In vivo CRISPR screening identifies geranylgeranyl diphosphate as a pancreatic cancer tumor growth dependency

Autor: Casie S. Kubota, Stephanie L. Myers, Toni T. Seppälä, Richard A. Burkhart, Peter J. Espenshade

Publikováno v: Molecular Metabolism, Vol 85, Iss , Pp 101964- (2024)

Objective: Cancer cells must maintain lipid supplies for their proliferation and do so by upregulating lipogenic gene programs. The sterol regulatory element-binding proteins (SREBPs) act as modulators of lipid homeostasis by acting as transcriptiona

Externí odkaz: https://doaj.org/article/d8ae974546924ca99dcf0bbe44ca3440

Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism

Autor: Alfica Sehgal, Debaditya Mukhopadhyay, Michael J. Wolfgang, Meredith R. McGuire, Kai Kammers, Rita T. Brookheart, Namandjé N. Bumpus, Eric P. Mosher, Peter J. Espenshade, Ebru S. Selen, Stephanie L. Myers

Publikováno v: The Journal of Biological Chemistry

Progesterone receptor membrane component 1 (PGRMC1) is a heme-binding protein implicated in a wide range of cellular functions. We previously showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and supports their activity. Recen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c095f0e4cbf517a7bff30d6370b0944
https://doi.org/10.1016/j.jbc.2021.101316

Requirement of Sterol Regulatory Element‐Binding Protein Pathway in Pancreatic Ductal Adenocarcinoma

Autor: Meredith R. McGuire, Peter J. Espenshade, Zeshaan A. Rasheed, William Matsui, Wei Shao, Stephanie L. Myers, Ted Ewachiw

Publikováno v: The FASEB Journal. 33

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::dbff4adab39045c286ad7d2a0a7da294
https://doi.org/10.1096/fasebj.2019.33.1_supplement.652.14

High-throughput screening and hit validation of extracellular-related kinase 5 (ERK5) inhibitors

Autor: Timothy J. Blackburn, Roger J. Griffin, Tim Hammonds, Betty Cottyn, Ian R. Hardcastle, Sandrine Vidot, Lauren Molyneux, Bernard T. Golding, Celine Cano, William Clegg, Ruth H. Bawn, Louise Bisset, Hing Y. Leung, David R. Newell, Ai-Ching Wong, Stephanie M. Myers, Laurent Rigoreau, Ross W. Harrington

Publikováno v: ACS Combinatorial Science
ACS Combinatorial Science, ACS, 2016, 18, pp.444-455. ⟨10.1021/acscombsci.5b00155⟩
ACS Combinatorial Science (18), 444-455. (2016)

International audience; The extracellular-related kinase 5 (ERK5) is a promising target for cancer therapy. A high-throughput screen was developed for ERK5, based on the IMAP FP progressive binding system, and used to identify hits from a library of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::002ffb5033573e80911c966b43ada6d9
https://eprints.gla.ac.uk/139945/1/139945.pdf

SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load

Autor: Melissa A. McAlexander, Adam L. Moyer, Diego A. Espinoza, Erin L. Buchanan, Stephanie L. Myers, Kenneth W. Witwer

Publikováno v: Scientific Reports

Restriction of HIV-1 in myeloid-lineage cells is attributed in part to the nucleotidase activity of the SAM-domain and HD-domain containing protein (SAMHD1), which depletes free nucleotides, blocking reverse transcription. In the same cells, the Vpx

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ab34126323a4d7ca0a69e5f329dd818