Small molecule inhibitors of RAS-effector protein interactions derived using an intracellular antibody fragment

Autor: Camilo E. Quevedo, Abimael Cruz-Migoni, Nicolas Bery, Ami Miller, Tomoyuki Tanaka, Donna Petch, Carole J. R. Bataille, Lydia Y. W. Lee, Phillip S. Fallon, Hanna Tulmin, Matthias T. Ehebauer, Narcis Fernandez-Fuentes, Angela J. Russell, Stephen B. Carr, Simon E. V. Phillips, Terence H. Rabbitts

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)

Intracellular antibodies can inhibit disease-relevant protein interactions, but inefficient cellular uptake limits their utility. Using a RAS-targeting intracellular antibody as a screening tool, the authors here identify small molecules that inhibit

Externí odkaz: https://doaj.org/article/67c80be1d0a14b3aa0feecf6b7e3b832

The adaptability of the ion binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF

Autor: Ryan M. Lithgo, Marko Hanževački, Gemma Harris, Jos J. A. G. Kamps, Ellie Holden, Justin LP Benesch, Christof M. Jäger, Anna K. Croft, Jon L. Hobman, Allen M. Orville, Andrew Quigley, Stephen B. Carr, David J. Scott

The periplasmic chaperone SilF has been identified as part of an Ag(I) detoxification system in Gram negative bacteria. Sil proteins also bind Cu(I), but with reported weaker affinity, therefore leading to the designation of a specific detoxification

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c40d543e6ffbf50775a16df9ea6ac890
https://doi.org/10.1101/2023.03.31.534560

BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions

Autor: Nicolas Bery, Abimael Cruz-Migoni, Carole JR Bataille, Camilo E Quevedo, Hanna Tulmin, Ami Miller, Angela Russell, Simon EV Phillips, Stephen B Carr, Terence H Rabbitts

Publikováno v: eLife, Vol 7 (2018)

The RAS family of proteins is amongst the most highly mutated in human cancers and has so far eluded drug therapy. Currently, much effort is being made to discover mutant RAS inhibitors and in vitro screening for RAS-binding drugs must be followed by

Externí odkaz: https://doaj.org/article/6d8a1e8ab8724fc883df0eb1fdb7cab0

Aerobic Photocatalytic H2 Production by a [NiFe] Hydrogenase Engineered to Place a Silver Nanocluster in the Electron Relay

Autor: Giorgio Morello, Stephen B. Carr, Fraser A. Armstrong, Liyun Zhang

Publikováno v: Journal of the American Chemical Society. 142:12699-12707

Hydrogenase-1 (Hyd-1) from E. coli poses a conundrum regarding the properties of electrocatalytic reversibility and associated bidirectionality now established for many redox enzymes. Its excellent H2-oxidizing activity begins only once a substantial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0a599fc1dd414deca8c6047a74e93a7e
https://doi.org/10.1021/jacs.0c04302

The crystalline state as a dynamic system: IR microspectroscopy under electrochemical control for a [NiFe] hydrogenase

Autor: Rhiannon M. Evans, Amelia Brasnett, Adam J. Healy, Kylie A. Vincent, Jack S. Rowbotham, Gianfelice Cinque, Ricardo Hidalgo, Philip A. Ash, Fraser A. Armstrong, Stephen B. Carr, Mark D. Frogley, Sophie E. T. Kendall-Price, Simone Morra

Publikováno v: Chemical Science
Ash, P A, Kendall-Price, S E T, Evans, R M, Carr, S B, Brasnett, A R, Morra, S, Rowbotham, J, Hidalgo, R, Cinque, G, Frogley, M D, Armstrong, F A & Vincent, K 2021, ' The crystalline state as a dynamic system: IR microspectroscopy under electrochemical control for a [NiFe] hydrogenase ', Chemical Science, vol. 12, no. 39, pp. 12959-12970 . https://doi.org/10.1039/d1sc01734a

Controlled formation of catalytically-relevant states within crystals of complex metalloenzymes represents a significant challenge to structure–function studies. Here we show how electrochemical control over single crystals of [NiFe] hydrogenase 1

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c127f5e49347962dfbe4f0160410c0b1
https://pubmed.ncbi.nlm.nih.gov/34745526