Zobrazeno 1 - 10
of 64
pro vyhledávání: '"Steffen J, Sahl"'
Autor:
Jan-Erik Bredfeldt, Joanna Oracz, Kamila A. Kiszka, Thea Moosmayer, Michael Weber, Steffen J. Sahl, Stefan W. Hell
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)
Abstract Activation of caged fluorophores in microscopy has mostly relied on the absorption of a single ultraviolet (UV) photon of ≲400 nm wavelength or on the simultaneous absorption of two near-infrared (NIR) photons >700 nm. Here, we show that t
Externí odkaz:
https://doaj.org/article/ba944170f8564bd592f12db06ebc7caa
Near index matching enables solid diffractive optical element fabrication via additive manufacturing
Autor:
Reut Orange kedem, Nadav Opatovski, Dafei Xiao, Boris Ferdman, Onit Alalouf, Sushanta Kumar Pal, Ziyun Wang, Henrik von der Emde, Michael Weber, Steffen J. Sahl, Aleks Ponjavic, Ady Arie, Stefan W. Hell, Yoav Shechtman
Publikováno v:
Light: Science & Applications, Vol 12, Iss 1, Pp 1-12 (2023)
Abstract Diffractive optical elements (DOEs) have a wide range of applications in optics and photonics, thanks to their capability to perform complex wavefront shaping in a compact form. However, widespread applicability of DOEs is still limited, bec
Externí odkaz:
https://doaj.org/article/04f25bfd74024bdeb488c0a3b0795b2b
Autor:
Roman Schmidt, Tobias Weihs, Christian A. Wurm, Isabelle Jansen, Jasmin Rehman, Steffen J. Sahl, Stefan W. Hell
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Minimal photon fluxes (MINFLUX) has enabled molecule-scale resolution in fluorescence microscopy but this had not been shown in standard, broadly applicable microscopy platforms. Here the authors report a solution to allow normal fluorescence microsc
Externí odkaz:
https://doaj.org/article/dc887fa2587b4746875369d1a3177bc1
Autor:
Douglas S. Richardson, Carola Gregor, Franziska R. Winter, Nicolai T. Urban, Steffen J. Sahl, Katrin I. Willig, Stefan W. Hell
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-res
Externí odkaz:
https://doaj.org/article/67dd8700237c48b588b132e8702e7c81
Autor:
Lynn M. Ostersehlt, Daniel C. Jans, Anna Wittek, Jan Keller-Findeisen, Kaushik Inamdar, Steffen J. Sahl, Stefan W. Hell, Stefan Jakobs
Publikováno v:
Nature Methods
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single flu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::936733b06de48b096f838bc0623d2f3c
https://doi.org/10.1038/s41592-022-01577-1
https://doi.org/10.1038/s41592-022-01577-1
Autor:
Mark Bates, Jan Keller-Findeisen, Adrian Przybylski, Andreas Hüper, Till Stephan, Peter Ilgen, Angel R. Cereceda Delgado, Elisa D’Este, Alexander Egner, Stefan Jakobs, Steffen J. Sahl, Stefan W. Hell
Publikováno v:
Nature Methods
Coherent fluorescence imaging with two objective lenses (4Pi detection) enables single-molecule localization microscopy with sub-10 nm spatial resolution in three dimensions. Despite its outstanding sensitivity, wider application of this technique ha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6944854b61b20651295387f5a3d19ad
https://doi.org/10.1038/s41592-022-01465-8
https://doi.org/10.1038/s41592-022-01465-8
Publikováno v:
Journal of Microscopy
Fluorescence nanoscopy methods based on the RESOLFT principle, such as beam-scanning STED nanoscopy, require the co-alignment of optical beams for molecular state (on/off) switching and fluorescence excitation. The complexity and stability of the bea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2c182095da004a48c79dd41747d36f4
https://hdl.handle.net/21.11116/0000-000B-7611-D21.11116/0000-000B-7613-B
https://hdl.handle.net/21.11116/0000-000B-7611-D21.11116/0000-000B-7613-B
Autor:
Tiberiu S. Mihaila, Carina Bäte, Lynn M. Ostersehlt, Jasmin K. Pape, Jan Keller-Findeisen, Steffen J. Sahl, Stefan W. Hell
Publikováno v:
Proceedings of the National Academy of Sciences of the USA
With few-nanometer resolution recently achieved by a new generation of fluorescence nanoscopes (MINFLUX and MINSTED), the size of the tags used to label proteins will increasingly limit the ability to dissect nanoscopic biological structures. Bioorth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55a240e093a7163a87877eb323892bbf
https://pubmed.ncbi.nlm.nih.gov/35858298
https://pubmed.ncbi.nlm.nih.gov/35858298
Autor:
Klaus C. Gwosch, Francisco Balzarotti, Jasmin K. Pape, Philipp Hoess, Jan Ellenberg, Jonas Ries, Ulf Matti, Roman Schmidt, Steffen J. Sahl, Stefan W. Hell
Prakash and Curd provide a re-analysis1 of individual datasets taken from our report2 demonstrating MINFLUX 3D imaging in cells. Their evaluation confirms the unique localization precision provided by MINFLUX2,3 featuring a standard deviation of σ =
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45ec95212b9ae8c0556e9b2fc2eb8765
https://doi.org/10.1101/2022.05.13.491065
https://doi.org/10.1101/2022.05.13.491065
Autor:
Steffen J. Sahl, Jessica Matthias, Kaushik Inamdar, Taukeer A. Khan, Michael Weber, Johannes Broichhagen, Stefan W. Hell
Publikováno v:
Biophysical Journal. 122:451a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d5e3fa1cd390bbf909e0e31126b08e53
https://doi.org/10.1016/j.bpj.2022.11.2428
https://doi.org/10.1016/j.bpj.2022.11.2428