Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Stephen D. Minchin"'
Publikováno v:
Methods. 47:6-12
In bacteria, the expression of transcription units is controlled by regulatory regions, that contain one or more promoters and binding sites for regulatory proteins that activate or repress expression in response to different signals. In this chapter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22778380edce8d27c8173fe3a8dc4409
https://doi.org/10.1016/j.ymeth.2008.10.012
https://doi.org/10.1016/j.ymeth.2008.10.012
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1628:22-29
Connexin32 (Cx32) is the predominant gap junction protein expressed in adult rat hepatocytes. This study investigated transcriptional regulation of the rat Cx32 gene in MH 1 C 1 rat hepatoma cells using transient expression assays in conjunction with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c33e4ab1d3819e78d44e2bc1559543b
https://doi.org/10.1016/s0167-4781(03)00111-8
https://doi.org/10.1016/s0167-4781(03)00111-8
Publikováno v:
FEBS Letters. 544:199-205
Previous work has shown that the base sequence of the DNA segment immediately upstream of the -10 hexamer at bacterial promoters (the extended -10 element) can make a significant contribution to promoter strength. Guided by recently published structu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce097c9fbd8a45ad3d577c3758d7d9c6
https://doi.org/10.1016/s0014-5793(03)00500-3
https://doi.org/10.1016/s0014-5793(03)00500-3
Publikováno v:
Nucleic Acids Research. 27:2051-2056
We have studied the role of the C-terminal domain of the alpha subunit (alphaCTD) of Escherichia coli RNA polymerase during transcription initiation at promoters lacking an UP-element. The temperature requirement for open complex formation was used a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c68697ad41f438f8145b9e9a8f2274
https://doi.org/10.1093/nar/27.9.2051
https://doi.org/10.1093/nar/27.9.2051
Autor:
Stephen J. W. Busby, Claude F. Meares, Nobuyuki Fujita, Jeffrey T. Owens, Jonathan A. Bown, Akira Ishihama, Stephen D. Minchin
Publikováno v:
Journal of Biological Chemistry. 274:2263-2270
A cysteine-tethered DNA cleavage agent has been used to locate the position of region 2.5 of ς70 in transcriptionally competent complexes between Escherichia coli RNA polymerase and promoters. In this study we have engineered ς70 to introduce a uni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a0bf4aac7d5828703c7bb925a400b5e
https://doi.org/10.1074/jbc.274.4.2263
https://doi.org/10.1074/jbc.274.4.2263
Publikováno v:
The EMBO Journal. 16:4034-4040
At some bacterial promoters, a 5'-TG-3' sequence element, located one base upstream of the -10 hexamer element, provides an essential motif necessary for transcription initiation. We have identified a mutant of the Escherichia coli RNA polymerase sig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bdc7f4db79c82d828a08c602b62ce4a
https://doi.org/10.1093/emboj/16.13.4034
https://doi.org/10.1093/emboj/16.13.4034
Publikováno v:
Annual review of microbiology. 66
Bacteria use a variety of mechanisms to direct RNA polymerase to specific promoters in order to activate transcription in response to growth signals or environmental cues. Activation can be due to factors that interact at specific promoters, thereby
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73303004e9e3c729aadd203bd2318fb7
https://pubmed.ncbi.nlm.nih.gov/22726217
https://pubmed.ncbi.nlm.nih.gov/22726217
Publikováno v:
European Journal of Biochemistry. 225:1195-1201
We have overexpressed human cardiac troponin-I in Escherichia coli. Initially, protein expression was not detected in the bacterial cell extracts. Systematic deletion of the N-terminal region of the protein generated a series of truncated mutants whi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89a00402c8d5ffee409583d424730970
https://doi.org/10.1111/j.1432-1033.1994.1195b.x
https://doi.org/10.1111/j.1432-1033.1994.1195b.x
Autor:
Helen D. Burns, Stephen D. Minchin
Publikováno v:
Nucleic Acids Research. 22:3840-3845
We have studied the role of extended protein DNA contacts and DNA topology on the ability of Escherichia coli RNA polymerase to form open complexes at several related promoters. The -35 region of several Escherichia coli promoters do not have homolog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7d057d41f784bf56ed4c73bfe025cf1
https://doi.org/10.1093/nar/22.19.3840
https://doi.org/10.1093/nar/22.19.3840
Autor:
Steve Busby, Stephen D. Minchin
Publikováno v:
Biochemical Journal. 289:771-775
Methylation-interference assays have been used to identify guanine residues that make important contacts with RNA polymerase during open-complex formation at two related Escherichia coli promoters. Methylation of lower-strand G-31 at a gal consensus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0a6099fc43044724776d5e9581bcd95
https://doi.org/10.1042/bj2890771
https://doi.org/10.1042/bj2890771