Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Stephen L. Sazinsky"'
Autor:
Raffi Tonikian, Yingnan Zhang, Stephen L Sazinsky, Bridget Currell, Jung-Hua Yeh, Boris Reva, Heike A Held, Brent A Appleton, Marie Evangelista, Yan Wu, Xiaofeng Xin, Andrew C Chan, Somasekar Seshagiri, Laurence A Lasky, Chris Sander, Charles Boone, Gary D Bader, Sachdev S Sidhu
Publikováno v:
PLoS Biology, Vol 6, Iss 9, p e239 (2008)
PDZ domains are protein-protein interaction modules that recognize specific C-terminal sequences to assemble protein complexes in multicellular organisms. By scanning billions of random peptides, we accurately map binding specificity for approximatel
Externí odkaz:
https://doaj.org/article/4ed87eb30eb5451a85620aede5da754a
Autor:
Tiffany F. Chen, Stephen L. Sazinsky, David J. DiLillo, Julie Bird, Huawei Qiu, Kevin K. Li, John R. Engen, K. Dane Wittrup, Jeffrey V. Ravetch, Damian Houde, George T. Cheng
Publikováno v:
PMC
© 2017 Elsevier Ltd The binding of human IgG1 to human Fc gamma receptors (hFcγRs) is highly sensitive to the presence of a single N-linked glycosylation site at asparagine 297 of the Fc, with deglycosylation resulting in a complete loss of hFcγR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e211e3a765a22c223837394b25362cf
https://pubmed.ncbi.nlm.nih.gov/28694069
https://pubmed.ncbi.nlm.nih.gov/28694069
Autor:
Elizabeth A. Villar, Stephen L. Sazinsky, Brandon S. Zerbe, Mary S. Golden, R. Georgiadis, Sandor Vajda, Adrian Whitty, Shaun M. Cote, Marianna K. Sayeg, Dmitri Beglov, Dima Kozakov
Publikováno v:
Journal of the American Chemical Society. 135:6242-6256
We report a comprehensive analysis of binding energy hot spots at the protein-protein interaction (PPI) interface between nuclear factor kappa B (NF-κB) essential modulator (NEMO) and IκB kinase subunit β (IKKβ), an interaction that is critical f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::baf5423cdbf7b7e690f687a9b2193095
https://doi.org/10.1021/ja400914z
https://doi.org/10.1021/ja400914z
Autor:
K. Dane Wittrup, Bruce Tidor, Rene G. Ott, Jeffrey V. Ravetch, Stephen L. Sazinsky, Nathaniel W. Silver
Publikováno v:
Proceedings of the National Academy of Sciences. 105:20167-20172
Immunoglobulin G plays a vital role in adaptive immunity and antibody-based therapy through engagement of its Fc region by the Fc gamma receptors (Fc gammaRs) on immune cells. In addition to specific protein-protein contacts, N-linked glycosylation o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfe9d6c4b8f81cd98b560893a7faeb66
https://doi.org/10.1073/pnas.0809257105
https://doi.org/10.1073/pnas.0809257105
Autor:
Ping Wu, Sachdev S. Sidhu, Stephen L. Sazinsky, Nicholas J. Skelton, Brent A. Appleton, Christian Wiesmann, Borlan Pan, Yingnan Zhang, Steven T. Runyon
Publikováno v:
Protein Science. 16:2454-2471
High-temperature requirement A (HtrA) and its homologs contain a serine protease domain followed by one or two PDZ domains. Bacterial HtrA proteins and the mitochondrial protein HtrA2/Omi maintain cell function by acting as both molecular chaperones
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d065c258b8dbf4c51d33993ad0b9b68a
https://doi.org/10.1110/ps.073049407
https://doi.org/10.1110/ps.073049407
Autor:
Eric S. Day, James D Wright, Mariya Atanasova, Christopher Donnelly, Sandra Schlee, Paul Carmillo, Thomas V. Riera, Stephen L. Sazinsky, Adrian Whitty
Publikováno v:
The FASEB Journal. 24
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a43c3e2307b06b812a8bde152b972a51
https://doi.org/10.1096/fasebj.24.1_supplement.200.3
https://doi.org/10.1096/fasebj.24.1_supplement.200.3
Autor:
Shirley Hui, Stephen L. Sazinsky, Sachdev S. Sidhu, Bridget Currell, Gary D. Bader, Somasekar Seshagiri, Andreas Ernst, Moyez Dharsee
Publikováno v:
Science Signaling. 2
Multicellular organisms rely on complex, fine-tuned protein networks to respond to environmental changes. We used in vitro evolution to explore the role of domain mutation and expansion in the evolution of network complexity. Using random mutagenesis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea54be3648e07fa5bbea5702cd73d655
https://doi.org/10.1126/scisignal.2000416
https://doi.org/10.1126/scisignal.2000416
Publikováno v:
Biotechnology and bioengineering. 103(6)
Because of its eukaryotic nature, simple fermentation requirements, and pliable genetics, there have been many attempts at improving recombinant protein production in S. cerevisiae. These strategies typically involve altering the expression of a nati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a99b736a8c0b3415125f317319ad8e59
https://pubmed.ncbi.nlm.nih.gov/19459139
https://pubmed.ncbi.nlm.nih.gov/19459139
Autor:
Boris Reva, Andrew C. Chan, Laurence A. Lasky, Raffi Tonikian, Xiaofeng Xin, Charles Boone, Somasekar Seshagiri, Brent A. Appleton, Yan-Yan Wu, Bridget Currell, Chris Sander, Jung-Hua Yeh, Sachdev S. Sidhu, Yingnan Zhang, Gary D. Bader, Stephen L. Sazinsky, Heike A. Held, Marie Evangelista
Publikováno v:
PLoS Biology, Vol 6, Iss 9, p e239 (2008)
PLoS Biology
PLoS Biology
PDZ domains are protein–protein interaction modules that recognize specific C-terminal sequences to assemble protein complexes in multicellular organisms. By scanning billions of random peptides, we accurately map binding specificity for approximat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c618625ddd30b6e40babbca8b2d1510
http://europepmc.org/articles/PMC2553845?pdf=render
http://europepmc.org/articles/PMC2553845?pdf=render
Autor:
Ginger Chao, Stephen L. Sazinsky, Wai L. Lau, Shaun M. Lippow, K. Dane Wittrup, Benjamin J. Hackel
Publikováno v:
Nature protocols. 1(2)
This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display. Single-chain antibody fragments (scFvs) are first isolated from an existing nonimmune human libr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a17a8dc934605e97cde61ff5f5d2f748
https://pubmed.ncbi.nlm.nih.gov/17406305
https://pubmed.ncbi.nlm.nih.gov/17406305