Výsledky vyhledávání - "Stefan Graupner"

Requirement of Novel Competence Genes pilT and pilU of Pseudomonas stutzeri for Natural Transformation and Suppression of pilT Deficiency by a Hexahistidine Tag on the Type IV Pilus Protein PilAI

Autor: Monika Sohni, Nicole Weger, Stefan Graupner, Wilfried Wackernagel

Publikováno v: Journal of Bacteriology. 183:4694-4701

The ubiquitous species Pseudomonas stutzeri has type IV pili, and these are essential for the natural transformation of the cells. An absolute transformation-deficient mutant obtained after transposon mutagenesis had an insertion in a gene which was

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6ec916bcac41148195c94d9bf49f56c8
https://doi.org/10.1128/jb.183.16.4694-4701.2001

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Identification and characterization of novel competence genes comA and exbB involved in natural genetic transformation of Pseudomonas stutzeri

Autor: Stefan Graupner, Wilfried Wackernagel

Publikováno v: Research in Microbiology. 152:451-460

After transposon mutagenesis we identified a novel gene (comA) of Pseudomonas stutzeri which is essential for natural genetic transformation. The putative amino acid sequence is similar to ComA orthologs of other transformable bacteria including Neis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b48d56f53a23b3d946357f037b4fd2a
https://doi.org/10.1016/s0923-2508(01)01218-9

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Pseudomonas stutzeri Has Two Closely Related pilA Genes (Type IV Pilus Structural Protein) with Opposite Influences on Natural Genetic Transformation

Autor: Wilfried Wackernagel, Stefan Graupner

Publikováno v: Journal of Bacteriology. 183:2359-2366

Pseudomonas stutzeri has type IV pili for which the pilA gene (here termed pilAI ) provides the structural protein and which are required for DNA uptake and natural genetic transformation. Downstream of pilAI we identified a gene, termed pilAII , cod

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1072af962071ade3c6d160d199dbdeae
https://doi.org/10.1128/jb.183.7.2359-2366.2001

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Type IV Pilus Genes pilA and pilC of Pseudomonas stutzeri Are Required for Natural Genetic Transformation, and pilA Can Be Replaced by Corresponding Genes from Nontransformable Species

Autor: Verena Frey, Wilfried Wackernagel, Rozita Hashemi, Michael G. Lorenz, Stefan Graupner, Gudrun Brandes

Publikováno v: Journal of Bacteriology. 182:2184-2190

Pseudomonas stutzeri lives in terrestrial and aquatic habitats and is capable of natural genetic transformation. After transposon mutagenesis, transformation-deficient mutants were isolated from a P. stutzeri JM300 strain. In one of them a gene which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::24ed3d813baefd1f4a11387cba1d893d
https://doi.org/10.1128/jb.182.8.2184-2190.2000

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Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli

Autor: Wilfried Wackernagel, Stefan Graupner

Publikováno v: Applied and Environmental Microbiology. 62:1839-1841

Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::319ee346aa5e984f289bea803484f852
https://doi.org/10.1128/aem.62.5.1839-1841.1996

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A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri

Autor: Stefan Graupner, Wilfried Wackernagel

Publikováno v: Biomolecular engineering. 17(1)

A series of expression vectors for gram-negative bacteria was constructed which combine broad-host-range, inducible expression from the tac promoter and diverse antibiotic resistance determinants. The tac promoter activity and the repression by lacIq

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad091a8140d7b1834995a0e1bc4e96ae
https://pubmed.ncbi.nlm.nih.gov/11042472

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