Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Steffen M. Sparvath"'
Autor:
Mette D. E. Jepsen, Steffen M. Sparvath, Thorbjørn B. Nielsen, Ane H. Langvad, Guido Grossi, Kurt V. Gothelf, Ebbe S. Andersen
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be
Externí odkaz:
https://doaj.org/article/616f157ee0b5469fa10d778ba09eeffb
Autor:
Mette D. E. Jepsen, Steffen M. Sparvath, Thorbjørn B. Nielsen, Ane H. Langvad, Guido Grossi, Kurt V. Gothelf, Ebbe S. Andersen
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018)
In the original version of this Article the last section of the Methods describing Fluorescence microscopy was inadvertently omitted during the production process. This has now been corrected in the PDF and HTML versions of the Article.
Externí odkaz:
https://doaj.org/article/522f639d89774761ae18aa60df82e6cb
Autor:
Guido Grossi, Thorbjørn B. Nielsen, Ebbe Sloth Andersen, Steffen M. Sparvath, Mette D. E. Jepsen, Kurt V. Gothelf, Ane H. Langvad
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Jepsen, M D E, Sparvath, S M, Nielsen, T B, Langvad, A H, Grossi, G, Gothelf, K V & Andersen, E S 2018, ' Development of a genetically encodable FRET system using fluorescent RNA aptamers ', Nature Communications, vol. 9, no. 1, 18 . https://doi.org/10.1038/s41467-017-02435-x
Nature Communications
Jepsen, M D E, Sparvath, S M, Nielsen, T B, Langvad, A H, Grossi, G, Gothelf, K V & Andersen, E S 2018, ' Development of a genetically encodable FRET system using fluorescent RNA aptamers ', Nature Communications, vol. 9, no. 1, 18 . https://doi.org/10.1038/s41467-017-02435-x
Nature Communications
Fluorescent RNA aptamers are useful as markers for tracking RNA molecules inside cells and for creating biosensor devices. Forster resonance energy transfer (FRET) based on fluorescent proteins has been used to detect conformational changes, however,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40601b89ba4af6ad3662ca8f0efda165
http://link.springer.com/article/10.1038/s41467-017-02435-x
http://link.springer.com/article/10.1038/s41467-017-02435-x
Publikováno v:
Selnihhin, D, Sparvath, S M, Preus, S, Birkedal, V & Andersen, E S 2018, ' Multifluorophore DNA Origami Beacon as a Biosensing Platform ', ACS Nano, vol. 12, no. 6, pp. 5699–5708 . https://doi.org/10.1021/acsnano.8b01510
Biosensors play increasingly important roles in many fields, from clinical diagnosis to environmental monitoring, and there is a growing need for cheap and simple analytical devices. DNA nanotechnology provides methods for the creation of sophisticat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e593cde97680c8942156cc65c75c70e
https://pure.au.dk/portal/da/publications/multifluorophore-dna-origami-beacon-as-a-biosensing-platform(193f5400-a55d-4e34-9999-19d8e8cb4797).html
https://pure.au.dk/portal/da/publications/multifluorophore-dna-origami-beacon-as-a-biosensing-platform(193f5400-a55d-4e34-9999-19d8e8cb4797).html
Autor:
Veronica Liv Andersen, Hans Christian Høiberg, Ebbe Sloth Andersen, Steffen M. Sparvath, Jørgen Kjems
Publikováno v:
Høiberg, H C, Sparvath, S M, Andersen, V L, Kjems, J & Andersen, E S 2019, ' An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown ', Biotechnology Journal, vol. 14, no. 1, 1700634 . https://doi.org/10.1002/biot.201700634
The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a three‐dimensional (3D) scaffolded
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba4e1b09c52d78a0e42f339b677b911f
https://pubmed.ncbi.nlm.nih.gov/29802763
https://pubmed.ncbi.nlm.nih.gov/29802763
Publikováno v:
Methods in Molecular Biology ISBN: 9781493964529
RNA nanostructures can be used as scaffolds to organize, combine, and control molecular functionalities, with great potential for applications in nanomedicine and synthetic biology. The single-stranded RNA origami method allows RNA nanostructures to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::239e1307ace361a1a4c52924220c5af6
https://doi.org/10.1007/978-1-4939-6454-3_5
https://doi.org/10.1007/978-1-4939-6454-3_5
Autor:
Thorbjørn B. Nielsen, Mette D. E. Jepsen, Kurt V. Gothelf, Guido Grossi, Ane H. Langvad, Steffen M. Sparvath, Ebbe Sloth Andersen
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018)
Nature Communications
Nature Communications
Fluorescent RNA aptamers are useful as markers for tracking RNA molecules inside cells and for creating biosensor devices. Förster resonance energy transfer (FRET) based on fluorescent proteins has been used to detect conformational changes, however
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b10d2ab0399ec3037a08e67898115509
http://link.springer.com/article/10.1038/s41467-018-03075-5
http://link.springer.com/article/10.1038/s41467-018-03075-5