Zobrazeno 1 - 10
of 161
pro vyhledávání: '"Stephen, Buratowski"'
Autor:
Hyun Jin Bae, Marion Dubarry, Jongcheol Jeon, Luis M. Soares, Catherine Dargemont, Jaehoon Kim, Vincent Geli, Stephen Buratowski
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Methylation of histone H3 lysine 4 (H3K4) by Set1/COMPASS occurs co-transcriptionally, and is important for gene regulation. Here the authors provide evidence that the Set1 N-terminal region and the COMPASS subunit Swd2 interact with RNA polymerase I
Externí odkaz:
https://doaj.org/article/f45ecf358a5a4b8a8f4052500b0aec19
Autor:
Victoria A. Church, Sigal Pressman, Mamiko Isaji, Mary Truscott, Nihal Terzi Cizmecioglu, Stephen Buratowski, Maxim V. Frolov, Richard W. Carthew
Publikováno v:
Cell Reports, Vol 20, Iss 13, Pp 3123-3134 (2017)
The cellular abundance of mature microRNAs (miRNAs) is dictated by the efficiency of nuclear processing of primary miRNA transcripts (pri-miRNAs) into pre-miRNA intermediates. The Microprocessor complex of Drosha and DGCR8 carries this out, but it ha
Externí odkaz:
https://doaj.org/article/18f0c1c2733240598accbdfcb27f21a8
Autor:
Ji Hyun Kim, Bo Bae Lee, Young Mi Oh, Chenchen Zhu, Lars M. Steinmetz, Yookyeong Lee, Wan Kyu Kim, Sung Bae Lee, Stephen Buratowski, TaeSoo Kim
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
H3K36 methylation by Set2 targets Rpd3S histone deacetylase to transcribed mRNA genes, repressing internal cryptic promoters and modulating elongation. Here, the authors provide evidence that the Set2-Rpd3S pathway also regulates dynamic expression o
Externí odkaz:
https://doaj.org/article/dbf3891b60f2429294504f03df887f75
Autor:
Dorine Jeanne Mariëtte du Mee, Maxim Ivanov, Joseph Paul Parker, Stephen Buratowski, Sebastian Marquardt
Publikováno v:
eLife, Vol 7 (2018)
Most DNA in the genomes of higher organisms does not code for proteins. RNA Polymerase II (Pol II) transcribes non-coding DNA into long non-coding RNAs (lncRNAs), but biological roles of lncRNA are unclear. We find that mutations in the yeast lncRNA
Externí odkaz:
https://doaj.org/article/6d63143c8c6041d286974897936fc05c
Publikováno v:
Cell Reports, Vol 6, Iss 6, Pp 961-972 (2014)
Methylation of histone H3 lysine 4 by the Set1 subunit of COMPASS correlates with active transcription. Here, we show that Set1 levels are regulated by protein degradation in response to multiple signals. Set1 levels are greatly reduced when COMPASS
Externí odkaz:
https://doaj.org/article/d3dab47551814e0d87694ab626fb8ad0
Publikováno v:
G3 (Bethesda, Md.). 12(10)
Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stabili
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c89ff21a9229d4d359436edbe0e1daf7
https://pubmed.ncbi.nlm.nih.gov/35944214
https://pubmed.ncbi.nlm.nih.gov/35944214
Autor:
Kun Ping Lu, Hyuk-Soo Seo, Yujin Chun, Christopher M. Browne, Theresa D. Manz, Xiao Zhen Zhou, Zainab M. Doctor, Xiaolan Lian, Zoe C. Yeoh, Shin Kibe, Scott B. Ficarro, Nir London, Benika J. Pinch, Nicholas E. Vangos, Behnam Nabet, Stephen Buratowski, Dina Daitchman, Shingo Kozono, Daniel Zaidman, Nathanael S. Gray, Jarrod A. Marto, Mikaela L. Mohardt, Li Tan, Sirano Dhe-Paganon, Ezekiel A. Geffken
Publikováno v:
Nature chemical biology
Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (Pin1) is commonly overexpressed in human cancers, including pancreatic ductal adenocarcinoma (PDAC). While Pin1 is dispensable for viability in mice, it is required for activated Ras to induce t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6565cbd6de180dcf92ba1d74b5e545e0
https://doi.org/10.1038/s41589-020-0550-9
https://doi.org/10.1038/s41589-020-0550-9
Autor:
Yoo Jin Joo, Stephen Buratowski
Publikováno v:
Mol Cell Biol
In our previously published studies, RNA polymerase II transcription initiation complexes were assembled from yeast nuclear extracts onto immobilized transcription templates and analyzed by quantitative mass spectrometry. In addition to the expected
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02c4a0a4b3eb60268603be98d1d7b246
https://pubmed.ncbi.nlm.nih.gov/34694912
https://pubmed.ncbi.nlm.nih.gov/34694912
SUMMARYRNA polymerase II (Pol II) transcription reconstituted from purified factors suggests pre-initiation complexes (PICs) can assemble by sequential incorporation of factors at the TATA box. However, these basal transcription reactions are general
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63bbddd0ce85c01d10553d52d002963a
Publikováno v:
Methods. :96-104
The RNA polymerase II (RNApII) transcription cycle consists of multiple steps involving dozens of protein factors. Here we describe a useful approach to study the dynamics of initiation and early elongation, comprising an in vitro transcription syste
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc21e97664470eb440627f2ed973450a
https://doi.org/10.1016/j.ymeth.2019.03.001
https://doi.org/10.1016/j.ymeth.2019.03.001