Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Stefan Tippmann"'
Autor:
Josefine Anfelt, Jens Nielsen, Stefan Tippmann, Mathias Uhlén, Verena Siewers, Jacqueline M. Rand, Florian David, Elton P. Hudson
Publikováno v:
ACS Synthetic Biology. 6:19-28
Enzyme fusions have been widely used as a tool in metabolic engineering to increase pathway efficiency by reducing substrate loss and accumulation of toxic intermediates. Alternatively, enzymes can be colocalized through attachment to a synthetic sca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06157834575affbef615ef063a61b31a
https://doi.org/10.1021/acssynbio.6b00109
https://doi.org/10.1021/acssynbio.6b00109
Autor:
Suwanee Jansa-Ard, Raffaele Nicastro, Marieke F. Buffing, Jens Nielsen, Stefan Tippmann, José L. Martínez, Sakda Khoomrung
Publikováno v:
Analytical Chemistry Research, Vol 6, Iss C, Pp 9-16 (2015)
Khoomrung, S, Martinez Ruiz, J L, Tippmann, S, Jansa-Ard, S, Buffing, M F, Nicastro, R & Nielsen, J 2015, ' Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization ', Analytical Chemistry Research, vol. 6, pp. 9-16 . https://doi.org/10.1016/j.ancr.2015.10.001
Khoomrung, S, Martinez Ruiz, J L, Tippmann, S, Jansa-Ard, S, Buffing, M F, Nicastro, R & Nielsen, J 2015, ' Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization ', Analytical Chemistry Research, vol. 6, pp. 9-16 . https://doi.org/10.1016/j.ancr.2015.10.001
We present an improved extraction and derivatization protocol for GC-MS analysis of amino/non-amino acids in Saccharomyces cerevisiae. Yeast cells were extracted with chloroform: aqueous-methanol (1:1, v/v) and the resulting non-polar and polar extra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d3cef950a4b8984edc71eadf511dbcc
https://doi.org/10.1016/j.ancr.2015.10.001
https://doi.org/10.1016/j.ancr.2015.10.001
Publikováno v:
Biotechnology and Bioengineering. 113:72-81
Terpenes have various applications as fragrances, cosmetics and fuels. One of the most prominent examples is the sesquiterpene farnesene, which can be used as diesel substitute in its hydrogenated form farnesane. Recent metabolic engineering efforts
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cd503248141d87a30c93aa20b7bd3889
https://doi.org/10.1002/bit.25683
https://doi.org/10.1002/bit.25683
Publikováno v:
Talanta. 146
Organic solvents are widely used in microbial fermentations to reduce gas stripping effects and capture hydrophobic or toxic compounds. Reliable quantification of biochemical products in these overlays is highly challenging and practically difficult.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0565b98537927649bf36c47db687cb43
https://pubmed.ncbi.nlm.nih.gov/26695240
https://pubmed.ncbi.nlm.nih.gov/26695240
Publikováno v:
Biotechnology and bioengineering. 113(1)
Terpenes have various applications as fragrances, cosmetics and fuels. One of the most prominent examples is the sesquiterpene farnesene, which can be used as diesel substitute in its hydrogenated form farnesane. Recent metabolic engineering efforts
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9ef897ba36c5bb02c49ad34d2a834a6d
https://pubmed.ncbi.nlm.nih.gov/26108688
https://pubmed.ncbi.nlm.nih.gov/26108688
Publikováno v:
Tippmann, S, Chen, Y, Siewers, V & Nielsen, J 2013, ' From flavors and pharmaceuticals to advanced biofuels: Production of isoprenoids in Saccharomyces cerevisiae ', Biotechnology Journal, vol. 8, no. 12, pp. 1435-1444 . https://doi.org/10.1002/biot.201300028
Isoprenoids denote the largest group of chemicals in the plant kingdom and are employed for a wide range of applications in the food and pharmaceutical industry. In recent years, isoprenoids have additionally been recognized as suitable replacements
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8e1cd4767999169424262f5f2d51f73
https://orbit.dtu.dk/en/publications/62f1acef-9930-41f3-8bf1-7dd9dd212ce6
https://orbit.dtu.dk/en/publications/62f1acef-9930-41f3-8bf1-7dd9dd212ce6
Publikováno v:
New Biotechnology. 31:S165
The yeast Saccharomyces cerevisiae is a widely used platform for the production of heterologous proteins of either therapeutic or industrial interest. However, heterologous protein productivity is often low due to limitations of the selected host str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::729ec29c89c93286c5d14c51cf7c5108
https://doi.org/10.1016/j.nbt.2014.05.2032
https://doi.org/10.1016/j.nbt.2014.05.2032