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pro vyhledávání: '"Stefan Schleper"'
Autor:
Jürgen Hubbuch, Juliane Diehm, Laura Rolinger, Jessica Chow-Hubbertz, Martin Heitmann, Matthias Rüdt, Stefan Schleper
Publikováno v:
Analytical and Bioanalytical Chemistry. 412:2123-2136
Ultrafiltration/diafiltration (UF/DF) plays an important role in the manufacturing of biopharmaceuticals. Monitoring critical process parameters and quality attributes by process analytical technology (PAT) during those steps can facilitate process d
Autor:
Gottfried J. Palm, Jacek Lubkowski, Christian Derst, Alexander Wlodawer, Stefan Schleper, Klaus-Heinrich Röhm
Publikováno v:
FEBS Letters. 390:211-216
Escherichia coli asparaginase II catalyzes the hydrolysis of l-asparagine to l-aspartate via a threonine-bound acylenzyme intermediate. A nearly inactive mutant in which one of the active site threomines, Thr-89, was replaced by valine was constructe
Publikováno v:
Biochimica et biophysica acta. 1481(2)
Asparaginase II from Escherichia coli is well-known member of the bacterial class II amidohydrolases. Enzymes of this family utilize a peculiar catalytic mechanism in which a pair of threonine residues play pivotal roles. Another common feature is a