Procedures for Flow Cytometry‐Based Sorting of Unfixed Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) Infected Cells and Other Infectious Agents

Autor: Evan R. Jellison, Geoffrey Lyon, Simon Monard, Benjamin Fontes, Jan Baijer, David R. Ambrozak, Kristen M. Reifel, Brandon K. Swan, Stephen P. Perfetto, Richard Nguyen

Publikováno v: Cytometry Part A
Cytometry

In response to the recent COVID‐19 pandemic, many laboratories are involved in research supporting SARS‐CoV‐2 vaccine development and clinical trials. Flow cytometry laboratories will be responsible for a large part of this effort by sorting un

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fff36062279b0acc1588df36cf0d204
https://doi.org/10.1002/cyto.a.24040

Methodology for evaluating and comparing flow cytometers: A multisite study of 23 instruments

Autor: Faysal El Khettabi, John P. Nolan, Danilo Condello, James C. S. Wood, Ryan R. Brinkman, Josef Spidlen, Wayne A. Moore, Doug Redelman, Yong Chen, Jonathan Van Dyke, Lili Wang, Stephen P. Perfetto, David R. Parks

Publikováno v: Cytometry Part A. 93:1087-1091

We demonstrate improved methods for making valid and accurate comparisons of fluorescence measurement capabilities among instruments tested at different sites and times. We designed a suite of measurements and automated data processing methods to obt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d75b893c895276d48e787ef1165aa795
https://doi.org/10.1002/cyto.a.23605

Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2018 Conference Workshops

Autor: Kamila Czechowska, Giacomo Vacca, Cherie Green, Ruth M Barnard, Jaroslav Icha, Michael Nathan Hedrick, Gelo Victoriano Dela Cruz, Nicole E. Paul, Judith Arcidiacono, Andrew Filby, Jakob Zimmermann, Jonni S. Moore, Steven R. Bauer, Ruben Props, Jakub Nedbal, Yongliang Sun, Soren Ulrik Sonder, Christopher Hall, Caryn van Vreden, Cláudia Bispo, Paul K. Wallace, Rachel J. Errington, Jenny Molloy, Joanne Lannigan, Frederik Hammes, Johanna Ivaska, Thomas M. Ashhurst, Frederiek-Maarten Kerckhof, Michael Lee, Hyun-Dong Chang, Christian Kukat, Attila Tárnok, Nao Nitta, Robert S. Hoffman, Gert Van Isterdael, Lina Chakrabarti, John Sharpe, Michael Weber, Raluca Niesner, Christopher Groves, Peter Rubbens, Samson Rogers, Yanli Liu, Dominic Gagnon, Alessandra Vitaliti, Radhika Rayanki, Matthias Schiemann, Lili Wang, Robert Salomon, Grace Chojnowski, Rui Gardner, Bunny Cotleur, Derek H. Jones, John T. Elliott, Betsy Ohlsson-Wilhelm, Stefan Radtke, Maciej Cabanski, Ryan R. Brinkman, Michael Gregory, Henning Ulrich, Jennifer J. Stewart, Sheng Lin-Gibson, Dominic C. Jenner, Heba A. Degheidy, Virginia Litwin, Ziv Portat, Silas J. Leavesley, David Lanham, Susann Müller, Stephen P. Perfetto, Steven Eck, Aja M. Rieger, Diana L. Bonilla

Publikováno v: Cytometry A
Czechowska, K, Lannigan, J, Wang, L, Arcidiacono, J, Ashhurst, T M, Barnard, R M, Bauer, S R, Bispo, C, Bonilla, D L, Brinkman, R R, Cabanski, M, Chang, H-D, Chakrabarti, L, Chojnowski, G, Cotleur, B, Degheidy, H, Dela Cruz, G V, Eck, S, Elliott, J, Errington, R, Filby, A, Gagnon, D, Gardner, R, Green, C, Gregory, M, Groves, C J, Hall, C, Hammes, F, Hedrick, M, Hoffman, R O, Icha, J, Ivaska, J, Jenner, D C, Jones, D, Kerckhof, F M, Kukat, C, Lanham, D, Leavesley, S, Lee, M, Lin-Gibson, S, Litwin, V, Liu, Y, Molloy, J, Moore, J S, Müller, S, Nedbal, J, Niesner, R, Nitta, N, Ohlsson-Wilhelm, B, Paul, N E, Perfetto, S, Porat, Z, Props, R, Radtke, S, Rayanki, R, Rieger, A, Rogers, S, Rubbens, P, Salomon, R, Schiemann, M, Sharpe, J, Sonder, S U, Stewart, J J, Sun, Y, Ulrich, H, Van Isterdael, G, Vitaliti, A, van Vreden, C, Weber, M, Zimmermann, J, Vacca, G, Wallace, P & Tárnok, A 2019, ' Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2018 Conference Workshops ', Cytometry Part A, vol. 95, no. 6, pp. 598-644 . https://doi.org/10.1002/cyto.a.23777

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::813c17049ba93a16648134b42fb793b6
https://hdl.handle.net/10453/137837

Q and B values are critical measurements required for inter-instrument standardization and development of multicolor flow cytometry staining panels

Autor: James C. S. Wood, Pratip K. Chattopadhyay, Richard Nguyen, Mario Roederer, David R. Ambrozak, Stephen P. Perfetto, Juliane P. Hill

Publikováno v: Cytometry Part A. 85:1037-1048

Much of the complexity of multicolor flow cytometry experiments lies within the development of antibody staining panels and the standardization of instruments. In this article, we propose a theoretical metric and describe how measurements of sensitiv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::24847354e6cd65eaaa6cea3d30fea6fa
https://doi.org/10.1002/cyto.a.22579

High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer

Autor: Anu Puri, Masaki Terabe, Peter L. Choyke, Carley D. Ross, Jay A. Berzofsky, Aparna H. Kesarwala, James C. S. Wood, Jane B. Trepel, Stephen P. Perfetto, Joshua A. Welsh, John Tigges, Hisataka Kobayashi, William G. Telford, Marjorie Robert-Guroff, Alan Dean, Thorsten Demberg, Jennifer Jones, Bryant C. Yung, David R. Parks, Aliaksander Kachynski, Jonathan Van Dyke, Xiaoyuan Chen, Marty Bigos, Ariel Rosner, Thomas Musich, Gordon J. Freeman, Aizea Morales-Kastresana, Susan J. Knox, Edward J. Felton, Lise Pasquet, W. Roy Overton, Vasilis Toxavidis, Peter Guion, Katherine McKinnon, Veena Kapoor, Ionita Ghiran

Publikováno v: Journal of Extracellular Vesicles
Journal of extracellular vesicles, vol 8, iss 1
Journal of Extracellular Vesicles, Vol 8, Iss 1 (2019)

Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7b37c35b084de708e93d07459cc6c84
https://doi.org/10.1080/20013078.2019.1597603