Nonhydrolysable Analogues of (p)ppGpp and (p)ppApp Alarmone Nucleotides as Novel Molecular Tools

Autor: Hiraku Takada, Duy Dinh Do Pham, Abel Garcia-Pino, Magdalena Petrová, Hedvig Tamman, Dominik Rejman, Radek Pohl, Hanna Ainelo, Julien Caballero-Montes, Viktor Mojr, Steffi Jimmy, Vasili Hauryliuk, Katleen Van Nerom, Mohammad Roghanian

Publikováno v: A C S Chemical Biology

While alarmone nucleotides guanosine-3′,5′-bisdiphosphate (ppGpp) and guanosine-5′-triphosphate-3′-diphosphate (pppGpp) are archetypical bacterial second messengers, their adenosine analogues ppApp (adenosine-3′,5′-bisdiphosphate) and ppp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84d0b5e32d8c65a347400f85a2022ce0
https://doi.org/10.1021/acschembio.1c00398

A hyperpromiscuous antitoxin protein domain for the neutralization of diverse toxin domains

Autor: Tatsuaki, Kurata, Chayan Kumar, Saha, Jessica A, Buttress, Toomas, Mets, Tetiana, Brodiazhenko, Kathryn J, Turnbull, Ololade F, Awoyomi, Sofia Raquel Alves, Oliveira, Steffi, Jimmy, Karin, Ernits, Maxence, Delannoy, Karina, Persson, Tanel, Tenson, Henrik, Strahl, Vasili, Hauryliuk, Gemma C, Atkinson

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 119(6)

Toxin-antitoxin (TA) gene pairs are ubiquitous in microbial chromosomal genomes and plasmids as well as temperate bacteriophages. They act as regulatory switches, with the toxin limiting the growth of bacteria and archaea by compromising diverse esse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::44c8344a78a0e48975dd3dfc8a88427d
https://pubmed.ncbi.nlm.nih.gov/35121656

A widespread toxin−antitoxin system exploiting growth control via alarmone signaling

Autor: Albinas Cepauskas, Hiraku Takada, Alan Koh, Henrik Strahl, Constantine Stavropoulos, Steffi Jimmy, Vasili Hauryliuk, Tanel Tenson, Abel Garcia-Pino, Gemma C. Atkinson, Dominik Rejman, Sofia Raquel Alves Oliveira, Chayan Kumar Saha, Tatsuaki Kurata

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 117 (19
Proceedings of the National Academy of Sciences of the United States of America

Under stressful conditions, bacterial RelA-SpoT Homolog (RSH) enzymes synthesize the alarmone (p)ppGpp, a nucleotide second messenger. (p)ppGpp rewires bacterial transcription and metabolism to cope with stress, and, at high concentrations, inhibits

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f0b2ccc0aad5e7d59f85809fcc77baf
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/307329

Ribosome Association Primes the Stringent Factor Rel for Recruitment of Deacylated tRNA to the Ribosomal A-Site

Autor: Steffi Jimmy, Vasili Hauryliuk, Pavel Kudrin, Rikinori Murayama, Fabio Trebini, Mohammad Roghanian, Julien Caballero-Montes, Katleen Van Nerom, Hiraku Takada, Genki Akanuma, Abel Garcia-Pino

Publikováno v: SSRN Electronic Journal.

In Bacillus subtilis, amino acid starvation induces synthesis of the alarmone (p)ppGpp by the bifunctional RelA/SpoT Homolog factor Rel, capable of both synthesising and hydrolysing (p)ppGpp. Rel monitors the aminoacylation status of the ribosomal A-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cfb7c4fb4e428f20d81969f2cfb51abb
https://doi.org/10.2139/ssrn.3600424

Molecular mutagenesis of ppGpp: turning a RelA activator into an inhibitor

Autor: Dominik Rejman, Tanel Tenson, Jelena Beljantseva, Victoria Shingler, Vallo Varik, Steffi Jimmy, Vasili Hauryliuk, Radek Pohl, Yuzuru Tozawa, Marcel Ehn, Pavel Kudrin

Publikováno v: Scientific Reports

The alarmone nucleotide (p)ppGpp is a key regulator of bacterial metabolism, growth, stress tolerance and virulence, making (p)ppGpp-mediated signaling a promising target for development of antibacterials. Although ppGpp itself is an activator of the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c0b8b6b6fcd7093d9e6c62772a51405
https://doi.org/10.1038/srep41839