A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly

Autor: Sabina Schütz, Ute Fischer, Martin Altvater, Purnima Nerurkar, Cohue Peña, Michaela Gerber, Yiming Chang, Stefanie Caesar, Olga T Schubert, Gabriel Schlenstedt, Vikram G Panse

Publikováno v: eLife, Vol 3 (2014)

Within a single generation time a growing yeast cell imports ∼14 million ribosomal proteins (r-proteins) into the nucleus for ribosome production. After import, it is unclear how these intrinsically unstable and aggregation-prone proteins are targe

Externí odkaz: https://doaj.org/article/3fc393b43bdf4756972fac031e350d97

SUMOylation of the nuclear pore complex basket is involved in sensing cellular stresses

Autor: Lorenz Latta, François Waharte, Surayya Taranum, Stefanie Caesar, Gabriel Schlenstedt, Hanne Folz, Carlos A. Niño, Jean Salamero, Catherine Dargemont

Publikováno v: Journal of Cell Science
Journal of Cell Science, Company of Biologists, 2019, 132 (7), pp.jcs224279. ⟨10.1242/jcs.224279⟩
Journal of Cell Science, 2019, 132 (7), pp.jcs224279. ⟨10.1242/jcs.224279⟩

The nuclear pore complex (NPC) is the major conduit for nucleocytoplasmic transport and serves as a platform for gene regulation and DNA repair. Several nucleoporins undergo ubiquitylation and SUMOylation, and these modifications play an important ro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f91fe07c55d76edbaa30e5dbf41bc14
https://pubmed.ncbi.nlm.nih.gov/30837289

Yeast Mpk1 Cell Wall Integrity Mitogen-activated Protein Kinase Regulates Nucleocytoplasmic Shuttling of the Swi6 Transcriptional Regulator

Autor: Gabriel Schlenstedt, Ki Young Kim, Stefanie Caesar, Andrew W. Truman, David E. Levin

Publikováno v: Molecular Biology of the Cell

Activated Mpk1 MAPK regulates Swi6 nucleocytoplasmic shuttling in a bi-phasic manner. First, it recruits Swi6 to the nucleus non-catalytically by forming a nuclear Mpk1/Swi4 complex to initiate cell wall stress transcription. Then, it phosphorylates

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5eebeac94ee7a2eba022d25fa015f6c7
http://europepmc.org/articles/PMC2861618

A Novel Conserved Nuclear Localization Signal Is Recognized by a Group of Yeast Importins

Autor: Thomas Fries, Gabriel Schlenstedt, Kai Sohn, Christian Betz, Susanne M. Bailer, Stefanie Caesar

Publikováno v: Journal of Biological Chemistry. 282:19292-19301

Nucleo-cytoplasmic transport of proteins is mostly mediated by specific interaction between transport receptors of the importin beta family and signal sequences present in their cargo. While several signal sequences, in particular the classical nucle

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a6479521f09d0a3ac3224748c4e9516
https://doi.org/10.1074/jbc.m700217200

The histones H2A/H2B and H3/H4 are imported into the yeast nucleus by different mechanisms

Autor: Gabriel Schlenstedt, Stefanie Caesar, Markus Greiner

Publikováno v: European Journal of Cell Biology. 83:511-520

Proteins are imported from the cytoplasm into the nucleus by importin beta-related transport receptors. The yeast Saccharomyces cerevisiae contains ten of these importins, but only two of them are essential. After transfer through the nuclear pore, i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f8681e44926d799d227ef90eea506fe
https://doi.org/10.1078/0171-9335-00418