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A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation.

Autor: Strawn IK; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA., Steiner PJ; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA., Newton MS; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA., Baumer ZT; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA., Whitehead TA; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2023 Oct; Vol. 120 (10), pp. 3057-3066. Date of Electronic Publication: 2023 Jun 27.

Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds.

Autor: Beltrán J; Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA.; Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, USA., Steiner PJ; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA., Bedewitz M; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA., Wei S; Department of Biochemistry, University of California, Riverside, Riverside, CA, USA., Peterson FC; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA., Li Z; Department of Chemistry, University of California, Riverside, Riverside, CA, USA., Hughes BE; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA., Hartley Z; Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA.; Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, USA., Robertson NR; Department of Bioengineering, University of California, Riverside, Riverside, USA., Medina-Cucurella AV; Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI, USA., Baumer ZT; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA., Leonard AC; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA., Park SY; Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA., Volkman BF; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA., Nusinow DA; Donald Danforth Plant Science Center, St. Louis, MO, USA., Zhong W; Department of Chemistry, University of California, Riverside, Riverside, CA, USA., Wheeldon I; Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, USA. wheeldon@ucr.edu.; Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA, USA. wheeldon@ucr.edu., Cutler SR; Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA. cutler@ucr.edu.; Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, USA. cutler@ucr.edu.; Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, USA. cutler@ucr.edu., Whitehead TA; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, USA. timothy.whitehead@colorado.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2022 Dec; Vol. 40 (12), pp. 1855-1861. Date of Electronic Publication: 2022 Jun 20.

Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning.

Autor: Haas CM; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO 80303, USA., Francino-Urdaniz IM; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO 80303, USA., Steiner PJ; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO 80303, USA., Whitehead TA; Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO 80303, USA.

Publikováno v: STAR protocols [STAR Protoc] 2021 Dec 17; Vol. 2 (4), pp. 100869. Date of Electronic Publication: 2021 Sep 22.

Paired heavy- and light-chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responses.

Autor: Banach BB; Bioengineering Graduate Program, University of Kansas, Lawrence, KS 66045, USA., Cerutti G; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Fahad AS; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Shen CH; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA., Oliveira De Souza M; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Katsamba PS; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Tsybovsky Y; Electron Microscopy Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA., Wang P; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Nair MS; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Huang Y; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Francino-Urdániz IM; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Steiner PJ; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Gutiérrez-González M; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Liu L; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., López Acevedo SN; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Nazzari AF; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA., Wolfe JR; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Luo Y; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Olia AS; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA., Teng IT; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA., Yu J; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Zhou T; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA., Reddem ER; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Bimela J; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Pan X; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Madan B; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Laflin AD; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Nimrania R; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA., Yuen KY; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China; Department of Microbiology, Queen Mart Hospital, Hong Kong Special Administrative Region, China; Department of Clinical Microbiology and Infection Control, University of Hong Kong-Shenzhen Hospital, Shenzhen, China., Whitehead TA; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA., Ho DD; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Kwong PD; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA., Shapiro L; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA. Electronic address: lss8@columbia.edu., DeKosky BJ; Bioengineering Graduate Program, University of Kansas, Lawrence, KS 66045, USA; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA; Department of Chemical Engineering, University of Kansas, Lawrence, KS 66045, USA. Electronic address: dekosky@ku.edu.

Publikováno v: Cell reports [Cell Rep] 2021 Oct 05; Vol. 37 (1), pp. 109771. Date of Electronic Publication: 2021 Sep 28.