An automated workflow for multi-omics screening of microbial model organisms

Autor: Stefano Donati, Matthias Mattanovich, Pernille Hjort, Simo Abdessamad Baallal Jacobsen, Sarah Dina Blomquist, Drude Mangaard, Nicolas Gurdo, Felix Pacheco Pastor, Jérôme Maury, Rene Hanke, Markus J. Herrgård, Tune Wulff, Tadas Jakočiūnas, Lars Keld Nielsen, Douglas McCloskey

Publikováno v: npj Systems Biology and Applications, Vol 9, Iss 1, Pp 1-12 (2023)

Abstract Multi-omics datasets are becoming of key importance to drive discovery in fundamental research as much as generating knowledge for applied biotechnology. However, the construction of such large datasets is usually time-consuming and expensiv

Externí odkaz: https://doaj.org/article/c89e1030bd6e4de38097125bb0d955c5

Metabolome and proteome analyses reveal transcriptional misregulation in glycolysis of engineered E. coli

Autor: Chun-Ying Wang, Martin Lempp, Niklas Farke, Stefano Donati, Timo Glatter, Hannes Link

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)

Synthetic pathways represent a metabolic burden on host cells. Here the authors engineer Cra-binding sites to prevent misregulation in glycerol and carotenoid overproducing E. coli strains.

Externí odkaz: https://doaj.org/article/e191ae102d374078b676c6a807f7a206

Synthetic biology in Europe: current community landscape and future perspectives

Autor: Stefano Donati, Içvara Barbier, Daniela A. García-Soriano, Stefano Grasso, Paola Handal-Marquez, Koray Malcı, Louis Marlow, Cauã Westmann, Adam Amara

Publikováno v: Donati, S, Barbier, I, García-Soriano, D A, Grasso, S, Handal-Marquez, P, Malcı, K, Marlow, L, Westmann, C & Amara, A 2022, ' Synthetic biology in Europe: current community landscape and future perspectives ', Biotechnology Notes, vol. 3, pp. 54-61 . https://doi.org/10.1016/j.biotno.2022.07.003

Synthetic biology has captivated scientists' imagination. It promises answers to some of the grand challenges society is facing: worsening climate crisis, insufficient food supplies for ever growing populations, and many persisting infectious and gen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29084b00dead5a5659baeddca1d81354
https://doi.org/10.1016/j.biotno.2022.07.003

A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes

Autor: Nicolas T. Wirth, Nicolás Gurdo, Nicolas Krink, Àngela Vidal-Verdú, Stefano Donati, Lorena Férnandez-Cabezón, Tune Wulff, Pablo I. Nikel

Publikováno v: Wirth, N T, Gurdo, N, Krink, N, Vidal-Verdú, À, Donati, S, Férnandez-Cabezón, L, Wulff, T & Nikel, P I 2022, ' A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes ', Metabolic Engineering, vol. 74, pp. 83-97 . https://doi.org/10.1016/j.ymben.2022.09.004

Acetyl-coenzyme A (AcCoA) is a metabolic hub in virtually all living cells, serving as both a key precursor of essential biomass components and a metabolic sink for catabolic pathways for a large variety of substrates. Owing to this dual role, tight

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b04cb828f9bc5f92c84db34a1957163
https://orbit.dtu.dk/en/publications/6072aec0-8aad-441f-8807-98e017a2edd5

Evolution of p53 transactivation specificity through the lens of a yeast-based functional assay.

Autor: Mattia Lion, Ivan Raimondi, Stefano Donati, Olivier Jousson, Yari Ciribilli, Alberto Inga

Publikováno v: PLoS ONE, Vol 10, Iss 2, p e0116177 (2015)

Co-evolution of transcription factors (TFs) with their respective cis-regulatory network enhances functional diversity in the course of evolution. We present a new approach to investigate transactivation capacity of sequence-specific TFs in evolution

Externí odkaz: https://doaj.org/article/f7b4ff61d8ce4a9f927464f7fdcd1c5c