Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris

Autor: Stephanie P. Cartwright, Roslyn M. Bill, Bui Tien Sy, Hieu Tran-Van, Hung Minh Nguyen

Publikováno v: BMC Research Notes, Vol 10, Iss 1, Pp 1-6 (2017)

Abstract Objective Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepa

Externí odkaz: https://doaj.org/article/f925542113ea4bdf9371d505014137c0

L-carnosine affects the growth of Saccharomyces cerevisiae in a metabolism-dependent manner.

Autor: Stephanie P Cartwright, Roslyn M Bill, Alan R Hipkiss

Publikováno v: PLoS ONE, Vol 7, Iss 9, p e45006 (2012)

The dipeptide L-carnosine (β-alanyl-L-histidine) has been described as enigmatic: it inhibits growth of cancer cells but delays senescence in cultured human fibroblasts and extends the lifespan of male fruit flies. In an attempt to understand these

Externí odkaz: https://doaj.org/article/7915666cf77d43acb2ec0fad85aca619

Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics

Autor: Justin A. MacDonald, Stephanie P. Cartwright, Rena Far, Jay Kim, Annegret Ulke-Lemée, Roslyn M. Bill

Publikováno v: Methods in Molecular Biology ISBN: 9781493990238

New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ffebb4b0755ff09a7d1b2430ffec8c7f
https://doi.org/10.1007/978-1-4939-9024-5_8

Constitutively-stressed yeast strains are high-yielding for recombinant Fps1:Implications for the translational regulation of an aquaporin

Autor: Richard A. J. Darby, Stephane R. Gross, Roslyn M. Bill, Debasmita Sarkar, Mark P. Ashe, Stephanie P. Cartwright, Nicklas Bonander

Publikováno v: Cartwright, S P, Darby, R A J, Sarkar, D, Bonander, N, Gross, S R, Ashe, M P & Bill, R M 2017, ' Constitutively-stressed yeast strains are high-yielding for recombinant Fps1 : Implications for the translational regulation of an aquaporin ', Microbial Cell Factories, vol. 16, no. 1, 41 . https://doi.org/10.1186/s12934-017-0656-2
Microbial Cell Factories

Background We previously selected four strains of Saccharomyces cerevisiae for their ability to produce the aquaporin Fps1 in sufficient yield for further study. Yields from the yeast strains spt3Δ, srb5Δ, gcn5Δ and yTHCBMS1 (supplemented with 0.5

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f3177c23937555f49c52850cf7ecf37
https://doi.org/10.1186/s12934-017-0656-2

The synthesis of recombinant membrane proteins in yeast for structural studies

Autor: Michelle Clare, Martin D.B. Wilks, Anjana Patel, Sarah J Routledge, Zharain Bawa, Lina Mikaliunaite, Floren Low, Roslyn M. Bill, David Hardy, Stephanie P. Cartwright, Alice Rothnie

Historically, recombinant membrane protein production has been a major challenge meaning that many fewer membrane protein structures have been published than those of soluble proteins. However, there has been a recent, almost exponential increase in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01f371cd1b650a42b5272049279d5979
https://publications.aston.ac.uk/id/eprint/27300/1/Synthesis_of_recombinant_membrane_proteins_in_yeast.pdf