Improved Identification of Protease Cleavage Sites by In-gel Reductive Dimethylation

Autor: Stefanie, Royek, Stefanie, Brück, Jens, Pfannstiel, Annick, Stintzi, Andreas, Schaller

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2581

A critical step in the functional characterization of proteases is the identification of physiologically relevant substrates, which often starts with a collection of candidate proteins. To test these candidates and identify specific processing sites,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4136a71154e27b72a9bfa94d7561a8f4
https://pubmed.ncbi.nlm.nih.gov/36413329

Processing of a plant peptide hormone precursor facilitated by posttranslational tyrosine sulfation

Autor: Stefanie Royek, Martin Bayer, Jens Pfannstiel, Jürgen Pleiss, Gwyneth Ingram, Annick Stintzi, Andreas Schaller

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (16), pp.e2201195119. ⟨10.1073/pnas.2201195119⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2022, 119 (16), ⟨10.1073/pnas.2201195119⟩

Most peptide hormones and growth factors are matured from larger inactive precursor proteins by proteolytic processing and further posttranslational modification. Whether or how posttranslational modifications contribute to peptide bioactivity is sti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fc856f0fcbeb55bcca30d92c7cc7632
https://cnrs.hal.science/hal-03841269/document