Zobrazeno 1 - 10
of 185
pro vyhledávání: '"Stephen A. Bustin"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-18 (2024)
Abstract Extracellular vesicles have emerged as important mediators of cell-to-cell communication in the pathophysiology of fibrotic diseases. One such disease is Peyronie’s disease (PD), a fibrotic disorder of the penis caused by uncontrolled tran
Externí odkaz:
https://doaj.org/article/2b2ffd1e0fee454c91c49f05b0d84e33
Autor:
Keertan Dheda, Jim F. Huggett, Stephen A. Bustin, Margaret A. Johnson, Graham Rook, Alimuddin Zumla
Publikováno v:
BioTechniques, Vol 37, Iss 1, Pp 112-119 (2004)
Analysis of RNA expression using techniques like real-time PCR has traditionally used reference or housekeeping genes to control for error between samples. This practice is being questioned as it becomes increasingly clear that some housekeeping gene
Externí odkaz:
https://doaj.org/article/3d7fc50f42c94d24b43b4b5cc247c4b6
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 1303, p 1303 (2022)
International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1303
International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1303
Reverse transcription of RNA coupled to amplification of the resulting cDNA by the polymerase chain reaction (RT-PCR) is one of the principal molecular technologies in use today, with applications across all areas of science and medicine. In its real
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cfb6489a71ef52c9cf71cd3c0abbefa
https://arro.anglia.ac.uk/id/eprint/707330/1/Bustin_et_al_2022.pdf
https://arro.anglia.ac.uk/id/eprint/707330/1/Bustin_et_al_2022.pdf
Publikováno v:
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 8702, p 8702 (2021)
Volume 22
Issue 16
International Journal of Molecular Sciences, Vol 22, Iss 8702, p 8702 (2021)
Volume 22
Issue 16
The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is an established tool for the diagnosis of RNA pathogens. Its potential for automation has caused it to be used as a presence/absence diagnostic tool even when RNA quantifica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47097b63338735bea21f26c7f39bc5f9
https://doi.org/10.20944/preprints202107.0604.v2
https://doi.org/10.20944/preprints202107.0604.v2
Autor:
Stephen A. Bustin, Stuart L. Simpson, Kyle Bibby, Aaron Bivins, Devrim Kaya, Orin C. Shanks, Warish Ahmed
Publikováno v:
Water Research
Due to the coronavirus disease 2019 (COVID-19) pandemic, wastewater surveillance has become an important tool for monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within communities. In particular, reverse transcr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97e60732d7a99ed91fe20c08735e930a
http://europepmc.org/articles/PMC8341816
http://europepmc.org/articles/PMC8341816
Autor:
Stuart L. Simpson, Devrim Kaya, Warish Ahmed, Aaron Bivins, Stephen A. Bustin, Kyle Bibby, Orin C. Shanks
The coronavirus disease 2019 (COVID-19) pandemic has led to wastewater surveillance becoming an important tool for monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within communities. As a result, molecular method
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be2acdb48dac333b622fb8cb5dd2bdc1
Publikováno v:
PLoS ONE, Vol 9, Iss 2, p e88266 (2014)
The quantitative real time polymerase chain reaction (qPCR) has become a key molecular enabling technology with an immense range of research, clinical, forensic as well as diagnostic applications. Its relatively moderate instrumentation and reagent r
Externí odkaz:
https://doaj.org/article/0fb60285f18242439d2c5610619110e6
Publikováno v:
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific reports, 10:22214
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific reports, 10:22214
Accurate, reliable and rapid detection of SARS-CoV-2 is essential not only for correct diagnosis of individual COVID-19 disease but also for the development of a rational strategy aimed at lifting confinement restrictions and preparing for possible r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d079619709737aca3b8e02b871bd28fc
Autor:
Jo Vandesompele, Jacob Moran-Gilad, Michael W. Pfaffl, Timothy D. McHugh, Heinz Zeichhardt, Gregory L. Shipley, Marc L. Salit, Jim F. Huggett, Vladimir Benes, Jeremy A. Garson, Peter M. Vallone, Carl T. Wittwer, Mikael Kubista, Kathryn A. Harris, Martin Kammel, Stephen A. Bustin, Tania Nolan
Publikováno v:
Clinical Chemistry
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f01237b206a02bf592a1b9a7cc9e6cc
Autor:
Stephen A. Bustin, M. Mateus, Trinity J. Bivalacqua, Asif Muneer, David Ralph, Michael J. Carpenter, Selim Cellek, Simon J. Stafford, Marcus M. Ilg
Background Myofibroblast transformation is a key step in the pathogenesis of Peyronie's disease (PD). Phosphodiesterase type 5 inhibitors (PDE5is) and selective estrogen receptor modulators (SERMs) can prevent the formation of fibrosis in in vitro an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f26cddc82d7dd55f62c92c1bce7a1610
https://arro.anglia.ac.uk/id/eprint/705818/1/Ilg_2020.pdf
https://arro.anglia.ac.uk/id/eprint/705818/1/Ilg_2020.pdf