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Autor:
Albert Spielmann, Jochen Kuhla, Thomas Dr. Hottiger, Jutta Heim, M.B. Garn, Peter Fürst, Gabriele Pohlig, Stephan Haemmerli
Publikováno v:
Yeast (Chichester, England). 11(1)
We have constructed 2-micron-based yeast expression vectors containing a copy of the metallothionein (CUP1) gene of Saccharomyces cerevisiae as a semi-dominant, selectable marker. When used for the expression of the thrombin inhibitor hirudin, origin