Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Stephen E. Badger"'
Autor:
Ian J. Bruce, Peter R. Levison, Dieter Schimkat, Prit Hathi, Stephen E. Badger, Jon W. Dennis, Martin J. Davies
Publikováno v:
Journal of Chromatography A. 816:107-111
The performance of Magarose, an agarose-based bead containing a paramagnetic component has been evaluated. The anion exchanger DEAE-Magarose is effective at binding DNA from a crude cell lysate. The plasmid pBluescript was isolated from 1.5 ml Escher
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc97a81a0e4feb85055dc2d9397643a6
https://doi.org/10.1016/s0021-9673(98)00064-8
https://doi.org/10.1016/s0021-9673(98)00064-8
Autor:
Stephen E. Badger, Anne Brandt-Nielsen, Peter R. Levison, Carol Mumford, Navin D. Pathirana, Michael Streater
Publikováno v:
Journal of Chromatography A. 760:151-158
The physical and functional performance of 70 different anion- and cation-exchange media based on cellulose, agarose, dextran and polymeric/composite materials has been evaluated. Physical tests such as swelling, flow performance and column packing d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cbd218980977a366e6dd5f3507d55ca4
https://doi.org/10.1016/s0021-9673(96)00814-x
https://doi.org/10.1016/s0021-9673(96)00814-x
Autor:
Navin D. Pathirana, S.J. Wheeler, David W. Toome, Jones Russell Mark, Peter R. Levison, Michael Streater, Stephen E. Badger
Publikováno v:
Journal of Chromatography A. 702:59-68
The effectiveness of a clean-in-place procedure involving treatment with 0.5 M NaOH for 16 h at room temperature has been examined following process-scale chromatography of 1.85 kg hen egg-white proteins on the DEAE-cellulose, Whatman Express-Ion Exc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d750f04f60c2b876e7dc44c29041e7e6
https://doi.org/10.1016/0021-9673(94)00991-h
https://doi.org/10.1016/0021-9673(94)00991-h
Publikováno v:
Journal of Chromatography A. 658:419-428
The scale-up of the separation of hen egg-white proteins was investigated using Whatman DEAE-cellulose/D856, a fast-flowing microgranular anion-exchange cellulose. Under suitable conditions the maximum binding capacity for ovalbumin was determined to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ad2413c61b3231d793572ecba8a5f413
https://doi.org/10.1016/0021-9673(94)80032-4
https://doi.org/10.1016/0021-9673(94)80032-4
Autor:
Edward T. Butts, Mark L. Koscielny, Stephen E. Badger, Linda Lane, David W. Toome, Peter R. Levison
Publikováno v:
Journal of Chromatography A. 590:49-58
The scale-up of the separation of hen egg-white proteins has been investigated using Whatman DE92 anion-exchange cellulose. Having developed suitable chromatographic conditions, a maximum binding capacity of 100 mg protein/ml packed DE92 was determin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e673706914fca214055cf54d04d684b8
https://doi.org/10.1016/0021-9673(92)87005-s
https://doi.org/10.1016/0021-9673(92)87005-s
Autor:
Fred Mann, Nicola Dickson, Prit Hathi, Peter R. Levison, Anne K Hopkins, Geoff Purdom, Stephen E. Badger
Publikováno v:
Journal of chromatography. A. 890(1)
A new technique in downstream processing, suspended bed chromatography has been developed. This hybrid technique exploiting the benefits of batch adsorption and the process advantages of an enclosed column system can be carried out using established
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99dee6a3a6340225ebab5017560af251
https://pubmed.ncbi.nlm.nih.gov/10976793
https://pubmed.ncbi.nlm.nih.gov/10976793
Autor:
Ian J. Bruce, Peter R. Levison, Stephen E. Badger, Prit Hathi, Volker Grimm, Martin J. Davies
Publikováno v:
Journal of chromatography. A. 827(2)
The performance of different anion-exchange media have been compared for the isolation of plasmid DNA and genomic DNA from bacterial cells and human whole blood. Whatman DEAE-Magarose, based on an agarose bead containing a paramagnetic component, has
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::199e0ca86d0c871f5b9cd6eeaa30fbc7
https://pubmed.ncbi.nlm.nih.gov/9914660
https://pubmed.ncbi.nlm.nih.gov/9914660
Publikováno v:
Separations for Biotechnology 2 ISBN: 9789401068390
Whatman DE52 (tertiary amine substituted anion-exchange cellulose) has been shown to exhibit fast kinetics of adsorption and high capacity for proteins of molecular mass up to 670 kDa. These features lend themselves to process-scale use and we have i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::40b094e774cadbe8abb9ad5f3c9b57cf
https://doi.org/10.1007/978-94-009-0783-6_41
https://doi.org/10.1007/978-94-009-0783-6_41
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