Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Stefan Helm"'
Autor:
Julia Grimmer, Stefan Helm, Dirk Dobritzsch, Gerd Hause, Gerta Shema, René P. Zahedi, Sacha Baginsky
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Most chloroplast proteins are imported from the cytosol and thus transiently exposed to the cytosolic proteasome. Here the authors show that impairment of the cytosolic proteasome can elevate precursor protein abundance and photosynthetic activity su
Externí odkaz:
https://doaj.org/article/69490422533c4a51abd07417825ad799
Publikováno v:
PLoS ONE, Vol 14, Iss 3, p e0213364 (2019)
We report a systematic analysis of chloroplast high-molecular mass protein complexes using a combination of native gel electrophoresis and absolute protein quantification by MSE. With this experimental setup, we characterized the effect of the tic56-
Externí odkaz:
https://doaj.org/article/6012c54e44b240be94dad4120a76dff4
Autor:
Anna Schönberg, Elena Bergner, Stefan Helm, Birgit Agne, Beatrix Dünschede, Danja Schünemann, Mike Schutkowski, Sacha Baginsky
Publikováno v:
PLoS ONE, Vol 9, Iss 10, p e108344 (2014)
We report the development of a peptide microarray based on previously determined phosphorylation sites in chloroplast proteins. Altogether, 905 peptides were spotted as 15mers in nine replicates onto glass slides. We used the microarray for in vitro
Externí odkaz:
https://doaj.org/article/7393be6b1f054a9fbc5a435987b1de3a
Autor:
Fränze Müller, Stefan Helm, Birgit Agne, Sacha Baginsky, Nina Pötzsch, Anja Rödiger, Dirk Dobritzsch
Publikováno v:
The Plant Journal. 105:1431-1442
We report here a detailed analysis of the proteome adjustments that accompany chromoplast differentiation from chloroplasts during bell-pepper fruit ripening. While the two photosystems are disassembled and their constituents degraded, the cytochrome
Autor:
Thomas Pfannschmidt, Stefan Helm, Birgit Agne, Elena Bergner, Wolfgang Hoehenwarter, Gerd Hause, Domenika Thieme, Alisdair R. Fernie, Sacha Baginsky, Anja Rödiger, Saleh Alseekh, Johann Galonska
Publikováno v:
Plant Journal
Plant Journal, 2020, 104 (2), pp.546-558. ⟨10.1111/tpj.14944⟩
Plant Journal, Wiley, 2020, 104 (2), pp.546-558. ⟨10.1111/tpj.14944⟩
The Plant Journal
Plant Journal, 2020, 104 (2), pp.546-558. ⟨10.1111/tpj.14944⟩
Plant Journal, Wiley, 2020, 104 (2), pp.546-558. ⟨10.1111/tpj.14944⟩
The Plant Journal
International audience; Casein kinase 2 is a ubiquitous protein kinase that has puzzled researchers for several decades because of its pleiotropic activity. Here, we set out to identify the in vivo targets of plastid casein kinase 2 (pCK2) in Arabido
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30357b2438ae85e51770cd39d9707dc8
https://hal.science/hal-02974315
https://hal.science/hal-02974315
Autor:
Stefan Helm, Praveen Krishnamoorthy, Mareike Heilmann, Ralph Golbik, Sacha Baginsky, Dirk Dobritzsch, Wilhelm Menzel, Ingo Heilmann, Irene Stenzel, Justin Lee, Franziska Hempel, Wolfgang Hoehenwarter
Publikováno v:
The Plant Cell. 29:3030-3050
An apical plasma membrane domain enriched in the regulatory phospholipid phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] is critical for polar tip growth of pollen tubes. How the biosynthesis of PtdIns(4,5)P2 by phosphatidylinositol 4-phosphate
Autor:
Wolfgang Hoehenwarter, Stefan Helm, Anja Rödiger, Sacha Baginsky, Johann Galonska, Petra Majovsky, Gideon Christ, Anna Schönberg, Domenika Thieme, Wiebke Mehwald
Publikováno v:
The Plant Journal. 90:1176-1186
Summary The thylakoid-associated kinases STN7 and STN8 are involved in short- and long-term acclimation of photosynthetic electron transport to changing light conditions. Here we report the identification of STN7/STN8 in vivo targets that connect pho
Autor:
Stefan, Helm, Sacha, Baginsky
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1696
Label-free peptide quantification is a promising approach for the large-scale characterization of proteome dynamics at low cost. Here, we describe a method for absolute label-free quantification using an untargeted approach for peptide fragmentation
Publikováno v:
Plant Physiology. 169:996-1000
Tyr phosphorylation is a controversial issue in plant phosphoproteomics, ever since early analyses reported up to 5% Tyr phosphorylation in Arabidopsis ( Arabidopsis thaliana ), despite the lack of a classical Tyr kinase in the Arabidopsis genome ([S
Autor:
Cláudia Pascoal, Fernanda Cássio, Gerd-Joachim Krauss, Dirk Dobritzsch, Dietmar Schlosser, Melanie Dobritzsch, Arunava Pradhan, Sahadevan Seena, Stefan Helm, Katharina Gerth
Publikováno v:
Environmental Toxicology and Chemistry. 34:923-930
FEDER-POFC-COMPETE and the Portuguese Foundation for Science and Technology (PEst-OE/BIA/UI4050/2014, PTDC/AAC-AMB/121650/2010, FCT-DAAD-2010-2011) supported this work and A. Pradhan (SFRH/BD/45614/2008).