Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Stephen P. Eisenberg"'
Autor:
Amber Haynes Fradkin, Stephen P. Eisenberg, Mary S. Rosendahl, Carl S. Boand, Theodore W. Randolph
Publikováno v:
Biotechnology Progress. 26:743-749
We expressed recombinant murine growth hormone (rmGH) in E. coli as a cost-effective way to produce large quantities (gram scale) of the protein for use in murine studies of immunogenicity to therapeutic proteins. High hydrostatic pressure was used t
Autor:
Elizabeth A. Chlipala, Kurt Christensen, Mary S. Rosendahl, Daniel H. Doherty, Dean P. Edwards, George N. Cox, Dana L. Long, Darin J. Smith, Stephen P. Eisenberg
Publikováno v:
Experimental Hematology. 34:697-704
Objective Erythropoietin (Epo) bioactivity is significantly reduced by modification of lysine residues with amine-reactive reagents, which are the most commonly used reagents for attaching polyethylene glycols (PEGs) to proteins to improve protein ha
Autor:
George N. Cox, Christine M. Fam, Mary S. Rosendahl, Elizabeth A. Chlipala, Sharon J. Carlson, Stephen P. Eisenberg
Publikováno v:
Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 34(10)
Interferon gamma (IFN-γ) is a 28 kDa homodimeric cytokine that exhibits potent immunomodulatory, anti-proliferative, and antiviral properties. The protein is used to treat chronic granulomatous disease and malignant osteopetrosis, and it is under in
Autor:
Stephen P. Eisenberg, S. Kaye Spratt, Alan P. Wolffe, Andrew C. Jamieson, Matthew C. Mendel, Pei-Xiang Li, Qiang Liu, Yuxin Liang, Lei Zhang, Bingliang Chen, Xiaohong Zhong, Pei-Qi Liu, Hong Qi, Casey C. Case, Edward J. Rebar, Ya-Li Lee
Publikováno v:
Journal of Biological Chemistry. 276:11323-11334
We have mapped conserved regions of enhanced DNase I accessibility within the endogenous chromosomal locus of vascular endothelial growth factor A (VEGF-A). Synthetic zinc finger protein (ZFP) transcription factors were designed to target DNA sequenc
Autor:
Stephen P. Eisenberg, Sharon M. Wahl, T B McNeely, Diane C. Shugars, Mary S. Rosendahl, Christina Tucker
Publikováno v:
Blood. 90:1141-1149
Infection of monocytes with human immunodeficiency virus type 1Ba-L (HIV-1Ba-L ) is significantly inhibited by treatment with the serine protease inhibitor, secretory leukocyte protease inhibitor (SLPI). SLPI does not appear to act on virus directly,
Autor:
Elizabeth A. Chlipala, Ji I Lee, Stephen P. Eisenberg, Mary S. Rosendahl, Jacquelyn D. Brown, George N. Cox, Daniel H. Doherty
Publikováno v:
Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 33(12)
Interferon beta (IFN-β) is widely used to ameliorate disease progression in patients with Multiple Sclerosis. IFN-β has a short half-life in humans, necessitating frequent administration for optimum effectiveness. Covalent modification of IFN-β wi
Autor:
Stephen P. Eisenberg, Robert C. Thompson, Jack J. Skalicky, Barbara J. Brandhuber, Guy Vigers, Jeff Bray, Ronald Evans, John D. Childs
Publikováno v:
Journal of Biological Chemistry. 270:11477-11483
Interleukin-1 receptor antagonist (IL-1ra), an IL-1 family member, binds with high affinity to the type I IL-1 receptor (IL-1RI), blocking IL-1 binding but not inducing an IL-1-like response. Extensive site-directed mutagenesis has been used to ident
Autor:
Stephen P. Eisenberg, Theresa T. Pizarro, Cynthia C. Nast, Maurizio Ferretti, Vincenzo Casini-Raggi, Fabio Cominelli
Publikováno v:
Journal of Clinical Investigation. 94:449-453
Administration of exogenous interleukin-1 receptor antagonist (IL-1ra) is effective in reducing the severity of disease in animal models of acute inflammation. However, the function of endogenous IL-1ra in this process, is not yet known. We investiga
Autor:
Ronald Evans, Barbara J. Brandhuber, Guy Vigers, Robert C. Thompson, Stephen P. Eisenberg, P. Caffes
Publikováno v:
Journal of Biological Chemistry. 269:12874-12879
Interleukin-1 receptor antagonist (IL-1ra) is a natural competitive antagonist of IL-1. In order to further elucidate the mechanism by which IL-1ra binds without activating the IL-1 receptor, we have solved the crystal structure of IL-1ra at 2.0-A re
Autor:
Maurizio Ferretti, Laura Monsacchi, Marina Bortolami, Fabio Cominelli, Theresa T. Pizarro, Ray K. Ng, Michael T Brewer, Stephen P. Eisenberg
Publikováno v:
Journal of Biological Chemistry. 269:6962-6971
Genomic and cDNA clones for rabbit interleukin-1 receptor antagonist (IL-1ra) were isolate based on homology with the human, mouse, and rat IL-1ra gene. A partial genomic clone, obtained by screening a rabbit genomic library, contained coding sequenc