Molecular characterization of the CXCR4 / CXCR7 axis in germ cell tumors and its targetability using nanobody-drug-conjugates

Autor: Gamal A. Wakileh, Philipp Bierholz, Mara Kotthoff, Margaretha A. Skowron, Felix Bremmer, Alexa Stephan, Stephanie M. Anbuhl, Raimond Heukers, Martine J. Smit, Philipp Ströbel, Daniel Nettersheim

Publikováno v: Experimental Hematology & Oncology, Vol 12, Iss 1, Pp 1-5 (2023)

Abstract Being stimulated by the chemokine CXCL12, the CXCR4 / CXCR7 cascade is involved in tumor proliferation, migration, and metastasis. The interaction between CXCL12, secreted by cells from the microenvironment, and its receptors is complex and

Externí odkaz: https://doaj.org/article/7c03766aefb5452d89e9a05381e22674

NanoB2 to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET

Autor: Jelle van den Bor, Nick D. Bergkamp, Stephanie M. Anbuhl, Françoise Dekker, Dehan Comez, Claudia V. Perez Almeria, Reggie Bosma, Carl W. White, Laura E. Kilpatrick, Stephen J. Hill, Marco Siderius, Martine J. Smit, Raimond Heukers

Publikováno v: Cell Rep Methods
Cell Reports Methods, 3(3):100422, 1-20. Cell Press
van den Bor, J, Bergkamp, N D, Anbuhl, S M, Dekker, F, Comez, D, Perez Almeria, C V, Bosma, R, White, C W, Kilpatrick, L E, Hill, S J, Siderius, M, Smit, M J & Heukers, R 2023, ' NanoB 2 to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET ', Cell Reports Methods, vol. 3, no. 3, 100422, pp. 1-20 . https://doi.org/10.1016/j.crmeth.2023.100422

The therapeutic potential of ligands targeting disease-associated membrane proteins is predicted by ligand-receptor binding constants, which can be determined using NanoLuciferase (NanoLuc)-based bioluminescence resonance energy transfer (NanoBRET) m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::187bb179073083438689811500689316
https://hdl.handle.net/1871.1/fd6e8c26-f260-45e0-b457-6f76b80d3e3a