Enzyme-less nanopore detection of post-translational modifications within long polypeptides

Autor: Pablo Martin-Baniandres, Wei-Hsuan Lan, Stephanie Board, Mercedes Romero-Ruiz, Sergi Garcia-Manyes, Yujia Qing, Hagan Bayley

Means to sequence DNA and RNA quickly and cheaply have revolutionized biology and medicine. The ability to analyse cellular proteins and their millions of variants would be an advance of comparable importance, but requires a fresh technical approach.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c48b5b58d70198b249deb927081eb411
https://ora.ox.ac.uk/objects/uuid:fecef13a-219c-4658-b5e3-5227cd687282

Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape

Autor: Rafael Tapia-Rojo, Marc Mora, Stephanie Board, Jane Walker, Rajaa Boujemaa-Paterski, Ohad Medalia, Sergi Garcia-Manyes

Publikováno v: Tapia-Rojo, R, Mora, M, Board, S, Walker, J, Boujemaa-Paterski, R, Medalia, O & Garcia-Manyes, S 2022, ' Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape ', Nature Physics, pp. 52–60 . https://doi.org/10.1038/s41567-022-01808-4

Statistical mechanics can describe the major conformational ensembles determining the equilibrium free-energy landscape of a folding protein. The challenge is to capture the full repertoire of low-occurrence conformations separated by high kinetic ba

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4c77d225dc74b729ca606f6a4bd4bea

The mechanical stability of proteins regulates their translocation rate into the cell nucleus

Autor: Elvira Infante, Yongjian Wang, Catherine M. Shanahan, Stephanie Board, Andrew Stannard, Samuel A. Gulaidi Breen, Ainhoa Lezamiz, Palma Rico-Lastres, Fani Panagaki, Vinoth Sundar Rajan, Elena Rostkova, Pere Roca-Cusachs, Sergi Garcia-Manyes, Amy E. M. Beedle

Publikováno v: Infante, E, Stannard, A, Board, S J, Rico-Lastres, P, Rostkova, E, Beedle, A E M, Lezamiz, A, Wang, Y J, Gulaidi Breen, S, Panagaki, F, Sundar Rajan, V, Shanahan, C, Roca-Cusachs, P & Garcia-Manyes, S 2019, ' The mechanical stability of proteins regulates their translocation rate into the cell nucleus ', Nature Physics, vol. 15, no. 9, pp. 973-981 . https://doi.org/10.1038/s41567-019-0551-3
Publons
Nature Physics

A cell’s ability to react to mechanical stimuli is known to be affected by the transport of transcription factors, the proteins responsible for regulating transcription of DNA into RNA, across the membrane enveloping its nucleus. Yet the molecular

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44c515c138bed1ed5dac5723562d2c70
https://kclpure.kcl.ac.uk/ws/files/113466324/The_mechanical_stability_of_INFANTE_Published_1_July_2019_GREEEN_AAM.pdf