Výsledky vyhledávání - "Stephan Lambotte"

Orientational Distribution of α-Helices in the Colicin B and E1 Channel Domains: A One and Two Dimensional 15N Solid-State NMR Investigation in Uniaxially Aligned Phospholipid Bilayers

Autor: Burkhard Bechinger, Stephan Lambotte, Pieter Jasperse

Publikováno v: Biochemistry. 37:16-22

Thermolytic fragments of the channel-forming bacterial toxins colicin B and colicin E1 were uniformly labeled with the 15N isotope and reconstituted into uniaxially oriented membranes. These well-aligned samples were investigated by proton-decoupled

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d99dc39cf83726fbdbd9e044feae62c2
https://doi.org/10.1021/bi9724671

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The Human Gene of a Protein That Modifies Na+-d-Glucose Co-Transport

Autor: Rolf K. H. Kinne, Maike Veyhl, Alison I. Morrison-Shetlar, Michael Schmid, Hermann Koepsell, Stephan Lambotte, Michael Köhler

Publikováno v: DNA and Cell Biology. 15:769-777

Recently, a cDNA (pRS1) was cloned from pig kidney cortex that encodes a membrane-associated protein involved in Na(+)-coupled sugar transport. pRS1 alters sugar transport by SGLT1 from rabbit intestine or by SMIT from dog kidney which is homologous

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d066b5c1e90b6313d7381bf31604f7ea
https://doi.org/10.1089/dna.1996.15.769

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Deciphering Membrane Insertion of the Diphtheria Toxin T Domain by Specular Neutron Reflectometry and Solid-State NMR Spectroscopy

Autor: Alexandre, Chenal, Lydia, Prongidi-Fix, Aurélie, Perier, Christopher, Aisenbrey, Grégory, Vernier, Stephan, Lambotte, Michael, Haertlein, Marie-Thérèse, Dauvergne, Giovanna, Fragneto, Burkhard, Bechinger, Daniel, Gillet, Vincent, Forge, Michel, Ferrand

Publikováno v: Journal of Molecular Biology
Journal of Molecular Biology, 2009, 391 (5), pp.872-883. ⟨10.1016/j.jmb.2009.06.061⟩
Journal of Molecular Biology, Elsevier, 2009, 391 (5), pp.872-883. ⟨10.1016/j.jmb.2009.06.061⟩

International audience; Insertion and translocation of soluble proteins into and across biological membranes are involved in many physiological and pathological processes, but remain poorly understood. Here, we describe the pH-dependent membrane inse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83e5f674445465d2a9bddb813cdd9a2a
https://hal.science/hal-01151673

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Specific isotope labeling of colicin E1 and B channel domains for membrane topological analysis by oriented solid-state NMR spectroscopy

Autor: Monica Cusan, Julia Georgescu, Pieter Jasperse, Burkhard Bechinger, Christopher Aisenbrey, Stephan Lambotte, Ulrike Harzer

Publikováno v: Chembiochem : a European journal of chemical biology. 9(6)

An approach is presented to selectively label the methionines of the colicin E1 and B channel domains, each about 200 residues in size, and use them for oriented solid-state NMR investigations. By combining site-directed mutagenesis, bacterial overex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5473a378641f25797327f5e489a0f8ec
https://pubmed.ncbi.nlm.nih.gov/18338351

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Calorimetric investigations of the structural stability and interactions of colicin B domains in aqueous solution and in the presence of phospholipid bilayers

Autor: Burkhard Bechinger, Stephan Lambotte, Alicia Ortega

Publikováno v: The Journal of biological chemistry. 276(17)

The effects of pH and temperature on the stability of interdomain interactions of colicin B have been studied by differential-scanning calorimetry, circular dichroism, and fluorescence spectroscopy. The calorimetric properties were compared with thos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abb0fe7ef3ac938c0361e87be4d5eb79
https://pubmed.ncbi.nlm.nih.gov/11278359

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Corrigendum to 'Deciphering Membrane Insertion of the Diphtheria Toxin T Domain by Specular Neutron Reflectometry and Solid-State NMR Spectroscopy' [J. Mol. Biol. (2009) 391, 872–883]

Autor: Alexandre Chenal, Christopher Aisenbrey, Lydia Prongidi-Fix, Michel Ferrand, Grégory Vernier, Vincent Forge, Daniel Gillet, Michael Haertlein, Aurélie Perier, Marie-Thérèse Dauvergne, Stephan Lambotte, Giovanna Fragneto, Burkhard Bechinger

Publikováno v: Journal of Molecular Biology. 394:587

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e205ede2849b4af30f78c310ed129cc7
https://doi.org/10.1016/j.jmb.2009.10.005

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Orientational Distribution of α-Helices in the Colicin B and El Channel Domains: A One and Two Dimensional 15N Solid-State NMR Investigation in Uniaxially Aligned Phospholipid Bilayers

Autor: Stephan Lambotte, Pieter Jasperse, Burkhard Bechinger

Publikováno v: Biochemistry. 37:18128-18128

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1ff6ba6c2fc88af6adc484f2527b4904
https://doi.org/10.1021/bi985050j

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