Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Stella M. Lai"'
Publikováno v:
Biomolecules, Vol 6, Iss 2, p 22 (2016)
RNase P, a ribozyme-based ribonucleoprotein (RNP) complex that catalyzes tRNA 5′-maturation, is ubiquitous in all domains of life, but the evolution of its protein components (RNase P proteins, RPPs) is not well understood. Archaeal RPPs may provid
Externí odkaz:
https://doaj.org/article/726d4ca17c094572bb5a156ab265babc
Autor:
Ryan D. Kibler, Sangmin Lee, Madison A. Kennedy, Basile I. M. Wicky, Stella M. Lai, Marius M. Kostelic, Xinting Li, Cameron M. Chow, Lauren Carter, Vicki H. Wysocki, Barry L. Stoddard, David Baker
Pseudosymmetric hetero-oligomers with three or more unique subunits with overall structural (but not sequence) symmetry play key roles in biology, and systematic approaches for generating such proteinsde novowould provide new routes to controlling ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2628e2e7ede05f8a26f2b79c0c760e93
https://doi.org/10.1101/2023.04.07.535760
https://doi.org/10.1101/2023.04.07.535760
Autor:
Stella M. Lai, Vicki H. Wysocki, Ila A Marathe, Walter J. Zahurancik, Michael G. Poirier, Venkat Gopalan
Publikováno v:
Nucleic Acids Research
The ribonucleoprotein (RNP) form of archaeal RNase P comprises one catalytic RNA and five protein cofactors. To catalyze Mg2+-dependent cleavage of the 5′ leader from pre-tRNAs, the catalytic (C) and specificity (S) domains of the RNase P RNA (RPR)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d8f6ee716051189ffbcca751e329eeb
https://doi.org/10.1093/nar/gkab655
https://doi.org/10.1093/nar/gkab655
Autor:
Lien B. Lai, Stella M. Lai, Eric S. Szymanski, Mridu Kapur, Edric K. Choi, Hashim M. Al-Hashimi, Susan L. Ackerman, Venkat Gopalan
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, vol 119, iss 10
Significance Understanding and treating neurological disorders are global priorities. Some of these diseases are engendered by mutations that cause defects in the cellular synthesis of transfer RNAs (tRNAs), which function as adapter molecules that t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b77e97535501754e746664c371a233
https://pubmed.ncbi.nlm.nih.gov/35238631
https://pubmed.ncbi.nlm.nih.gov/35238631
Publikováno v:
Biochemistry
Protein overexpression and purification are critical for in vitro structure-function characterization studies. However, some proteins are difficult to express in heterologous systems due to host-related (e.g., codon usage, translation rate) and/or pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc12558a01103d4a5a8de89e8a0e9058
https://pubmed.ncbi.nlm.nih.gov/34100589
https://pubmed.ncbi.nlm.nih.gov/34100589
Autor:
Stella M. Lai, F. Curtis Hewitt, Kathleen Q. Schulte, Andrew J. Reed, Alan R. Smith, Danielle S. LeSassier, Maryam Baniasad, Liwen Zhang, Michael A. Freitas, Myles W. Gardner, August E. Woerner, Vicki H. Wysocki, Katharina L. Weber
Publikováno v:
Journal of proteomics. 249
We present an efficient protein extraction and in-solution enzymatic digestion protocol optimized for mass spectrometry-based proteomics studies of human skin samples. Human skin cells are a proteinaceous matrix that can enable forensic identificatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4dd8ee1ea5283dc899df982d94b4e89
https://pubmed.ncbi.nlm.nih.gov/34481086
https://pubmed.ncbi.nlm.nih.gov/34481086
Protein overexpression and purification are critical for in vitro structure-function characterization studies. However, some proteins are difficult to express robustly in heterologous systems due to host-related (e.g., codon usage, translation rate)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76e653dc7bbde3820564a73306031466
https://doi.org/10.26434/chemrxiv.14035691
https://doi.org/10.26434/chemrxiv.14035691
Autor:
Vicki H. Wysocki, Vaishnavi Sidharthan, Venkat Gopalan, Jamison D Law, Andrew Norris, Stella M. Lai, Pankajavalli Thirugnanasambantham
Publikováno v:
Methods Enzymol
Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems ISBN: 9780323901468
Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems ISBN: 9780323901468
Purification of recombinant proteins typically entails overexpression in heterologous systems and subsequent chromatography-based isolation. While denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis is routinely used to screen a vari
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89840f07138739d8d9f6b1b832696908
https://europepmc.org/articles/PMC9055603/
https://europepmc.org/articles/PMC9055603/
Autor:
Venkat Gopalan, Stella M. Lai, Walter J. Zahurancik, Dalton T. Snyder, Andrew Norris, Vicki H. Wysocki
Publikováno v:
Methods Enzymol
Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems ISBN: 9780323901468
Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems ISBN: 9780323901468
The ubiquitous ribonucleoprotein (RNP) form of RNase P catalyzes the Mg(2+)-dependent cleavage of the 5′ leader of precursor-transfer RNAs. The rate and fidelity of the single catalytic RNA subunit in the RNase P RNP is significantly enhanced by as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dece129fca167518c84d5082360c11f
https://europepmc.org/articles/PMC9099236/
https://europepmc.org/articles/PMC9099236/
Autor:
Venkat Gopalan, Stella M. Lai
Publikováno v:
Methods in Molecular Biology ISBN: 9781071607152
Owing to an oversight, the words "Reverse" and "Cleaved" in Subheading 2.3.3 of Chapter 9 were spelt incorrectly in the book.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fab4f04d55d39186c471d11c87e9f49b
https://doi.org/10.1007/978-1-0716-0716-9_17
https://doi.org/10.1007/978-1-0716-0716-9_17