Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Stephen M. McIlfatrick"'
Autor:
Andrew M. Lew, Nella Fisicaro, Wayne J. Hawthorne, Peter J. Cowan, Mark B. Nottle, Jamie L. Brady, Evelyn J Salvaris, Ivan Vassiliev, Stephen M. McIlfatrick, Philip J. O'Connell
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
Scientific Reports
Scientific Reports
Xenotransplantation from pigs has been advocated as a solution to the perennial shortage of donated human organs and tissues. CRISPR/Cas9 has facilitated the silencing of genes in donor pigs that contribute to xenograft rejection. However, the genera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de8968a79880f2ed22568c7bcc3defdd
https://doaj.org/article/13fe765c6d834f9facf6f7bbc3879a6c
https://doaj.org/article/13fe765c6d834f9facf6f7bbc3879a6c
Publikováno v:
Theriogenology. 86:1008-1013
The present study was undertaken to examine the effect of feed restriction on ovulation rate and in vivo blastocyst development in gilts and sows. In the first experiment, gilts were feed restricted (1 vs. 2.5 times maintenance requirement) during th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b5a58c6a02bc832a6fe7621bfd97bf4
https://doi.org/10.1016/j.theriogenology.2016.03.029
https://doi.org/10.1016/j.theriogenology.2016.03.029
Autor:
Stephen M. McIlfatrick, Sharon J. Harrison, Peter J. Cowan, P. J. O'connell, Evelyn J Salvaris, Anthony J F D'Apice, Luke F.S. Beebe, Sandra Pommey, Nella Fisicaro, Mark B. Nottle, R.J. Ashman
Publikováno v:
Xenotransplantation. 14:339-344
We report here our experience regarding the production of double or homozygous Gal knockout (Gal KO) pigs by breeding and somatic cell nuclear transfer (SCNT). Large White x Landrace female heterozygous Gal KO founders produced using SCNT were mated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db8ad5fd33a1b7ea110fabf6c6fdf13a
https://doi.org/10.1111/j.1399-3089.2007.00417.x
https://doi.org/10.1111/j.1399-3089.2007.00417.x
Publikováno v:
Cloning & Transgenesis.
We have previously demonstrated that the use of porcine mesenchymal stem cells (MSCs) isolated from the bone marrow can increase the proportion of somatic cell nuclear transfer (SCNT) embryos that develop to the blastocyst stage compared with adult f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::995f86f1970251b0990ed858b7593f2a
https://doi.org/10.4172/2168-9849.1000136
https://doi.org/10.4172/2168-9849.1000136
Autor:
Paul Tolstoshev, Anthony J F D'Apice, Christopher G. Grupen, Peter J. Cowan, Ian Lyons, Allan J. Robins, Christopher Giannakis, A.C. Boquest, Stephen M. McIlfatrick, Renate Faast, Angelo Guildolin, Lesley Crocker, R.J. Ashman, James Wengle, Philip J. O'Connell, Mark B. Nottle, Sharon J. Harrison
Publikováno v:
Scopus-Elsevier
We have reported relatively efficient methods for somatic cell nuclear transfer and for knocking out the alpha(1,3)-galactosyltransferase (alpha1,3-GT) gene in porcine fetal fibroblasts using a nonisogenic promoterless construct approach. Here we rep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::015db991c60b52ca3337c7a1c19f1e95
https://doi.org/10.1089/clo.2004.6.327
https://doi.org/10.1089/clo.2004.6.327
Autor:
Mark B. Nottle, Stephen M. McIlfatrick, R.J. Ashman, A.C. Boquest, Sharon J. Harrison, Christopher G. Grupen, Anthony J. F. D'apice
Publikováno v:
Biology of Reproduction. 66:1283-1287
Somatic cell nuclear transfer was used to produce live piglets from cultured fetal fibroblast cells. This was achieved by exposing donor cell nuclei to oocyte cytoplasm for approximately 3 h before activation by chemical means. Initially, an experime
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::867e5ffaf36acee9d7a32774d433235d
https://doi.org/10.1095/biolreprod66.5.1283
https://doi.org/10.1095/biolreprod66.5.1283
Autor:
Mark B. Nottle, Christopher G. Grupen, Zhong-Tao Du, Stephen M. McIlfatrick, Paul J. Verma, Ian Lyons, Renate Faast, R.J. Ashman, Lesley Crocker
Publikováno v:
Molecular Reproduction and Development. 57:262-269
The in vitro development of porcine nuclear transfer embryos constructed using primary cultures from day 25 fetal fibroblasts which were either rapidly dividing (cycling) or had their cell-cycle synchronized in G0/G1 using serum starvation (serum-sta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::899e812fa4b931d294c905152bf9c45d
https://doi.org/10.1002/1098-2795(200011)57:3<262::aid-mrd8>3.0.co
https://doi.org/10.1002/1098-2795(200011)57:3<262::aid-mrd8>3.0.co
Publikováno v:
Cloning & Transgenesis.
Work in our laboratory for more than two decades has focussed on the production of genetically modified pigs for xeno transplantion research. More recent work has focussed on the isolation of porcine embryonic stem cells to facilitate this as well as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ec37619175b3d45d0979f5f5f5df73d5
https://doi.org/10.4172/2168-9849.1000107
https://doi.org/10.4172/2168-9849.1000107
Autor:
Mark B. Nottle, Svetlana Vassilieva, Stephen M. McIlfatrick, Kam P. Truong, Ivan Vassiliev, Sharon J. Harrison, Luke F.S. Beebe
Publikováno v:
Cellular reprogramming. 13(3)
We report here the establishment and characterization of putative porcine embryonic stem cell (ESC) lines derived from somatic cell nuclear transfer embryos (NT-ESCs). These cells had a similar morphology to that described previously by us for ESCs d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3273bf58a7c0d3d6ecb3b1a6ef6af5ec
https://pubmed.ncbi.nlm.nih.gov/21548828
https://pubmed.ncbi.nlm.nih.gov/21548828
Publikováno v:
Theriogenology. 75(8)
The objective was to develop a simple successful porcine cryopreservation protocol that prevented contact between embryos and liquid nitrogen, avoiding potential contamination risks. In vivo-derived blastocysts were collected surgically from donor pi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a36905e04a8bcc874bfe1deaab1264b5
https://pubmed.ncbi.nlm.nih.gov/21220168
https://pubmed.ncbi.nlm.nih.gov/21220168