Stabilizing additives added during cell lysis aid in the solubilization of recombinant proteins.

Autor: David J Leibly, Trang Nhu Nguyen, Louis T Kao, Stephen N Hewitt, Lynn K Barrett, Wesley C Van Voorhis

Publikováno v: PLoS ONE, Vol 7, Iss 12, p e52482 (2012)

Insoluble recombinant proteins are a major issue for both structural genomics and enzymology research. Greater than 30% of recombinant proteins expressed in Escherichia coli (E. coli) appear to be insoluble. The prevailing view is that insolubly expr

Externí odkaz: https://doaj.org/article/52b799caa70f41e0bc3c51827a86c114

Synthesis and biological evaluation of pyrazolopyrimidines as potential antibacterial agents

Autor: Stephen N. Hewitt, Darren W. Begley, R. Meganathan, Hannah S. Udell, Gashaw M. Goshu, Phillip G. Pierce, Peter J. Myler, Joy M. Bain, Debarati Ghose, Timothy J. Hagen

Publikováno v: Bioorganic & Medicinal Chemistry Letters. 25:5699-5704

The fragment FOL7185 (compound 17) was found to be a hit against IspD and IspE enzymes isolated from bacteria, and a series of analogs containing the pyrazolopyrimidine core were synthesized. The majority of these compounds inhibited the growth of Bu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3aafb3677fc49ebac81975bdeb46c84
https://doi.org/10.1016/j.bmcl.2015.10.096

Backbone chemical shift assignments for the sensor domain of the Burkholderia pseudomallei histidine kinase RisS: 'missing' resonances at the dimer interface

Autor: Garry W. Buchko, Thomas E. Edwards, Isabelle Phan, Wesley C. Van Voorhis, Samuel I. Miller, Peter J. Myler, Stephen N. Hewitt

Publikováno v: Biomolecular NMR Assignments. 9:381-385

Using a deuterated sample, all the observable backbone (1)H(N), (15)N, (13)C(a), and (13)C' chemical shifts for the dimeric, periplasmic sensor domain of the Burkholderia pseudomallei histidine kinase RisS were assigned. Approximately one-fifth of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3286b5c26cf0ef7ccddfd711ff44dad0
https://doi.org/10.1007/s12104-015-9614-2

Structure of a CutA1 divalent-cation tolerance protein fromCryptosporidium parvum, the protozoal parasite responsible for cryptosporidiosis

Autor: Yanfeng Zhang, Thomas E. Edwards, Jan Abendroth, Stephen N. Hewitt, Matthew C. Clifton, Garry W. Buchko, Wesley C. Van Voorhis, Howard Robinson, Bart L. Staker, Peter J. Myler

Publikováno v: Acta Crystallographica Section F Structural Biology Communications. 71:522-530

Cryptosporidiosis is an infectious disease caused by protozoan parasites of theCryptosporidiumgenus. Infection is associated with mild to severe diarrhea that usually resolves spontaneously in healthy human adults, but may lead to severe complication

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d280ebdb97e0293f0d285206d4b4c32c
https://doi.org/10.1107/s2053230x14028210

Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond

Autor: Christopher D. Huston, Amornrat Naranuntarat Jensen, Louis Maes, Jordi Mestres, Nao Aki Watanabe, Michael Adsetts Edberg Hansen, Roberto Adelfio, Simon Townson, Didier Leroy, Kate Weatherby, Thomas Spangenberg, Manuela Carrasquilla, Kailash P. Patra, Robert E. Sinden, José Brea, Abhai K. Tripathi, W. Armand Guiguemde, Alan Y. Du, Melanie Wree, Katherine T. Andrews, Kirsten K. Hanson, Tyler B. Hughes, Sundari Suresh, Adele M. Lehane, Sangeeta N. Bhatia, Edward J. Wojcik, Andrew Hemphill, Francielly Morais Rodrigues da Costa, Worathad Chindaudomsate, Joseph M. Vinetz, Ben Gold, Sunyoung Kim, Edgar Vigil, Nuha R. Mansour, Mohamed Abdo Rizk, Patrick Valere Tsouh Fokou, Audrey Burton, Laran T. Jensen, David A. Fidock, Aishah Alsibaee, Filipe Silva Villela, Yesmalie Alemán Resto, Rajarshi Guha, Conor R. Caffrey, José A. Fernández Robledo, Thomas J. Ketas, Luke Mercer, Rob Hooft van Huijsduijnen, Maria Jose Lafuente, Wesley C. Van Voorhis, Lauve R. Y. Tchokouaha, Dee A. Carter, Anjo Theron, Benoît Laleu, Kiaran Kirk, Maurice A. Itoe, Robert P. St.Onge, Celia Quevedo, Andrea Ruecker, Paul Henri Amvam Zollo, Francisco-Javier Gamo, Nathan Lee, Alvine Ngoutane Mfopa, Paul Horrocks, Ikuo Igarashi, Nil Gural, Todd R. Golub, Gordana Panic, Jeremy N. Burrows, Phat Voong Vinh, Annette Kaiser, Fabrice Fekam Boyom, Pietro Alano, Anupam Pradhan, Sandra Duffy, Raj N. Misra, Vidya Prasanna Kumar, Aintzane Alday, Timothy N. C. Wells, María Isabel Loza, Sébastien Kicka, William J. Sullivan, Gregory M. Goldgof, Yo Suzuki, Yolanda Corbett, Sally-Ann Poulsen, Vida Ahyong, George Papadatos, Sujeevi Nawaratna, Rafaela Salgado Ferreira, Takaaki Horii, Imran Ullah, Nathalie Narraidoo, Natalie G. Robinett, Simon V. Avery, Grazia Camarda, Iset Medina Vera, Michael T. Ferdig, Fengwu Li, David Plouffe, Joseph L. DeRisi, Jasmeet Samra, Andreas Spitzmüller, Liqiong Liu, Christopher A. Rice, Thierry Soldati, Serge Maximilian Stamm, Suzanne Gokool, Beatrice L. Colon, Shimaa Abd El-Salam El-Sayed, Mark Baker, Kenneth O. Udenze, Na Le Dang, Katrin Ingram-Sieber, Dennis A. Smith, Rays H. Y. Jiang, Marian P. Brennan, Ani Galstian, Paul Willis, Dennis E. Kyle, Ainhoa Alzualde, Sarah Prats, Sheena McGowan, Vicky M. Avery, Jennifer Keiser, John P. Moore, Dalu Mancama, Gregory J. Crowther, Noemi Cowan, Maria B. Cassera, Valentin Trofimov, David Thomas, David J. Sullivan, Diana Ortiz, Nada Abla, S. Joshua Swamidass, Benjamin Blasco, Hoan Vu, Francesco Silvestrini, Anthony J. Chubb, Pamela M. White, Scott M. Landfear, Isabelle Florent, John H. Adams, Ronald J. Quinn, Andrew F. Wilks, Sandra March, Leonardo Lucantoni, Stephen Baker, Tana Bowling, Joachim Müller, Arantza Muriana, Lauren E. Boucher, Ajit Jadhav, Sukjun Lee, Elizabeth A. Winzeler, Choukri Ben Mamoun, Ulrich Schlecht, Daisy D. Colón-López, Marjorie Schmitt, Myles H. Akabas, Isabelle S Lucet, Stephen N. Hewitt, Naoaki Yokoyama, Carl Nathan, Bakela Nare, Cindy Vallières, Lotfi Bounaadja, Kayode K. Ojo, Wesley Wu, Ken Chih-Chien Cheng, Kathryn F. Tonissen, Michael J. Delves, Brian M. Suzuki, Aristea Lubar, Quentin D. Bickle, Stephan Meister, Silvia Parapini, Manuel Llinás, Ngoc Minh Pham, Seunghyun Moon, R. Kiplin Guy, Donatella Taramelli, Lawrence Ayong, Sarah D'Alessandro, Jürgen Bosch, David Little

Publikováno v: PLoS Pathogens, Vol 12, Iss 7, p e1005763 (2016)
Van Voorhis, Wesley C; Adams, John H; Adelfio, Roberto; Ahyong, Vida; Akabas, Myles H; Alano, Pietro; Alday, Aintzane; Alemán Resto, Yesmalie; Alsibaee, Aishah; Alzualde, Ainhoa; Andrews, Katherine T; Avery, Simon V; Avery, Vicky M; Ayong, Lawrence; Baker, Mark; Baker, Stephen; Ben Mamoun, Choukri; Bhatia, Sangeeta; Bickle, Quentin; Bounaadja, Lotfi; ... (2016). Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond. PLoS pathogens, 12(7), e1005763. Public Library of Science 10.1371/journal.ppat.1005763
PLoS Pathogens
PLoS Pathogens, Public Library of Science, 2016, 12 (7), pp.e1005763. ⟨10.1371/journal.ppat.1005763⟩
PLoS pathogens
Recercat. Dipósit de la Recerca de Catalunya
instname
PLOS
PLOS PATHOGENS
e1005763
PLOS Pathogens
PLOS Pathogens, Vol. 12, No 7 (2016) P. e1005763

A major cause of the paucity of new starting points for drug discovery is the lack of interaction between academia and industry. Much of the global resource in biology is present in universities, whereas the focus of medicinal chemistry is still larg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed1aa7e406ea2a739a6d5c26550fa093
https://doaj.org/article/18b88a00726d44659ebd19c6f7c84d1b