallis, Oregon, United States., Exil V; Division of Cardiology, Department of Pediatrics, St. Louis University School of Medicine, St. Louis, Missouri, United States.; Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States., Dai DF; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States., Hinton A Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States.

Publikováno v: American journal of physiology. Heart and circulatory physiology [Am J Physiol Heart Circ Physiol] 2023 Nov 01; Vol. 325 (5), pp. H965-H982. Date of Electronic Publication: 2023 Aug 25.

Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells.

Autor: Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Garza-Lopez E; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Mungai M; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue N; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Turner K; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Shao J; Central Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA., Murray S; Department of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, PA, 15260, USA., Gaddy JA; Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232 USA.; Tennessee Valley Healthcare Systems, U.S. Department of Veterans Affairs, Nashville, Tennessee, 37212 USA., Wanjalla C; Vanderbilt University Medical Center: Department of Medicine, Division of Infectious Disease, Nashville, TN, USA., Davis J; Department of Biochemistry and Cancer Biology. Meharry Medical College, Nashville, TN, 37208, USA., Damo S; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Hinton AO; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2023 Aug 19. Date of Electronic Publication: 2023 Aug 19.

Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation.

Autor: Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Mungai M; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Garza-Lopez E; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue N; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Turner K; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Shao JQ; Central Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA., Sarker B; School of Applied Computational Sciences, Meharry Medical College, Nashville, TN, 37232, USA., Murray S; Department of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, TN, 15260, USA., Gaddy JA; Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.; Tennessee Valley Healthcare Systems, U.S. Department of Veterans Affairs, Nashville, Tennessee, USA., Davis J; Department of Biochemistry and Cancer Biology. Meharry Medical College, Nashville, TN, USA., Damo SM; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA., Hinton AO Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2023 Aug 19. Date of Electronic Publication: 2023 Aug 19.

Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology.

Autor: Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Scudese E; Laboratory of Biosciences of Human Motricity (LABIMH) of the Federal University of State of Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil.; Sport Sciences and Exercise Laboratory (LaCEE), Catholic University of Petrópolis (UCP), Catholic, 25685-100, Brazil., Lopez EG; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Shao B; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Krystofiak E; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, 37232, USA., Rutledge S; Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL, 35899, USA., Davis J; Department of Biochemistry, Cancer Biology, Neuroscience, Pharmacology, Meharry Medical College, Nashville, TN, 37232, USA., Murray SA; Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, 15261, USA., Damo SM; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37208, USA.; Center for Structural Biology, Vanderbilt University, Nashville, TN, 37232, USA., Katti P; National Heart, Lung and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, 20892, USA., Hinton A Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.

Publikováno v: Advanced biology [Adv Biol (Weinh)] 2023 Aug; Vol. 7 (8), pp. e2300139. Date of Electronic Publication: 2023 May 28.