Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Stephen M. G. Duff"'
Publikováno v:
PLoS ONE, Vol 16, Iss 12 (2021)
Glutamate decarboxylase (GAD; EC 4.1.1.15) catalyzes the irreversible decarboxylation of glutamate to produce γ-aminobutyric acid (GABA); a ubiquitous non-protein amino acid involved in the regulation of several aspects of plant metabolism and physi
Externí odkaz:
https://doaj.org/article/5ee1527526c6449e9c7815bbf4ee0cbb
Autor:
Stephen M. G. Duff, Keith Kretzmer
Publikováno v:
Journal of Plant Biochemistry and Biotechnology.
In an attempt to increase source capacity, transgenic corn was generated by expressing a truncated maize sucrose phosphate synthase (ZmSPSΔ482) under two leaf mesophyll cellspecific promoters (CAB and PPDK). The endogenous and truncated SPS proteins
Publikováno v:
International Journal of Plant Physiology and Biochemistry. 10:28-75
Cytokinins (CK) are key determinants of seed size and their level can impact crop yield. The biological activities and chemistry of cytokinins are well defined but less is known about their molecular mode of action during maize kernel development, es
Autor:
Loïc Lepiniec, Pierre Gadal, Gautam Sarath, Stephen M. G. Duff, Shirley A. Condon, Valérie Pacquit, Jean Vidal, Raymond Chollet, Carlos S. Andreo
Publikováno v:
European Journal of Biochemistry. 228:92-95
Steady-state kinetic analyses were performed on the non-phosphorylated, in vitro phosphorylated and phosphorylation-site mutant (Ser8Asp) forms of purified recombinant sorghum C4 phosphoewo/pyruvate (P-pyruvate) carboxylase (EC 4.1.1.31) containing a
Autor:
K. S. Gellatly, Daniel D. Lefebvre, William C. Plaxton, Greg B.G. Moorhead, Stephen M. G. Duff
Publikováno v:
Plant Physiology. 106:223-232
The major acid phosphatase (APase) from potato (Solanum tuberosom L. cv Chiefton) tubers has been purified 2289-fold to near homogeneity and a final O-phospho-L-tyrosine (P-Tyr) hydrolyzing specific activity of 1917 [mu]mol Pi produced min-1 mg-1 of
Publikováno v:
Physiologia Plantarum. 90:791-800
Hydrolysis of phosphate esters is a critical process in the energy metabolism and metabolic regulation of plant ceils. This review summarizes the characteristics and putative roles of piant acid phosphatase lAPasel. Although immunologicaliy closely r
Autor:
Pierre Gadal, Jean Vidal, Claude Crétin, Loïc Lepiniec, G. Sarath, C.S. Andreo, Stephen M. G. Duff, S.A. Condon, R. Chollet
Publikováno v:
Archives of Biochemistry and Biophysics. 306:272-276
A recombinant, site-directed mutant form of sorghum phosphoenolpyruvate carboxylase (PEPC), in which the phosphorylatable serine residue (Ser-8) was changed to cysteine (S8C), was chemically modified by iodoacetic acid and iodoacetamide for the purpo
Publikováno v:
Journal of Biological Chemistry. 267:21901-21905
PP(i)-dependent phosphofructokinase (PFP) activity, measured in the forward direction, increased approximately 19-fold when suspension cell cultures of black mustard (Brassica nigra) were subjected to 18 days of P(i) deprivation. Fructose 2,6-bisphos
Publikováno v:
Archives of Biochemistry and Biophysics. 286:226-232
An acid phosphatase from Brassica nigra (black mustard) leaf petiole cell-suspension cultures has been purified 1633-fold to a final specific activity of 1225 (μmol orthophosphate produced/min)/mg protein and near homogeneity. The native protein was
Autor:
Cheryl A. Fife, William C. Plaxton, Daniel D. Lefebvre, Stephen M. G. Duff, Colin Julien-Inalsingh
Publikováno v:
Plant Physiology. 93:504-511
Suspension cells of Brassica nigra responded to Pi deprivation by increasing their potential for Pi influx and by raising the active levels of intracellular, cell surface, and secreted acid phosphatases. These responses, however, were temporally dist