Effective approaches to discover new microbial metabolites in a large strain library

Autor: Guadalupe S. Zarazúa, Marianna Iorio, Arianna Tocchetti, Mitja M Zdouc, Margherita Sosio, Cristina Brunati, Matteo Simone, Stefania Serina, Kristiina Vind, Max Crüsemann, Paolo Monciardini, Stefano Donadio, Sonia I. Maffioli

Publikováno v: Journal of Industrial Microbiology and Biotechnology
Journal of Industrial Microbiology and Biotechnology, 48(3-4)
Journal of Industrial Microbiology and Biotechnology 48 (2021) 3-4

Natural products have provided many molecules to treat and prevent illnesses in humans, animals and plants. While only a small fraction of the existing microbial diversity has been explored for bioactive metabolites, tens of thousands of molecules ha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e41e7f3c4b8d748c36a078b7fa82197
https://doi.org/10.1093/jimb/kuab017

Metabolomic investigation of the pseudouridimycin producer, a prolific streptomycete

Autor: Stefania Serina, Sonia I. Maffioli, Davatgarbenam S, Matteo Simone, Marianna Iorio, Mitja M Zdouc, Richard H. Ebright, Margherita Sosio, Stefano Donadio, Criscenzo P

Publikováno v: BioRxiv
bioRxiv

We report a metabolomic analysis of Streptomyces sp. ID38640, a soil isolate that produces the bacterial RNA polymerase inhibitor pseudouridimycin. The analysis was performed on the wild type and on ten different pum mutants blocked at different step

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22741053bec7faf9864d6e5c74c82865
https://doi.org/10.1101/2020.11.05.369249

Antibacterial nucleoside-analog inhibitor of bacterial RNA polymerase: pseudouridimycin

Autor: Giuseppe Facchetti, Hans-Georg Sahl, David Degen, Paola Guglierame, Carlo Mazzetti, Sonia I. Maffioli, Stefania Serina, Petra Kaltofen, Giancarlo Del Gatto, Thomas Carzaniga, Alina Iulia Chiriac, Paolo Monciardini, Stefano Donadio, Yu Zhang, Gianni Dehò, Richard H. Ebright, Gianpaolo Candiani

There is an urgent need for new antibacterial drugs effective against bacterial pathogens resistant to current drugs1–2. Nucleoside-analog inhibitors (NAIs) of viral nucleotide polymerases have had transformative impact in treatment of HIV3and HCV4

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f553c70921bb0c5724e6bb6983974d6c

Targets and assays for discovering novel antibacterial agents

Autor: Lucia Carrano, Adolfo Soffientini, Margherita Sosio, Stefano Donadio, Letizia Brandi, Nicoletta Montanini, Stefania Serina, Elena Raimondi, Claudio O. Gualerzi

Publikováno v: Journal of Biotechnology. 99:175-185

The increasing frequency of nosocomial infections due to multi-resistant pathogens exerts a significant toll and calls for novel and better antibiotics. Different approaches can be used in the search for novel antibiotics acting on drug-resistant bac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51259b434a7e1c61deb50d269b054a95
https://doi.org/10.1016/s0168-1656(02)00208-0

Identification of Two Replicons in Phage-Plasmid P4

Autor: Stefania Serina, Daniela Ghisotti, Arianna Tocchetti, Gianni Dehò, Susanna Terzano

Publikováno v: Virology. 245:344-352

DNA replication of phage-plasmid P4 proceeds bidirectionally from the ori1 site (previously named ori), but requires a second cis-acting region, crr. Replication depends on the product of the P4 alpha gene, a protein with primase and helicase activit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4cb9567b21d577dfbd2533e347a607b
https://doi.org/10.1006/viro.1998.9167