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of 20
pro vyhledávání: '"Stephan Hirschi"'
Autor:
Stephan Hirschi, Thomas Lemmin, Nooraldeen Ayoub, David Kalbermatter, Daniele Pellegata, Zöhre Ucurum, Jürg Gertsch, Dimitrios Fotiadis
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)
Abstract Microbial ion-pumping rhodopsins (MRs) are extensively studied retinal-binding membrane proteins. However, their biogenesis, including oligomerisation and retinal incorporation, remains poorly understood. The bacterial green-light absorbing
Externí odkaz:
https://doaj.org/article/a108a1ee9b9d4375998fcce708237c39
Autor:
Abhishek Jamwal, Cristina F Constantin, Stephan Hirschi, Sebastian Henrich, Wolfgang Bildl, Bernd Fakler, Simon J Draper, Uwe Schulte, Matthew K Higgins
Publikováno v:
eLife, Vol 12 (2023)
Basigin is an essential host receptor for invasion of Plasmodium falciparum into human erythrocytes, interacting with parasite surface protein PfRH5. PfRH5 is a leading blood-stage malaria vaccine candidate and a target of growth-inhibitory antibodie
Externí odkaz:
https://doaj.org/article/b9aa1a147c4e481b9a59a36c9d0bcded
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
The cryo-EM structure of pentameric green-light absorbing proteorhodopsin together with molecular dynamics simulations and functional studies provides insights into the proton translocation pathway and oligomerization, and a protonation-dependent mec
Externí odkaz:
https://doaj.org/article/be15953b5e8e49b3be8f4c74426e812d
Publikováno v:
Journal of Structural Biology: X, Vol 4, Iss , Pp 100024- (2020)
The green-light absorbing proteorhodopsin (GPR) is the prototype of bacterial light-driven proton pumps. It has been the focus of continuous research since its discovery 20 years ago and has sparked the development and application of various biophysi
Externí odkaz:
https://doaj.org/article/e14727547c1940b9b8b38396b4e0fe83
Autor:
Mirko Stauffer, Stephan Hirschi, Zöhre Ucurum, Daniel Harder, Ramona Schlesinger, Dimitrios Fotiadis
Publikováno v:
Methods and Protocols, Vol 3, Iss 3, p 51 (2020)
The light-driven proton pump bacteriorhodopsin (BR) from the extreme halophilic archaeon Halobacterium salinarum is a retinal-binding protein, which forms highly ordered and thermally stable 2D crystals in native membranes (termed purple membranes).
Externí odkaz:
https://doaj.org/article/d7f1aa4c173a49d19ae0fb20b0a831ca
Autor:
Stephan Hirschi, Mirko Stauffer, Daniel Harder, Daniel J. Müller, Wolfgang Meier, Dimitrios Fotiadis
Publikováno v:
CHIMIA, Vol 70, Iss 6 (2016)
Synthetic biology approaches range from the introduction of unique features into organisms to the assembly of isolated biomacromolecules or synthetic building blocks into artificial biological systems with biomimetic or completely novel functionaliti
Externí odkaz:
https://doaj.org/article/22d8b58a89fe465f813905aa596228d3
Publikováno v:
Chemical Reviews. 122:16294-16328
The bottom-up assembly of biological and chemical components opens exciting opportunities to engineer artificial vesicular systems for applications with previously unmet requirements. The modular combination of scaffolds and functional building block
Structural and functional characterization of proteins as well as the design of targeted drugs heavily rely on recombinant protein expression and purification. The polyhistidine-tag (His-tag) is among the most prominent examples of affinity tags used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c162d3c0356477423fe53c7a6a190772
Autor:
Stephan, Hirschi, Dimitrios, Fotiadis
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2127
A protocol is described for the isolation of recombinant polyhistidine-tagged membrane proteins from overexpressing Escherichia coli cells. The gene encoding a target membrane protein is cloned into an expression plasmid and then introduced into E. c
Publikováno v:
Journal of Structural Biology: X, Vol 4, Iss, Pp 100024-(2020)
Journal of Structural Biology: X
Journal of Structural Biology: X
Graphical abstract
Highlights • Affinity tag-free isolation of proteorhodopsin (PR) by ion exchange and size exclusion chromatography. • Biochemical and computational analysis indicate a single, pentameric PR population. • Highly pure and
Highlights • Affinity tag-free isolation of proteorhodopsin (PR) by ion exchange and size exclusion chromatography. • Biochemical and computational analysis indicate a single, pentameric PR population. • Highly pure and